Optimization of Storage Medium for Hematological Reference Samples in External Quality Assessment

Abstract

Hematological reference samples for external quality assessment (EQA) in hematology are essential for many laboratories in Vietnam, however, the prices are high along with short storing time. This study attempted to establish an optimal formula for the manufacture of hematological reference samples for hematological EQA programs. Human red blood cells (RBCs) were mixed with goose RBCs (pseudo-leucocytes) and goat RBCs (pseudo-platelets) as alternatives for white blood cells (WBCs) and platelets (PLTs), respectively, in different formulas of storage media. The optimal formula was obtained using response surface methodology–central composite design (RSM-CCD). Three main factors affecting the concentrations of RBCs were determined by Plackett–Burmann matrix. The optimal concentrations determined by RSM-CCD were 46% serum, 0.44 g/L neomycin sulfate, and 2.5% glycerol. The model predicted that the maximum number of human RBCs was 4.1 × 1012 cells/L, pseudo-leucocytes was 6.4 × 109 cells/L, and pseudo-platelets was 188 × 109 cells/L. The practical concentrations of RBCs, pseudo-leucocytes, and pseudo-platelets were 4.21 × 1012 cells/L, 6.4 × 109 cells/L, and 187 × 109 cells/L, respectively, which showed 98.93% similarity with theoretical data. This result could be a premise for further study to improve manufacturing of hematological reference samples for hematology EQA programs.

Determination of the Assigned Values of Blood Cells by an Impedance Method for Hematological Reference Samples Used in Hematology External Quality Assessment (EQA) Programs

“…In previous research, we have fixed human RBCs, used goose and goat RBCs to replace human WBCs (pseudo-leucocytes), and used human PLTs (pseudo-platelets). The optimal formula was obtained using response surface methodology–central composite design (RSM-CCD); the results determined the optimal formulas for the blood cell of storage medium [ 8 ]. The sample life after optimization was determined to be 3 months.…”

“…In previous research, we have fixed human RBCs, used goose and goat RBCs to replace human WBCs (pseudo-leucocytes), and used human PLTs (pseudo-platelets). We have determined the optimal storage medium formulas for the three types of red blood cells and the sample shelf-life was determined to be 3 months [ 8 ]. In this research, we performed threshold determination for three concentrations levels (high, normal, and low) in hematology analysis.…”

Sử Dụng Phương Pháp Đo Quang Để Xác Định Giá Trị Ấn Định Của Mẫu Sinh Phẩm Huyết Học Trong Kiểm Tra Chất Lượng Xét Nghiệm Huyết Học

“…Các nghiên cứu trước, chúng tôi đã cố định được hồng cầu người; dùng hồng cầu ngỗng, dê để thay thế bạch cầu, tiểu cầu người. Sử dụng thiết kế Plackett-Burman, phương pháp RSM-CCD để sàng lọc, tối ưu hóa các yếu tố ảnh hưởng, kết quả đã xác định các công thức tối ưu cho môi trường bảo quản tế bào máu [4]. Đồng thời, đánh giá mô hình bằng thực nghiệm đã xác định mẫu ổn định trong thời gian 3 tháng, ở nhiệt độ 2-8 o C ở điều kiện phòng thí nghiệm và điều kiện vận chuyển.…”