Optimization of Submerged Fermentation Medium for Matrine Production by <i>Aspergillus terreus</i>, an Endophytic Fungus Harboring Seeds of <i>Sophora flavescens</i>, Using Response Surface Methodology

Zhang, Qiang; Li, Yujuan; Xu, Fangxue; Zheng, Mengmeng; Xi, Xiaozhi; Zhang, Xuelan; Han, Chunchao

doi:10.5941/myco.2017.45.2.90

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…In this study, for the first time, the plant growth-promoting activities of endophytes isolated from various organs of S. flavescens (shrubby sophora) were analyzed in order to compare and evaluate their effects on root growth, biomass and the photosynthetic pigments (chlorophyll-a, -b and carotenoids) in A. thaliana. Previous studies on fungal endophytes inhabiting the species S. flavescens comprised only two fungal genera, Aspergillus and Penicillium [14][15][16]. Based on our examinations, the fungal isolates represented the genera of Alternaria, Didymella, Fusarium and Xylogone, which have not been previously reported as fungal endophytes of S. flavescens.…”

Section: Discussionmentioning

confidence: 50%

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Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

et al. 2020

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Endophytic fungi in symbiotic association with their host plant are well known to improve plant growth and reduce the adverse effects of both biotic and abiotic stresses. Therefore, fungal endophytes are beginning to receive increased attention in an effort to find growth-promoting strains that could be applied to enhance crop yield and quality. In our study, the plant growth-promoting activities of endophytic fungi isolated from various parts of Sophora flavescens (a medicinally important plant in Mongolia and China) have been revealed and investigated. Fungal isolates were identified using molecular taxonomical methods, while their plant growth-promoting abilities were evaluated in plate assays. Altogether, 15 strains were isolated, representing the genera Alternaria, Didymella, Fusarium and Xylogone. Five of the isolates possessed phosphate solubilization activities and twelve secreted siderophores, while all of them were able to produce indoleacetic acid (IAA) in the presence or absence of tryptophan. The endogenous and exogenous accumulation of IAA were also monitored in liquid cultures using the HPLC-MS/MS technique to refine the plate assay results. Furthermore, for the highest IAA producer fungi, the effects of their extracts were also examined in plant bioassays. In these tests, the primary root lengths of the model Arabidopsis thaliana were increased in several cases, while the biomasses were significantly lower than the control IAA treatment. Significant alterations have also been detected in the photosynthetic pigment (chlorophyll-a, -b and carotenoids) content due to the fungal extract treatments, but these changes did not show any specific trends.

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Section: Discussionmentioning

confidence: 50%

“…were isolated from S. flavescens and their bioactive compounds were purified and identified [14,15]. According to Zhang et al, the A. terreus strain was also isolated from the seeds of this plant and the fungal production of a host metabolite was confirmed and optimized [16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

et al. 2020

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“…The Box–Behnken factorial design is shown in Table 3 . The software Design-Expert 8.0.5.0 trial was utilized in experimental design, data analysis, and quadratic equation construction [ 40 ]. Independent variables were coded according to the following formula.…”

Section: Methodsmentioning

confidence: 99%

“…Independent variables were coded according to the following formula. X i = (X i − X 0 /ΔX i (i = 1, 2, 3) where X i is the coded values of X i , X i is the real value of an independent variable, X 0 is the real value of independent variables on the center point and ΔX i is the step change value [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Optimization of Fermentation Condition for Echinacoside Yield Improvement with Penicillium sp. H1, an Endophytic Fungus Isolated from Ligustrum lucidum Ait Using Response Surface Methodology

Cao

Cui

et al. 2018

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(1) Background: Application of echinacoside has become increasingly important for its significant biological activities. However, there are many disadvantages in existing synthesis methods such as contaminating the environment, harsh reaction conditions and so on. Therefore, it is urgent to invent a novel alternative method that can increase the yield of echinacoside. (2) Methods: In this study, we isolated and purified an endophyte from the leaves of Ligustrum lucidum Ait. Then, we improved the yield of echinacoside by optimizing the fermentation condition with an endophytic fungus. Penicillium sp. H1 was isolated from Ligustrum lucidum Ait. In addition, response surface methodology was used to optimize the fermentation condition. (3) Results: The results indicate that the maximal yield of echinacoside (37.16 mg/L) was obtained when inoculation rate, temperature and days were 13.98%, 27.85 °C and 26.06 days, respectively. The yield of echinacoside was 150.47 times higher under the optimal conditions than under the control conditions. The results indicate that the yield of echinacoside could be improved with endophytic fermentation by optimizing the fermentation condition. We provide an alternative method for echinacoside production by endophytic fermentation in this paper. It may have a profound effect on the application of echinacoside.

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“…Optimization of fermentation medium. The fundamental fermentation parameters were as follows: pH = 7, 32˚C, 300 r/min, and aerobic incubation for 72 h [14]. Each of the fermentation media was used as a single factor to produce MRYP.…”

Section: Animals and Strainsmentioning

confidence: 99%

Production, purification, characterization, and biological properties of Rhodosporidium paludigenum polysaccharide

et al. 2021

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The yield of marine red yeast polysaccharide (MRYP) obtained from Rhodosporidium paludigenum was increased by optimizing fermentation conditions, and the pure polysaccharide was extracted by column chromatography. The molecular weight of pure MRYP and the ratio of mannose to glucose in components of MRYP were determined. Antioxidant and antibacterial abilities of MRYP were investigated in vitro and in vivo. The optimal fermentation parameters were as follows: Medium 4, pH = 6.72, temperature = 30.18°C, blades speed = 461.36 r/min; the optimized yield reached 4323.90 mg/L, which was 1.31 times the original yield. The sequence of factors that affected the MRYP yield was the blades speed>pH>temperature. The main components of MRYP were MYH-1 and MYH-2. The molecular weights of MYH-1 and MYH-2 were 246.92 kDa and 21.88 kDa, respectively; they accounted for 53.60% and 28.75% of total polysaccharide. In MYH-1 and MYH-2, the proportion of glucose and mannose accounted for 46.94%, 38.46%, and 67.10%, 7.17%, respectively. In vitro, the ability of scavenging DPPH•, •OH, and •O2− radical was 32.26%, 24.34%, and 22.09%; the minimum inhibitory concentration (MIC) of MRYP was 480 μg/mg. In vivo, MRYP improved the lambs’ body weight, antioxidant enzyme activity, and the number of probiotics, but it reduced the feed/gain (F/G) ratio and the number of pathogenic bacteria in 60-days-old lambs.

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Optimization of Submerged Fermentation Medium for Matrine Production by Aspergillus terreus, an Endophytic Fungus Harboring Seeds of Sophora flavescens, Using Response Surface Methodology

Cited by 13 publications

References 37 publications

Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

Optimization of Fermentation Condition for Echinacoside Yield Improvement with Penicillium sp. H1, an Endophytic Fungus Isolated from Ligustrum lucidum Ait Using Response Surface Methodology

Production, purification, characterization, and biological properties of Rhodosporidium paludigenum polysaccharide

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