Optimization of sulfated modification conditions of tremella polysaccharide and effects of modifiers on cellular infectivity of NDV

The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity

“…The cell A 570 value is an index to reflect antiviral activity of polysaccharides in this experiment, and positive correlation with the number of living cells [10]. The larger A 570 value, the more living cells and the stronger polysaccharide antiviral activity is.…”

“…The biological activity of polysaccharide mainly depends on its molecular structure, especially when some chemical group are inserted, the flexibility and space structures of polysaccharide chains are changed, which will make polysaccharide activity change or generate new activity. Therefore, the chemical modification becomes a hot spot in polysaccharides research field [7][8][9][10].…”

Sulfated polysaccharides from Cyclocarya paliurus reduce H 2 O 2 -induced oxidative stress in RAW264.7 cells

“…The addition of chlorosulfonic acid and high temperature of esterification could be considered as main factors for this transformation [16]. This phenomenon reflected the importance of appropriate reaction conditions to the sulfated modification for the aim of higher biological activity [32]. Ma et al [33] reported the SEM of the native mushroom Inonotus obliquus polysaccharide and its sulfation derivatives, and found that sulfated polysaccharides showed as irregular particles, whereas the surface of native polysaccharides appeared as fragments.…”

Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide

“…Four polysaccharides were modified by CSA-Pry method [18] respectively under the optimal modification conditions according to our previous researches. The conditions for OPS were the ratio of chlorosulfonic acid to pyridine of 1:4, the reaction temperature of 60 • C and the reaction time of 2 h; for JPS, the ratio of chlorosulfonic acid to pyridine of 1:4, the reaction temperature of 85 • C and the reaction time of 1 h. OPS t , OPS 80 , JPS t and JPS 50 were respectively modified to obtain, sulfated sOPS t , sOPS 80 , sJPS t and sJPS 50 .…”