Optimization of supercritical fluid extraction and HPLC identification of wedelolactone from Wedelia calendulacea by orthogonal array design

Abstract

The purpose of this work is to provide a complete study of the influence of operational parameters of the supercritical carbon dioxide assisted extraction (SC CO2E) on yield of wedelolactone from Wedelia calendulacea Less., and to find an optimal combination of factors that maximize the wedelolactone yield. In order to determine the optimal combination of the four factors viz. operating pressure, temperature, modifier concentration and extraction time, a Taguchi experimental design approach was used: four variables (three levels) in L9 orthogonal array. Wedelolactone content was determined using validated HPLC methodology. Optimum extraction conditions were found to be as follows: extraction pressure, 25 MPa; temperature, 40 °C; modifier concentration, 10% and extraction time, 90 min. Optimum extraction conditions demonstrated wedelolactone yield of 8.01 ± 0.34 mg/100 g W. calendulacea Less. Pressure, temperature and time showed significant (p < 0.05) effect on the wedelolactone yield. The supercritical carbon dioxide extraction showed higher selectivity than the conventional Soxhlet assisted extraction method.

Enhancement of Skin Anti-Inflammatory Activities of Eclipta prostrata L. from the Ultrasonic Extraction Process

“…This amount was two times higher than that in ultrasonic process, as well as that in the other reported results in the range of 100-400 mg/100 g [23,24]. This result indicates that the UE may play an important role in improving the extraction yield of wedelolactone because of the minimal destruction of heat-labile wedelolactone caused by the low temperature extraction process, even at low temperature [25]. Therefore, the extract from the ultrasonic process is expected to have good antioxidant activities because wedelolactone is a family of coumarin known to have high antioxidant activity and anti-inflammatory efficacy [15,26].…”

“…This result could be attributed to the synergistic effects of wedelolactone and other components, which were increased through ultrasonication, and affected the anti-inflammatory activity so that the anti-inflammatory of E. prostrata L. increased. In addition, as previous studies identified that wedelolactone-a component of E. prostrata L.-is rapidly destroyed at temperatures higher than 60 • C, the low-temperature ultrasonic extraction process presented in this study could be an alternative process that minimizes the destruction of wedelolactone and enhances the extraction efficiency [25].…”

Optimization of Process Parameters forε-Polylysine Production by Response Surface Methods

“…Test. Orthogonal array test has been successfully used in the optimization of bioproduct extractions from fermentation broths and other plant materials [36][37][38]. In the present study, based on the single-factor analysis results, a Significant order > > > >…”

Optimaztion of Gamma-Decalactone Production by Yeast Yarrowia Lipolytica Using the Taguchi Method

“…Furthermore, a little additional γ-decalactone was produced in media containing greater than 20 g/L of nitrogen concentrations. The function of these complex protein sources is important to increase cell growth and minimize toxic effect in biotransformation (Maume & Cheetham, 1991;Patil et al, 2014).…”