Optimization of Surfactin Production by Bacillus subtilis Isolate BS5

Abdel-Mawgoud, Ahmad Mohammad; Aboulwafa, Mohammad M.; Hassouna, Nadia A.

doi:10.1007/s12010-008-8155-x

Cited by 135 publications

(78 citation statements)

References 40 publications

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“…Molasses-mineral salts medium (MMSM) is a medium optimized for surfactin production by B. subtilis isolate BS5 that was previously developed by [10]. the composition of MMSM was as follows (per liter): molasses (160 ml); NaNO 3 (5 g), ZnSO 4 (0.16 g), FeCl 3 6H 2 O (0.27 g), MnSO 4 H 2 O (0.017 g), MgSO 4 7H 2 O (0.4 g), NaCl (1 g), KCl (1 g), H 3 PO 4 85% (10 ml), and distilled H 2 O to 1,000 ml, and the pH was adjusted to 7.2 with KOH pellets.…”

Section: Culture Mediamentioning

confidence: 99%

Characterization of Surfactin Produced by Bacillus subtilis Isolate BS5

Abdel-Mawgoud

Aboulwafa

Hassouna

2008

Appl Biochem Biotechnol

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134

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Physical and chromatographic characterization of the surfactin biosurfactant produced by Bacillus subtilis isolate BS5 has been conducted to study its potentiality for industrial application. The crude extract of test surfactin appeared as off-white to buff flake-like amorphous residue with bad odor similar to sour pomegranate. Test surfactin showed solubility in aqueous solution at pH>5 with optimum solubility at pH 8-8.5. It was also soluble in organic solvents like ethanol, acetone, methanol, butanol, chloroform, and dichloromethane. Surfactin crystals appeared rectangular with blunt corners and were arranged perpendicular to each other making a plus sign. Extracted surfactin showed high surface activity, as it could lower the surface tension of water from about 70 to 36 mN/m at approximately 15.6 mg/l. Moreover, test surfactin exhibited excellent stabilities at high temperatures (100 degrees C for up to 1 h at and autoclaving at 121 degrees C for 10 min), salinities (up to 6% NaCl), and over a wide range of pH (5-13). Test surfactin in the cell-free supernatant or crude culture broth forms showed high emulsification indices against kerosene (62.5% and 59%, respectively), diesel (62.5% and 66%, respectively), and motor oil (62% and 66%, respectively). These characters can effectively make test surfactin, in its crude forms, a potential candidate for the use in bioremediation of hydrocarbon-contaminated sites or in the petroleum industry. Chromatographic characterization of test surfactin, using high-performance liquid chromatography technique, revealed that the extracted surfactin contained numerous isoforms, of which six were found in the standard surfactin preparation (Fluka). Additional peaks appeared in the test surfactin and not in the standard one. These peaks may correspond to new surfactin isoforms that may be present in the test surfactin produced by B. subtilis isolate BS5.

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Section: Culture Mediamentioning

confidence: 99%

Characterization of Surfactin Produced by Bacillus subtilis Isolate BS5

Abdel-Mawgoud

Aboulwafa

Hassouna

2008

Appl Biochem Biotechnol

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134

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“…Molasses of different kinds viz., sugarcane molasses, blackstrap, and beet-molasses are widely used in the fermentation industry for the production of pullulan [22], succinic acid [23], and surfactin [24]. However, the production of SL using this raw material, which is considered much cheaper than others, has not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

Production, Characterization, and Properties of Sophorolipids from the Yeast Candida bombicola using a Low-cost Fermentative Medium

Daverey

Pakshirajan

2008

Appl Biochem Biotechnol

145

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The yeast Candida bombicola produces biosurfactant with properties akin to those of sophorolipid (SL) group of compounds. In the present work, the yeast was shown to produce 63.7 g l(-1) SL when grown on a cheap fermentative medium containing sugarcane molasses, yeast extract, urea, and soybean oil. The partially purified SL was characterized and confirmed by Fourier-transform infrared (FT-IR) spectroscopy, 1H and 13C nuclear magnetic resonance (NMR) and liquid chromatography-mass spectroscopy (LC-MS) analysis. The critical micelle concentration (CMC) and minimum surface tension of the produced SL in aqueous solution were found to be 59.43 mg l(-1) and 34.15 m Nm(-1), respectively. The emulsification activity and stability with kerosene oil and organic solvents viz. xylene, benzene, and hexadecane were also tested using the produced SL, which yielded better results compared to those reported in the literature.

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“…1 -Effect of agitation in rhamnolipid production as substrate 19 remained unaffected by aeration rate. On the other hand, Abdel-Mawgoud et al 20 reported that maximum surfactin production by Bacillus subtilis BS5 occurred at an aeration percentage of 90 % and a sharp decline in the production upon decrease of aeration.…”

Section: Resultsmentioning

confidence: 99%

Studies on the Effects of Bioprocess Parameters and Kinetics of Rhamnolipid Production by P. aeruginosa NITT 6L

Vanavil

Perumalsamy

Rao

2014

Chem.Biochem.Eng.Q.

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Biosurfactants are gaining popularity in recent times due to lower toxicity, biodegradability, environmental compatibility and activity in extreme conditions. An air isolate was isolated previously for biosurfactant production in our laboratory, and characterized and named as P. aeruginosa NITT 6L. The biosurfactant thus produced was characterized to be surface-active rhamnolipid. This paper presents the study of the influence of various bioprocess parameters such as agitation, aeration and inoculum volume on rhamnolipid production by the isolate. Kinetics of rhamnolipid production in optimized media and process conditions were investigated. The rhamnolipid production was found to be increased after nitrogen depletion during stationary phase. The maximum rhamnolipid concentration of about 7.65 g L -1 was achieved after 96 h. Logistic model was found to be satisfactory in fitting the microbial growth. Emulsification activity of the crude rhamnolipid extract with different hydrocarbons was studied. The crude extract of rhamnolipid reduced the surface tension of water from 71.4 to 27.5 mN m -1, and CMC was about 11 mg L -1 . Also, the usefulness of the extracted rhamnolipid produced under optimal conditions was investigated for remediation of crude oil contaminated soil. Soil washing with 0.3 % rhamnolipid removed about 71 % of crude oil present in sand samples within 24 h.

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Optimization of Surfactin Production by Bacillus subtilis Isolate BS5

Cited by 135 publications

References 40 publications

Characterization of Surfactin Produced by Bacillus subtilis Isolate BS5

Characterization of Surfactin Produced by Bacillus subtilis Isolate BS5

Production, Characterization, and Properties of Sophorolipids from the Yeast Candida bombicola using a Low-cost Fermentative Medium

Studies on the Effects of Bioprocess Parameters and Kinetics of Rhamnolipid Production by P. aeruginosa NITT 6L

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