Optimization of the cultivation medium for natamycin production byStreptomyces natalensis

Farid, Mohamed A.; El‐Enshasy, Hesham A.; El‐Diwany, Ahmed I.; El-Sayed, E.

doi:10.1002/1521-4028(200007)40:3<157::aid-jobm157>3.0.co;2-1

Cited by 61 publications

(30 citation statements)

References 12 publications

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“…Sodium chloride concentrations up to 4 g/L and KNO 3 up to 6 g/L had positive effects on bioactive metabolite production by NF15 (37). However, phosphate confers negative effects if the concentration is increased beyond 2 g/L (38, 39). …”

Section: Discussionmentioning

confidence: 99%

Biological Evaluation of Endophytic Fungus Chaetomium sp. NF15 of Justicia adhatoda L.: A Potential Candidate for Drug Discovery

Fatima

Mukhtar

ul-Haq

et al. 2016

Jundishapur J Microbiol

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BackgroundThe endophytes of medicinal plants, such as Justicia adhatoda L., represent a promising and largely underexplored domain that is considered as a repository of biologically active compounds.ObjectivesThe aim of present study was isolation, identification, and biological evaluation of endophytic fungi associated with the J. adhatoda L. plant for the production of antimicrobial, antioxidant, and cytotoxic compoundsMaterials and MethodsEndophytic fungi associated with the J. adhatoda L. plant were isolated from healthy plant parts and taxonomically characterized through morphological, microscopic, and 18S rDNA sequencing methods. The screening for bioactive metabolite production was achieved using ethyl acetate extracts, followed by the optimization of different parameters for maximum production of bioactive metabolites. Crude and partially purified extracts were used to determine the antimicrobial, antioxidant, and cytotoxic potentialResultsOut of six endophytic fungal isolates, Chaetomium sp. NF15 showed the most promising biological activity and was selected for detailed study. The crude ethyl acetate extract of NF15 isolate after cultivation under optimized culture conditions showed promising antimicrobial activity, with significant inhibition of the clinical isolates of Staphylococcus aureus (87%, n=42), Pseudomonas aeruginosa (> 85%, n = 41), and Candida albicans (62%, n = 24).ConclusionsThe present study confirms the notion of selecting endophytic fungi of medicinal plant Justicia for the bioassay-guided isolation of its bioactive compounds, and demonstrates that endophytic fungus Chaetomium sp. NF15 could be a potential source of bioactive metabolites

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Section: Discussionmentioning

confidence: 99%

Biological Evaluation of Endophytic Fungus Chaetomium sp. NF15 of Justicia adhatoda L.: A Potential Candidate for Drug Discovery

Fatima

Mukhtar

ul-Haq

et al. 2016

Jundishapur J Microbiol

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“…Further, the optimal levels of the suitable nitrogen source (0.1-1.5% w/v) for good yields of bioactive metabolites were also recorded [12]. To evaluate the effect of mineral salts, the optimized medium containing the superior carbon and nitrogen source was supplemented separately with mineral supplements such as KH 2 PO 4 , K 2 HPO 4 , NaCl, KCl, MgSO 4 .7H 2 O, FeSO 4 .7H 2 O, and MnCl 2 at a concentration of 0.05% (w/v) [13].…”

Section: Media Optimizationmentioning

confidence: 99%

Optimization, Isolation and Characterization of Bioactive Compounds From Streptomyces Lavendulocolor VHB-9

Bssn

Munaganti

Vijayalakshmi

et al. 2018

Asian J Pharm Clin Res

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Objectives: Optimization, isolation, and characterization of bioactive compounds from Streptomyces lavendulocolor VHB-9 isolated from granite mines of Mudigonda village of Khammam district of Telangana state. Methods:The potent strain was identified as S. lavendulocolor VHB-9 by polyphasic taxonomy. The influence of culture conditions on growth and bioactive compounds production was investigated. Purification of bioactive compounds was done using column chromatography. The structures of the compounds were elucidated on the basis of spectroscopic analysis including Fourier transform infrared, electron spray ionization mass spectrophotometry, 1 H nuclear magnetic resonance (NMR), and 13 C NMR. The antimicrobial activity of the compounds produced by the strain was tested against both Gram-positive and Gram-negative bacteria and fungi in terms of minimum inhibitory concentration.Results: Isolation and identification of two compounds, namely (2R, 3R)-2, 3-Butanediol (B1A), and nonadecanoic acid (B1B). Fraction B4 was isolated partially purified fraction and identified by the gas chromatography-mass spectrometry analysis. B1B compound exhibited the highest activity against Bacillus megaterium, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans when compared to B1A and B4 compounds.

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“…It is widely used in the food industry as a preservative on the surface of cheese, fruits and other non-sterile products, such as meat and sausages [61,17]. Pimaricin presents a broad spectrum of activity and any development of resistance has not been reported.…”

Section: Pimaricinmentioning

confidence: 99%

Smart Nanohydrogels for Controlled Release of Food Preservatives

González

2016

Antimicrobial Food Packaging

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Optimization of the cultivation medium for natamycin production byStreptomyces natalensis

Cited by 61 publications

References 12 publications

Biological Evaluation of Endophytic Fungus Chaetomium sp. NF15 of Justicia adhatoda L.: A Potential Candidate for Drug Discovery

Biological Evaluation of Endophytic Fungus Chaetomium sp. NF15 of Justicia adhatoda L.: A Potential Candidate for Drug Discovery

Optimization, Isolation and Characterization of Bioactive Compounds From Streptomyces Lavendulocolor VHB-9

Smart Nanohydrogels for Controlled Release of Food Preservatives

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