Optimization of the Duration and Dose of Photobiomodulation Therapy (660 nm Laser) for Spinal Cord Injury in Rats

Neshasteh-Riz, Ali; Ramezani, Fatemeh; Kookli, Keihan; Fazeli, Seyedalireza Moghaddas; Motamed, Ali; Nasirinezhad, Farinaaz; Janzadeh, Atousa; Hamblin, Michael R.; Asadi, Mohammadreza

doi:10.1089/photob.2022.0012

Cited by 9 publications

(9 citation statements)

References 62 publications

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“…[43][44][45] Previous studies have shown that PBMT improves peripheral 23 and central NP. 21 Reduction of inflammatory factors such as IL6, 46 iNOS, apoptotic factors, 23 and COX-2, 47 increase in GAD65 48 expression, antioxidants such as the amount of glutathione, 23 and modulation of energy metabolism (ATP), 46,47 are among the known mechanisms that lead to an alleviating of pain after nerve damage. It can help the absorption of reparative proteins 49 and improve the function of injected stem cells 50 in the path of nervous system recovery.…”

Section: Discussionmentioning

confidence: 99%

“…The nerve regeneration, antiinflammatory, and analgesic effects of PBMT are well understood. [19][20][21] Although, the exact PBMT mechanism of action is not known. PBMT has no cutting or thermal mechanism but has a photochemical effect, which means that light is absorbed and causes chemical changes.…”

Section: Introductionmentioning

confidence: 99%

“…PBMT has no cutting or thermal mechanism but has a photochemical effect, which means that light is absorbed and causes chemical changes. 19,21,22 Our previous studies in the animal phase showed that PBMT could improve the threshold of NP following the CCI model. 23,24 The question is that although PNS damage causes changes in epigenetic levels, PBMT, which is an effective treatment for pain control, can control this mechanism?…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of epigenetic (HDAC, DNMT) and pain (Gad65, TGF) factors following photobiomodulation therapy in a neuropathic pain model

Behroozi

Kookli

Ghadaksaz

et al. 2023

Photochem & Photobiology

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Photobiomodulation therapy (PBMT) is converted to the most common analgesic treatment before the whole mechanism is yet to be discovered. This study for the first time was designed to investigate alternations of epigenetic factors after pain and PBMT. The CCI model was chosen to induce pain. Pain evaluation tests including plantar, acetone, von Frey, and pinch were done weekly. Then spinal cord tissue was isolated for evaluating mRNA expression of DNMT3a, HDAC1, and NRSF using RT‐qPCR method, and protein expression factors of HDAC2 and DNMT3a using western blotting. GAD65 and TGF‐β proteins were assessed by the IHC method. PBMT increased the pain threshold up to the point where it roughly met the pain threshold of the control group. After three weeks of treatment, both PBMT protocols demonstrated a reduction in allodynia and hyperalgesia. While some molecules, such as TGF‐β and Gad65, increased following PBMT, we observed no inhibition of NRSF, HDAC1, and DNMT3a expression despite implementing two different protocols.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of epigenetic (HDAC, DNMT) and pain (Gad65, TGF) factors following photobiomodulation therapy in a neuropathic pain model

Behroozi

Kookli

Ghadaksaz

et al. 2023

Photochem & Photobiology

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“…Nine points around the surgical incision were transcutaneous irradiated between 10 and 12 a.m. as follows: three points on the center level of the injury site, three points caudally, and three points rostral. Total irradiation time was 90 and 117 seconds (10 and 13 seconds for each point) (29,30). Laser calibration was done using a power meter (FieldMaxII-TO, Coherent Inc).…”

Section: Surgerymentioning

confidence: 99%

“…Post-operative care included antibiotic prescription (Penicillin 0.8mg/100g, i.p) and bladder emptying (twice daily until full recovery). Animals were followed for 7 weeks after surgery (29).…”

Section: Surgerymentioning

confidence: 99%

Evaluation of Photobiomodulation Therapy (117 and 90 seconds) on Pain, Regeneration, and Epigenetic Factors (HDAC 2, DNMT3a) Expression Following Spinal Cord Injury in A Rat Model

MotamedNejad

Behroozi

Kookli

et al. 2023

Preprint

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Background Photobiomodulation therapy (PBM), due to its anti-inflammatory, analgesic effects, and most importantly as a non-invasive procedure, has currently gained a special setting in pain relief and the treatment of Spinal cord injuries (SCI). However, the mechanism of action of the PBM is not yet completely understood. Methods In this study, SCI is induced by an aneurysm clip. Adult male rats were divided into 4 groups: Control, SCI, SCI + PBMT 90 seconds, and SCI + PBMT 117 seconds. After 7 weeks, hyperalgesia, allodynia, and functional recovery were assessed. Fibroblasts were counted after H&E staining. The expression of HDAC2, DNMT3a, GAD65, and GFAP after 4 weeks of daily PBMT (90 and 117 s) was probed by western blotting. Results Both PBMTs (90 & 117 seconds) significantly improved the pain and ability to move and fibroblast invasion was reduced. SCI + PBMT 90s, increased GAD65, HDAC2, and DNMT3a expression. However,PBMT 117s decreased GFAP, HDAC2, and DNMT3a. Conclusion PBMT 90 and 117 s improved the pain, and functional recovery equally. Yet, the mechanism of action appears to change with different durations of radiation.

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The effect of intra spinal administration of cerium oxide nanoparticles on central pain mechanism: An experimental study

Mostaar,

Behroozi,

MotamedNezhad

et al. 2024

J Bioenerg Biomembr

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Optimization of the Duration and Dose of Photobiomodulation Therapy (660 nm Laser) for Spinal Cord Injury in Rats

Cited by 9 publications

References 62 publications

Evaluation of epigenetic (HDAC, DNMT) and pain (Gad65, TGF) factors following photobiomodulation therapy in a neuropathic pain model

Evaluation of epigenetic (HDAC, DNMT) and pain (Gad65, TGF) factors following photobiomodulation therapy in a neuropathic pain model

Evaluation of Photobiomodulation Therapy (117 and 90 seconds) on Pain, Regeneration, and Epigenetic Factors (HDAC 2, DNMT3a) Expression Following Spinal Cord Injury in A Rat Model

The effect of intra spinal administration of cerium oxide nanoparticles on central pain mechanism: An experimental study

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