Optimization of the Multienzyme-Assisted Extraction Procedure of Bioactive Compounds Extracts from Common Buckwheat (Fagopyrum esculentum M.) and Evaluation of Obtained Extracts

Abstract

Optimization of the extraction procedure using a multienzymes cocktail for common buckwheat (Fagopyrum esculentum M.) is important due to the yield, fermentable sugars, oligosaccharides and bioactive compounds for creating higher added value products. This study was undertaken to find out the optimum multienzymes-water extraction on yield and total phenolic compounds for common Buckwheat using response surface methodology (RSM). Three independent variables, time (2, 13, and 24 h), temperature (60 °C, 70 °C, 80 °C), and non-starch polysaccharide (NSP) enzymes mixture (0.10, 0.55, and 1.00 mL), were analyzed to optimize the response variables. NSP hydrolyzing enzymes, cellulase, xylanase, and β-glucanase, were produced by Trichoderma reesei. Estimated optimum conditions for F. esculentum were found: time—2 h, temperature—65 °C, and cellulase activity—8.6 CellG5 Units/mL. Different optimization run samples were collected and lyophilized for further analysis until the hydrophilic property using the water contact angle methodology and rutin content using HPLC was determined. Results indicated NSP enzymes activity did not differ between water contact angles after 13 h of enzymatic water extraction. However, longer fermentation time (24 h) decreased static water contact angle by approximately 3–7° for lyophilized water extract and 2–7° for solid fraction after fermentation. It implies enzymatic hydrolysis during water extraction increased hydrophilic properties in solid fraction and decreased hydrophilicity in water fraction due to the enzymes cleaved glycosidic bonds releasing water-soluble compounds.

Optimization of Enzyme-Assisted Extraction of Bioactive Compounds from Sea Buckthorn (Hippophae rhamnoides L.) Leaves: Evaluation of Mixed-Culture Fermentation

“…SBL biomass is rich in bioactive compounds but is usually regarded as waste, although obtained EAE extracts could be interesting in various sectors. The key benefits of EAE are cost-effectiveness, environmental friendliness, and the ability to release phenolics from their insoluble bonds as more bioactive components [ 14 , 19 , 39 ]. Multiple authors have suggested RSM and CCD to assess and optimize enzymatic hydrolysis factors such as temperature, time, enzyme concentration, and pH [ 19 , 40 , 41 ].…”

“…EAE was carried out as described by Streimikyte et al [ 19 ], with minor adjustments. SBL samples were extracted using hot water EAE at a 1:20 ( w / v ) ratio and mixed to obtain a homogeneous suspension.…”

“…For instance, EAE has the ability to extract pectin from waste and by-products by enhancing the permeability of the plant cell wall [ 13 , 17 , 18 ]. By implementing this technique, it is possible to increase the yields of extracts within a shorter extraction time and reduce solvent use while simultaneously enhancing the concentration of bioactive compounds such as various phenolic compounds, including flavonoids and anthocyanidins [ 19 ]. This approach also minimizes production costs and time requirements while ensuring that environmental sustainability is prioritized throughout the process [ 15 ].…”

The Biochemical Alteration of Enzymatically Hydrolysed and Spontaneously Fermented Oat Flour and Its Impact on Pathogenic Bacteria

“…Enzyme-assisted extraction was performed with slight modifications as described by Streimikyte et al [ 35 ]. Two different batches were analysed.…”

Antimicrobial Activities against Opportunistic Pathogenic Bacteria Using Green Synthesized Silver Nanoparticles in Plant and Lichen Enzyme-Assisted Extracts

“…An amount of 20 g of raw material of each plant is weighed (exact mass of ground material), poured over 120 g of distilled, and added to the 0.15% ( w / w ) the multienzyme non-starch polysaccharide (NSP) cocktail (JSC “Baltijos Enzimai”, Lithuania) as described by Streimikyte et al [ 24 ]. The main enzyme cocktail component is cellulase.…”