Optimization of Thin-layer Chromatography and High-Performance Liquid Chromatographic Method for <i>Piper guineense</i> Extracts

Mgbeahuruike, Eunice Ego; Vuorela, Heikki; Yrjönen, Teijo; Holm, Yvonne

doi:10.1177/1934578x1801300109

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…Piper guineense Schumach & Thonn, also known as African guinea pepper or African black pepper, is a medicinal plant that has a wide application in traditional medicine and is therefore of great ethnobotanical interest [7,8,9]. It is a perennial climbing vine reaching heights of 20 m. African black pepper has a pungent aroma and the fruits, leaves and roots are used for the preparation of herbal remedies for the treatment of infectious diseases [10,11].…”

Section: Introductionmentioning

confidence: 99%

Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

et al. 2018

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Piper guineense is a food and medicinal plant commonly used to treat infectious diseases in West-African traditional medicine. In a bid to identify new antibacterial compounds due to bacterial resistance to antibiotics, twelve extracts of P. guineense fruits and leaves, obtained by sequential extraction, as well as the piperine and piperlongumine commercial compounds were evaluated for antibacterial activity against human pathogenic bacteria. HPLC-DAD and UHPLC/Q-TOF MS analysis were conducted to characterize and identify the compounds present in the extracts with promising antibacterial activity. The extracts, with the exception of the hot water decoctions and macerations, contained piperamide alkaloids as their main constituents. Piperine, dihydropiperine, piperylin, dihydropiperylin or piperlonguminine, dihydropiperlonguminine, wisanine, dihydrowisanine and derivatives of piperine and piperidine were identified in a hexane extract of the leaf. In addition, some new piperamide alkaloids were identified, such as a piperine and a piperidine alkaloid derivative and two unknown piperamide alkaloids. To the best of our knowledge, there are no piperamides reported in the literature with similar UVλ absorption maxima and masses. A piperamide alkaloid-rich hexane leaf extract recorded the lowest MIC of 19 µg/mL against Sarcina sp. and gave promising growth inhibitory effects against S. aureus and E. aerogenes as well, inhibiting the growth of both bacteria with a MIC of 78 µg/mL. Moreover, this is the first report of the antibacterial activity of P. guineense extracts against Sarcina sp. and E. aerogenes. Marked growth inhibition was also obtained for chloroform extracts of the leaves and fruits against P. aeruginosa with a MIC value of 78 µg/mL. Piperine and piperlongumine were active against E. aerogenes, S. aureus, E. coli, S. enterica, P. mirabilis and B. cereus with MIC values ranging from 39–1250 µg/mL. Notably, the water extracts, which were almost devoid of piperamide alkaloids, were not active against the bacterial strains. Our results demonstrate that P. guineense contains antibacterial alkaloids that could be relevant for the discovery of new natural antibiotics.

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Section: Introductionmentioning

confidence: 99%

Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

et al. 2018

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“…An attempt was made to find the presence of major functional groups of some known phytoconstituents. 6 X= Distance traveled by sample Y= Distance traveled by solvent…”

Section: Calculation Of Rf Valuesmentioning

confidence: 99%

Antitussive Activity of Alcoholic Extract of Piper longum Linn.

Bedse,

Nalawade,

Kamandar

et al. 2023

IJPQA

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The l-phellandrene and caryophyllene found in Piper longum fruits are effective against a wide range of infections, including stomachic, bronchitis, spleen, cough, tumor, and asthma. This study reports preparing and characterizing alcohol-soluble extract of P. longum L. fruit powder. FTIR spectroscopy and TLC were used to characterize an alcoholic extract of P. longum. P. longum L. alcohol soluble extract was tested for its anti-tussive efficacy using the standard mouse cough model caused by ammonia liquor. Cough frequency was inhibited by 49.51% (Marketed formulation), 50.84% (Extract 100 mg/kg), 62.71% (Extract 200 mg/kg). Statistically significant (p <0.001) difference was observed in %inhibition of cough frequency of both doses of extract when compared with the marketed formulation. This study reveals an anti-tussive activity of alcohol soluble extract of P. longum L.

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Overview of High-Performance Thin Layer Chromatography and its Applications in Pesticide Analysis

Kandasamy

Muruganantham

Krishnaswami

et al. 2023

CAC

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In recent decades, increased demand for food has been caused by a rapid rise in the human population, which triggers agricultural intensification. To resist undesired pests from infecting crops, farmers widely utilize pesticides to improve agricultural production during the pre-harvest period. Despite the fact that pesticides cause a number of health risks, there is insufficient monitoring of these toxins. Therefore, it is important to develop a specific, accurate, and efficient method for determining the pesticides in varied samples in order to safeguard health against potential risks. Due to the lower concentrations of active compounds and their diversity of availability, it is challenging to detect pesticide residues in different samples. In this case, to effectively separate, identify, and accurately quantify pesticides at low concentrations in a variety of samples, a reliable analytical methodology is needed. Recently, the application of high-performance thin layer chromatography (HPTLC) offers a wider scope with excellent separation, identification, and quantitative/qualitative determination in pesticide analysis. In spite of their extremely low quantities, pesticide residues can be accurately and precisely identified using HPTLC. HPTLC has a number of benefits, such as easy sample preparation, automation, densitometry, and hyphenation, and is particularly well suited for identification and detection. Concerning this, the proposed review paper provides an overview of stationary phases, mobile phases, sample applicators, visualization, derivatization, and detection of HPTLC utilized for the identification and detection of pesticide residues in agriculture and environmental samples.

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Optimization of Thin-layer Chromatography and High-Performance Liquid Chromatographic Method for Piper guineense Extracts

Cited by 4 publications

References 19 publications

Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Antitussive Activity of Alcoholic Extract of Piper longum Linn.

Overview of High-Performance Thin Layer Chromatography and its Applications in Pesticide Analysis

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