Optimization of transient GUS expression of Agrobacterium-mediated transformation in Dierama erectum Hilliard using sonication and Agrobacterium

Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea

“…However, seeds produced from A. tumefaciens plus Km-treated pollen exhibited significantly improved Km-resistance (86.7% for T. violacea and 95.6% for A. nelsonii) and GUS-positive (97.5% for T. violacea and 100% in A. nelsonii) seedlings (Table 1 and Figure 1G, H). Effectiveness of Km selection and GUS expression for production of putative transgenic plants in different methods has been reported for other plant species (Bino et al 1987, Twell et al 1990, Baskaran and Dasgupta 2012, Baskaran et al 2016, Koetle et al 2017, Souza et al 2017. GUS-positive seedlings were successfully acclimatized with 1:1 (v/v) vermiculite:soil mixture in the greenhouse.…”

In vitro organogenesis in tomato cultivars is enhanced by gas exchange and application of ultrasound

“…Also causes changes in the less stable, extended form of nucleolar chromatin (Elsner and Lindblad, 1989). In in vitro cultivation, ultrasound is used to obtain the largest number of branches in the genetic transformation procedure known as Sonication-assisted Agrobacterium-mediated genetic transformation (SAAT) (Koetle et al, 2017). Ananthakrishnan et al (2007) stimulated multiple shoot regeneration from recalcitrant cotyledon explants from seeds before germination of commercial squash (Cucurbita pepo L.) that was the first demonstration that ultrasound exerts morphogenic influence by surface removal, however the technique has been caused hyper hydratation of the explants.…”