Optimization of Trehalose Lypids Extraction – Metabolites of Rhodococcus erythropolis Au-1

Koretska, Nataliia; Midyana, G. G.; Karpenko, Elena

doi:10.20535/ibb.2018.2.4.148935

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…У роботі [15] встановлено, що штам R. erythropolis Au-1 з Української колекції мікроорганізмів Інституту мікробіології і вірусології ім. Д.К.…”

Section: біосинтез поверхнево-активних речовин на гідрофобних субстратахunclassified

“…Корецька зі співавторами [15] показали, що R. erythropolis Au-1 синтезує асоційовані з клітинами ПАР не тільки на гідрофобних субстратах (вуглеводні, олії, див. табл.…”

Section: утворення поверхнево-активних речовин на гідрофільних субстратахunclassified

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Biosynthesis of Surfactants by Actinobacteria of Rhodococcus Genus

Пирог¹,

Heichenko²,

Shevchuk³

et al. 2020

Mikrobiol. Z.

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Section: біосинтез поверхнево-активних речовин на гідрофобних субстратахunclassified

Section: утворення поверхнево-активних речовин на гідрофільних субстратахunclassified

Biosynthesis of Surfactants by Actinobacteria of Rhodococcus Genus

Пирог¹,

Heichenko²,

Shevchuk³

et al. 2020

Mikrobiol. Z.

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“…Generally they are less toxic and more biodegradable than synthetic surfactants (Jahan et al, 2020;Mulligan et al, 2014). Тhe biosurfactants increase permeability of cell membranes, so enhance the effect of various biological active compounds (Sotirova et al, 2012;Koretska et al, 2020;Yaremkevych et al, 2020). Therefore, much attention is paid to the use of biosurfactants in various industries, mainly in petroleum, food, cosmetics, pharmaceuticals, bioremediation and agriculture (Ceresa et al, 2021;Markande et al, 2021;da Silva et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN

Semeniuk¹,

Koretska

Кочубей³

et al. 2022

J microb biotech food sci

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Practically important metabolites of Rhodococcus erythropolis Au-1 strain were obtained: trehalose lipids, exopolysaccharide and cellular polymer – polyhydroxyalkanoate. The structures of the products were established by the methods of TLC, IR and UV-Vis spectroscopy. The purification of trehalose lipids from crude extract was carried out using silica gel column chromatography, one of the trehalose lipids fractions was identified as a trehalose dimycolate. The presence of (NН) group was detected in the exopolysaccharide. For the first time, a biodegradable polymer was isolated from bacteria cells of the strain R. erythropolis Au-1, іt was identified as the polyhydroxyalkanoate. The physico-chemical properties of the metabolites of the strain R. erythropolis Au-1 were established: the surface tension of trehalose lipids fractions was 30,5-43,2 mN/m; the emulsification activity of the exopolysaccharide solutions (1-10 g/L) with vaseline oil was 42-58%. The differential thermal analysis was used to define starting temperatures of degradation of trehalose dimycolate (130 °C), exopolysaccharide (180 °C) and polyhydroxyalkanoate (164 °C, melting point 42oC). The obtained results expand the possibilities of practical application of trehalose lipids, exopolysaccharide and polyhydroxyalkanoate of the strain R. erythropolis Au-1.

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“…Враховуючи принцип лінійності вільних енергій (ЛВЕ), дані з розчинності таких речовин, як нітронафталін, фулерен [9,10] та інші були кількісно пов'язані з властивостями розчинників за допомогою багатопараметрових лінійних рівнянь [11]. Тривалий час такі дослідження проводили тільки для індивідуальних сполук, а також застосовували для вугільних смол [12], але рідко для аналізу результатів екстракції біоПАР [13][14][15]. У наших дослідженнях ми маємо справу з високомолекулярними речовинами природного походження.…”

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Application of Extraction Method for Isolation of Biogenic Surface-Active Rhamnolipids

Карпенко¹,

Midyana²,

Карпенко³

et al. 2020

Proc. Shevchenko Sci. Soc. Chem. Sci.

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The priority task of modern biotechnology is development of the rational technologies for the microbial synthesis of practically important products. Among these products, a significant place belongs to surfactants (biosurfactants), which are widely used in many sectors of the economy. The most problematic stage of the biosurfactants production is isolation from the post fermentative cultural liquid of bacteria-producers. Improving the efficiency of the biosurfactants production is highly dependent on rational approaches to the target products isolation. In this regard, there is an increasing need for rational, scientifically substantiated methods for their isolation and purification. Therefore, the aim of the presented work was to determine the optimal extractants for the isolation of rhamnolipid surfactants – metabolites of bacteria of Pseudomonas sp. PS-17 strain. For this purpose, the extraction process of rhamnolipids from the post fermentative cultural liquid supernatant has been investigated. The optimal extractants were selected among 13 organic solvents of different nature. Processing of the obtained experimental data by the method of multi-parameter equations of linearity of free energies (modified Koppel-Palm equation) made it possible to establish the relationship between the physicochemical properties of the extractants and amounts of the biosurfactants which were isolated from cultural liquid supernatant. It was shown that the data on the rhamnolipids extraction are adequately associated with the physicochemical characteristics of the solvents using a six-parameter linear equation. It was determined that the polarizability and molar volume are the main properties of solvents that affect the extraction process. The best extractants for the rhamnolipids isolation from cultural liquid supernatant of the Pseudomonas sp. PS-17 strain are the ethers. It can be explained by the presence of a lone pair of the electrons of oxygen in its molecule. The obtained results of the study are of scientific interest for isolation of the important and perspective biotechnological products – surface-active substances.

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Optimization of Trehalose Lypids Extraction – Metabolites of Rhodococcus erythropolis Au-1

Cited by 4 publications

References 12 publications

Biosynthesis of Surfactants by Actinobacteria of Rhodococcus Genus

Biosynthesis of Surfactants by Actinobacteria of Rhodococcus Genus

BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN

Application of Extraction Method for Isolation of Biogenic Surface-Active Rhamnolipids

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