Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

Gendre, Dylan A J; Ameti, Edis; Karenovicŝ, Wolfram; Perriraz-Mayer, Nadja; Triponez, Frédéric; Serre‐Beinier, Véronique

doi:10.18632/oncotarget.28134

Cited by 16 publications

(16 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This phase of exponential growth aligns with observations made in the standard BME2 dome 3D-culture method, where spheroid size increased steadily without disaggregation between day 3 to day 15. 34 However, as we extended the incubation period into the 3rd week in our agarose microwells, a reduction in size was observed. Specifically, F231 PDOs decreased from approximately 41 000 μm 2 to 37 500 μm 2 , while F671 PDOs decreased form ∼60 000 μm 2 to ∼57 000 μm 2 .…”

Section: Resultsmentioning

confidence: 91%

Deciphering fibroblast-induced drug resistance in non-small cell lung carcinoma through patient-derived organoids in agarose microwells

Luan,

Pulido,

Isagirre

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 91%

Deciphering fibroblast-induced drug resistance in non-small cell lung carcinoma through patient-derived organoids in agarose microwells

Luan,

Pulido,

Isagirre

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

“…We investigated whether loss of ADAR2 affected the sensitivity of RN5 and Mero95 cells to pemetrexed treatment in vitro . High pemetrexed doses are necessary in 3D mesothelioma models compared to the ones usually used in 2D (55), but PM spheroids better represent biological complexity of the tumor in vivo (56, 57). Upon pemetrexed treatment, size and cell viability RN5 ADAR2 KD spheroids were reduced (Fig.6A).…”

Section: Resultsmentioning

confidence: 99%

Heterogeneity of RNA editing in mesothelioma and how RNA editing enzyme ADAR2 affects mesothelioma cell growth, response to chemotherapy and tumor microenvironment

Hariharan

Rehrauer

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

We previously observed increased levels of adenosine-deaminase-acting-on-dsRNA (Adar)-dependent RNA editing during mesothelioma development in mice exposed to asbestos. The aim of this study was to characterize and assess the role of ADAR-dependent RNA editing in mesothelioma. Tumors and mesothelioma primary cultures have higher ADAR-mediated RNA editing compared to mesothelial cells. Unsupervised clustering of editing in different genomic regions revealed heterogeneity between tumor samples as well as mesothelioma primary cultures. ADAR2 expression levels are higher in BRCA1-associated protein 1 wild-type tumors, with corresponding changes in RNA editing in transcripts and 3-UTR. ADAR2 knockdown and rescue models indicated a role in cell proliferation, altered cell cycle, increased sensitivity to antifolate treatment and type-1 interferon signaling upregulation, leading to changes in the microenvironment in vivo. Our data indicate that RNA editing contributes to mesothelioma heterogeneity and highlights an important role of ADAR2 not only in growth regulation in mesothelioma but also chemotherapy response, in addition to regulating inflammatory response downstream of sensing nucleic acid structures.

show abstract

“…We investigated whether loss of ADAR2 affected the sensitivity of RN5 and Mero95 cells to pemetrexed treatment in vitro . High pemetrexed doses are necessary in 3D mesothelioma models compared to the ones usually used in 2D [ 60 ], but PM spheroids better represent biological complexity of the tumor in vivo [ 61 , 62 ]. Upon pemetrexed treatment size and cell viability, RN5 ADAR2 KD spheroids were reduced (Fig.…”

Section: Resultsmentioning

confidence: 99%

Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment

Hariharan

Rehrauer

et al. 2022

Molecular Oncology

View full text Add to dashboard Cite

We previously observed increased levels of adenosine-deaminase-acting-on-dsRNA (Adar)-dependent RNA editing during mesothelioma development in mice exposed to asbestos. The aim of this study was to characterize and assess the role of ADAR-dependent RNA editing in mesothelioma. We found that tumors and mesothelioma primary cultures have higher ADAR-mediated RNA editing compared to mesothelial cells. Unsupervised clustering of editing in different genomic regions revealed heterogeneity between tumor samples as well as mesothelioma primary cultures. ADAR2 expression levels are higher in BRCA1-associated protein 1 wild-type tumors, with corresponding changes in RNA editing in transcripts and 3'UTR. ADAR2 knockdown and rescue models indicated a role in cell proliferation, altered cell cycle, increased sensitivity to antifolate treatment, and type-1 interferon signaling upregulation, leading to changes in the microenvironment in vivo. Our data indicate that RNA editing contributes to mesothelioma heterogeneity and highlights an important role of ADAR2 not only in growth regulation in mesothelioma but also in chemotherapy response, in addition to regulating inflammatory response downstream of sensing nucleic acid structures.

show abstract

Optimization of tumor spheroid model in mesothelioma and lung cancers and anti-cancer drug testing in H2052/484 spheroids

Cited by 16 publications

References 47 publications

Deciphering fibroblast-induced drug resistance in non-small cell lung carcinoma through patient-derived organoids in agarose microwells

Deciphering fibroblast-induced drug resistance in non-small cell lung carcinoma through patient-derived organoids in agarose microwells

Heterogeneity of RNA editing in mesothelioma and how RNA editing enzyme ADAR2 affects mesothelioma cell growth, response to chemotherapy and tumor microenvironment

Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment

Contact Info

Product

Resources

About