Optimization of Xylanase Production from<i> Aspergillus foetidus </i>in Soybean Residue

Cunha, Luana; Martarello, Raquel; Souza, Paula Monteiro; Freitas, Marcela Medeiros de; Barros, Kleber Vânio Gomes; Filho, Edivaldo Ximenes Ferreira; Homem-de-Mello, Maurício; Magalhães, Pérola Oliveira

doi:10.1155/2018/6597017

Cited by 42 publications

(19 citation statements)

References 22 publications

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“…Xylanase is widely used in industrial applications due to its potential application value. However, due to the high cost and low enzyme activity, the application of xylanase is limited . The reduction in the cost of xylanase production is mainly through increasing the expression of xylanase and finding a cheaper and convenient fermentation medium .…”

Section: Discussionmentioning

confidence: 99%

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The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

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Xylanase is a member of an important family of enzymes that has been used in many biotechnological processes. However, the overall cost of enzyme production has been the main problem in the industrial application of enzymes. To obtain maximum xylanase production, statistical approaches based on the Plackett-Burman design and response surface methodology were employed. The results of the statistical analyses demonstrated that the optimal conditions for increased xylanase production were the following: inoculum size, 3.8%; maize meal, 4.5%; histidine, 0.6%; methanol, 1%; culture volume, 20%; bean pulp, 30 g L −1 ; and Tween-80, 0.8%; and pH 5.0. Verification of the optimization demonstrated that 3273 U mL −1 xylanase was observed under the optimal conditions in shake flask experiments. SDS-PAGE results showed that the size of xylanase protein was about 23 kDa. The results showed that the xylanase produced by fermentation came from Aspergillus Niger by MALDI-TOF-MS. The optimized medium resulted in 2.1-and 1.4-fold higher the activity of xylanase compared with the unoptimized medium (the main nutrients are maize meal and bean pulp) and laboratory medium (the main nutrients are yeast extract and peptone), respectively.The optimization of fermentation conditions is an effective means to reduce production cost and improve xylanase activity. K E Y W O R D Smethodology optimization, Plackett-Burman, xylanase Abbreviations: PBS, phosphate-buffered saline; RSM, response surface method.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

“…Agricultural production is a suitable basic substrate for the economic production of xylanases. Wheat bran, corncob, and oat bran have been reported as the most commonly used substrates for xylanase production .…”

Section: Discussionmentioning

confidence: 99%

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

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“…According to Cunha et al (2018), the medium used for xylanase production was composed of (%): 0.4 peptone, 0.4 yeast extract, 0.2 KH 2 PO 4 , 0.8 (NH 4 ) 2 SO 4 , 0.25 MgSO 4 .7H 2 O, 2% substrate at pH 7.0. A 50 ml of fermentation medium into a 250-ml Erlenmeyer flasks was inoculated by two disks in diameter from the fungal strain and incubated on rotary shaker at 150 rpm, on 30°C for 7 days.…”

Section: Production Mediummentioning

confidence: 99%

Statistical optimization of xylanase production, using different agricultural wastes by Aspergillus oryzae MN894021, as a biological control of faba bean root diseases

Atalla

Ahmed

Awad

et al. 2020

Egypt J Biol Pest Control

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Background Xylanase enzyme plays an important role in nature as being a part of protecting the environment from pollution. It has also various industrial applications. Main body of abstract Marine fungal isolate was recovered from red sea water at Sharm El-Sheikh province, Egypt, and tested for xylanase activity, using different agricultural wastes as a substrate. It was found that rice straw was the best substrate for xylanase production (0.37 U/ml). Thus, it was subjected for identification by 18S rDNA gene. The phylogenetic analysis results indicated that this fungal isolate belonging to Aspergillus species with a similarity of 99% and named as A. oryzae SS_RS-SH (MN894021). The regular two-level factorial design was used to optimize the important medium components, which significantly affected the xylanase production. The model in equation suggested optimal conditions of 2% of rice straw, 8 g/l of yeast extract, 4 g/l of (NH4)2SO4, 2 g/l K2HPO4, and 2.5 g/l MgSO4.7H2O for a maximum xylanase yield. The antifungal activity of crude xylanase on mycelial growth of some pathogenic fungi isolated from different hosts was investigated. The results showed that xylanase T1 had a potent antifungal activity than control. Greenhouse experiments indicated that all treatments with xylanase at different concentrations significantly decreased infection occurrence of beans, which have been effectively infected with root rot pathogens, compared to unprocessed control treatments. Short conclusion Xylanase yield increased 2.43-folds than initial screening. The xylanase had a potential antifungal activity both in vitro and under greenhouse conditions. The outcome of this study ensured that this fungal strain could be used as biological control for plant disease.

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“…The production of xylanase by Aspergillus foetidus from soybean okara was studied by Cunha et al 80 . The authors used enzymatic hydrolysis to produce the enzyme and optimized the process using statistical analysis to assess the ways in which parameters such as pH and temperature affected the production of xylanase.…”

Section: Resultsmentioning

confidence: 99%

Soybean‐based concept biorefinery

Biazatti

Miranda

2021

Biofuels Bioprod Bioref

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Soybean is an important commodity. Its processing generates a great variety of important commercial products and processable residues. Using the concept of biorefinery, it is possible to integrate different technologies and increase the value added by the whole process. In this work, we conducted an oriented review. First, a base process was chosen, which would produce edible soybean oil, soy protein, and soy protein isolate. From this base process, we listed the residues and made a review of the processing technologies to convert them into valuable products. We elaborated a process network for each residue, linking the intermediary products to their processing technologies and, subsequently, the commercial value‐added products. Based on a price and market evaluation, the result was an integrated conceptual biorefinery process for soybean processing, mostly considering the Brazilian scenario. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Optimization of Xylanase Production from Aspergillus foetidus in Soybean Residue

Cited by 42 publications

References 22 publications

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Statistical optimization of xylanase production, using different agricultural wastes by Aspergillus oryzae MN894021, as a biological control of faba bean root diseases

Soybean‐based concept biorefinery

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