2022
DOI: 10.3390/ph15101230
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Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging

Abstract: The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were crea… Show more

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Cited by 5 publications
(2 citation statements)
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“…Mass spectrometry imaging is employed to construct molecular atlases for zebrafish larvae, facilitating the examination of specific molecules in different tissues. This technology can identify e-cigarette aerosol constituents and toxicants within zebrafish, aiding in the analysis of potential chemical exposures [ 151 , 152 ]. Integrating this technology with complementary assays offers a powerful approach to examining complex compounds in electronic cigarettes and understanding their health effects [ 153 ].…”
Section: Pushing the Boundaries Of E-cigarette Research With Zebrafis...mentioning
confidence: 99%
“…Mass spectrometry imaging is employed to construct molecular atlases for zebrafish larvae, facilitating the examination of specific molecules in different tissues. This technology can identify e-cigarette aerosol constituents and toxicants within zebrafish, aiding in the analysis of potential chemical exposures [ 151 , 152 ]. Integrating this technology with complementary assays offers a powerful approach to examining complex compounds in electronic cigarettes and understanding their health effects [ 153 ].…”
Section: Pushing the Boundaries Of E-cigarette Research With Zebrafis...mentioning
confidence: 99%
“…Organ toxicity after long-term or high-dose exposure to a drug can be tested on vertebrate models, such as rats, mice, minipigs, rabbits, and monkeys. In addition to these model animals, zebrafish, and its larvae or embryos are another important vertebrate model in genetics and toxicology (Figure 8b) [96,97]. Shao et al examined the distribution and metabolism of diazepam (DZP) and its chlorination disinfection byproduct, 2-methylamino-5-chlorobenzophenone (MACB), in zebrafish by DESI-MSI.…”
Section: Safety and Toxicity Evaluationmentioning
confidence: 99%