Optimization, purification, and biochemical characterization of thermoalkaliphilic lipase from a novel Geobacillus stearothermophilus FMR12 for detergent formulations

Abol-Fotouh, Deyaa; Al-Hagar, Ola E.A.; Hassan, Mohamed A.

doi:10.1016/j.ijbiomac.2021.03.111

Cited by 50 publications

(32 citation statements)

References 36 publications

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“…Other studies also found that the glucose supplementation to the basal medium acts as an inhibitor of lipase due to the catabolic repression. The effect of various nitrogen sources including yeast extract [ 26 ], ammonium salts, sodium nitrate, urea, peptone, and tryptone [ 27 – 30 ] has also been studied previously.…”

Section: Resultsmentioning

confidence: 99%

Optimization and characterization of alkaliphilic lipase from a novel Bacillus cereus NC7401 strain isolated from diesel fuel polluted soil

et al. 2022

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Five Bacillus cereus strains including B. cereus AVP12, B. cereus NC7401, B. cereus BDBCO1, B. cereus JF70 and B. specie JL47 isolated from the diesel fuel polluted soil adhered to the roots of Tagetes minuta were screened for lipase production with phenol red agar method. B. cereus NC7401 strain successfully expressing and secreting lipase with maximal lipolytic activity was subjected to a submerged fermentation process with five different carbon (starch, glucose, maltose, fructose, and lactose) and five different nitrogen (tryptone, ammonium nitrate, peptone, urea, yeast extract) sources to produce lipase enzyme. Maximum enzyme activity was found with starch (30.6 UmL-1), maltose (40 UmL-1), and tryptone (38.6 UmL-1), and the lipases produced using these sources were named lipase A, B, and C respectively. The total protein content of 8.56, 8.86, and 2.75 μg mL-1 were obtained from B. cereus NC7401 cultured using starch, maltose, and tryptone respectively. Lipase was stable between temperature range 30–80°C and pH 5–10 whereas optimally active at 55°C and pH 8.0. The enzyme was relatively stable for 10 days at 4°C and its optimum reaction time with the substrate was 30 minutes. It was tolerant to 1.5% (v/v) methanol as an organic solvent, 1.5% (v/v) Triton X-100 as a media additive and 1.5% (w/v) Ni2+ as a metal ion. SDS, n-hexane, and Ag+ inhibited lipolytic activity. Oil stains were removed from cotton fabric which showed oil removal efficiency enhancement in the presence of a lipase. Fat hydrolysis of 20, 24, and 30% was achieved following 6 hours of incubation of the fat particles with lipase A, B, and C respectively at a concentration of 20 mg mL-1. To as best of our knowledge, this study on lipases extracted from bacteria of Azad Kashmir, Pakistan origin has never been reported before.

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Section: Resultsmentioning

confidence: 99%

Optimization and characterization of alkaliphilic lipase from a novel Bacillus cereus NC7401 strain isolated from diesel fuel polluted soil

et al. 2022

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“…L2 [ 16 , 32 ]. In addition, when TLL was fermented in a 50 L fermenter in industry, its enzyme activity in the supernatant could reach 30,000 U/mL, which meets most of the conditions for the industrial production [ 33 ]. Therefore, TLL is a promising commercial biocatalyst for further modulating.…”

Section: Discussionmentioning

confidence: 99%

Discovery of the Key Mutation Site Influencing the Thermostability of Thermomyces lanuginosus Lipase by Rosetta Design Programs

Zhu

Xia

Wan

et al. 2022

IJMS

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Lipases are remarkable biocatalysts and are broadly applied in many industry fields because of their versatile catalytic capabilities. Considering the harsh biotechnological treatment of industrial processes, the activities of lipase products are required to be maintained under extreme conditions. In our current study, Gibbs free energy calculations were performed to predict potent thermostable Thermomyces lanuginosus lipase (TLL) variants by Rosetta design programs. The calculating results suggest that engineering on R209 may greatly influence TLL thermostability. Accordingly, ten TLL mutants substituted R209 were generated and verified. We demonstrate that three out of ten mutants (R209H, R209M, and R209I) exhibit increased optimum reaction temperatures, melting temperatures, and thermal tolerances. Based on molecular dynamics simulation analysis, we show that the stable hydrogen bonding interaction between H198 and N247 stabilizes the local configuration of the 250-loop in the three R209 mutants, which may further contribute to higher rigidity and improved enzymatic thermostability. Our study provides novel insights into a single residue, R209, and the 250-loop, which were reported for the first time in modulating the thermostability of TLL. Additionally, the resultant R209 variants generated in this study might be promising candidates for future-industrial applications.

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“…The data were analyzed employing one-way and two-way analyses of variance (ANOVA) with the Tukey’s test (honestly significant difference) for multiple comparisons. The values were expressed by averages ± SD, and the level of significance was at p < 0.05, where n = 6 39 .…”

Section: Methodsmentioning

confidence: 99%

A turning point in the bacterial nanocellulose production employing low doses of gamma radiation

Al-Hagar

Abol-Fotouh

2022

Sci Rep

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In the recent years, huge efforts have been conducted to conceive a cost-effective production process of the bacterial nanocellulose (BNC), thanks to its marvelous properties and broadening applications. Herein, we unveiled the impact of gamma irradiation on the BNC yield by a novel bacterial strain Komagataeibacter hansenii KO28 which was exposed to different irradiation doses via a designed scheme, where the productivity and the structural properties of the BNC were inspected. After incubation for 240 h, the highest BNC yield was perceived from the culture treated twice with 0.5 kGy, recording about 475% higher than the control culture. Furthermore, almost 92% of its BNC yield emerged in the first six days. The physicochemical characteristics of the BNCs were investigated adopting scanning electron microscope (SEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and Fourier transform infrared (FTIR). Additionally, the water holding capacity, water release rate, surface area (BET), and mechanical properties were configured for the BNC generated from the control and the irradiated cultures. As a whole, there were no significant variations in the properties of the BNC produced by the irradiated cultures versus the control, proposing the strain irradiation as a valuable, facile, and cheap route to augment the BNC yield.

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Optimization, purification, and biochemical characterization of thermoalkaliphilic lipase from a novel Geobacillus stearothermophilus FMR12 for detergent formulations

Cited by 50 publications

References 36 publications

Optimization and characterization of alkaliphilic lipase from a novel Bacillus cereus NC7401 strain isolated from diesel fuel polluted soil

Optimization and characterization of alkaliphilic lipase from a novel Bacillus cereus NC7401 strain isolated from diesel fuel polluted soil

Discovery of the Key Mutation Site Influencing the Thermostability of Thermomyces lanuginosus Lipase by Rosetta Design Programs

A turning point in the bacterial nanocellulose production employing low doses of gamma radiation

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