Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography

Berhow, Mark A.; Polat, Ümit; Gliński, Jan A.; Gleńsk, Michał; Vaughn, Steven F.; Isbell, Terry A.; Ayala-Diaz, Ivan; Marek, Laura F.; Gardner, C

doi:10.1016/j.indcrop.2012.07.018

Cited by 86 publications

(70 citation statements)

References 25 publications

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“…Glucosinolate analysis is more complicated. The original methodology was to analyze desulfonated forms of the glucosinolates [33,40,47], but if good ion-pairing agents are used, reverse phase chromatographic analysis can be carried out on intact glucosinolates extracted from the meal [33,38,60].…”

Section: Phytochemical Characterization Methodsmentioning

confidence: 99%

“…Current breeding work in the USA, Canada, and Finland, coupled with the analytical methodology discussed in this review, indicate that it is possible to reduce or eliminate some of the taste components of camelina that currently prevent higher percentages in feed blends. The unique character of the camelina glucosinolates, especially since they are similar in structure when converted to isothiocyanate form to the anticancer constituent sulforaphane identified in broccoli, makes camelina meal even more interesting as a functional food and as a source of nutraceuticals [33,38].…”

Section: Camelina Meal Usesmentioning

confidence: 99%

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Evaluating the Phytochemical Potential of Camelina: An Emerging New Crop of Old World Origin

Berhow¹,

Vaughn²,

Moser³

et al. 2014

Phytochemicals – Biosynthesis, Function and Application

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Out on the next frontier of nutritional research will be the complete biochemical and physiological characterization of plant-derived foods that prevent or delay the development of chronic diseases in humans and animals. The chemical composition of many major crop products (seeds, flour, oil, leaves, etc.) have been determined, but the slow process of evaluating each compound alone or in mixtures for the biological function in nutrition and health of the animals that consume them has only just begun. Camelina, or false flax ( Camelina sativa L. Crantz), is an emerging oil seed crop in North America mostly used as a biodiesel fuel. The seeds contain up to 45 % oil, which is rich in polyunsaturated omega-3 and omega-6 fatty acids, as well as fat-soluble antioxidants such as the vitamin E-active tocopherols. Extraction of oil from camelina seeds by mechanical expeller yields a seed meal that consists of approximately 10 % residual oil, 45 % crude protein, 10 % soluble sugars, 13 % fiber, 5 % minerals, and 10 % phytochemical constituents such as glucosinolates, flavonols, lignans, phenolic acids as well as nucleic acids. The seed meal also contains a hydrophilic gum. While the oil fraction has been well characterized and its uses are growing, the seed meal has yet to be fully characterized for its potential use Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Section: Phytochemical Characterization Methodsmentioning

confidence: 99%

Section: Camelina Meal Usesmentioning

confidence: 99%

Evaluating the Phytochemical Potential of Camelina: An Emerging New Crop of Old World Origin

Berhow¹,

Vaughn²,

Moser³

et al. 2014

Phytochemicals – Biosynthesis, Function and Application

Self Cite

View full text Add to dashboard Cite

show abstract

“…GLS content of the samples were also analyzed according to the methodology of Berhow et al [21]. Samples were run on a LTQ-XL Orbitrap MS (a linear ion trap), coupled to a high precision electrostatic ion trap mass spectrometer (MS) (LTQ XL, Thermo Scientific, Waltham, MA, USA) with an Ion Max electrospray ionization (ESI) source, coupled to an ACELLA series HPLC (ACELLA 1250 UHPLCS pump, ACCELA1 HTC cool stack auto-injector, and an ACELLA 80 Hz photodiode array detector) (Thermo Scientific).…”

Section: Gls Determinationmentioning

confidence: 99%

Solid State Fermentation of Carinata (Brassica carinata) Meal Using Various Fungal Strains to Produce a Protein-Rich Product for Feed Application

Simon¹,

Wootton²,

Johnson³

et al. 2017

J Microb Biochem Technol

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“…Individual GLS were confirmed to be present by quadrupole time-of-flight liquid chromatography-mass spectrometry and quantified using reverse phase HPLC (Berhow et al, 2013). For GLS quantitation, a modification of a HPLC method, developed by Betz and Fox (1994), was used.…”

Section: Glucosinolatesmentioning

confidence: 99%

Enhancing the Nutritional Value of Canola (Brassica napus) Meal Using a Submerged Fungal Incubation Process

Croat

Gibbons

Berhow

et al. 2016

JFR

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The aim of this study was to determine the optimal fungal culture to increase the nutritional value of canola meal so it could be used at higher feed inclusion rates, and for a broad range of monogastrics, including fish. Submerged incubation conditions were used to evaluate the performance of seven fungal cultures in hexane extracted (HE) and cold pressed (CP) canola meal. Aureobasidium pullulans (Y-2311-1), Fusarium venenatum and Trichoderma reesei resulted in the greatest improvements in protein levels in HE canola meal, at 21.0, 23.8, and 34.8 %, respectively. These fungi reduced total glucosinolates (GLS) content to 2.7, 7.4, and 4.9 μM.g -1 , respectively, while residual sugar levels ranged from 0.8 to 1.6 % (w/w). In trials with CP canola meal, the same three fungi increased protein levels by 24.6, 35.2, and 37.3 %, and final GLS levels to 6.5, 4.0, and 4.7 μM.g -1 , respectively. Additionally, residual sugar levels were reduced to 0.3-1.0 % (w/w).

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Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography

Cited by 86 publications

References 25 publications

Evaluating the Phytochemical Potential of Camelina: An Emerging New Crop of Old World Origin

Evaluating the Phytochemical Potential of Camelina: An Emerging New Crop of Old World Origin

Solid State Fermentation of Carinata (Brassica carinata) Meal Using Various Fungal Strains to Produce a Protein-Rich Product for Feed Application

Enhancing the Nutritional Value of Canola (Brassica napus) Meal Using a Submerged Fungal Incubation Process

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