Optimized and validated spectrophotometric determination of two antifungal drugs in pharmaceutical formulations using an ion-pair complexation reaction

Abstract

Simple, rapid, cost-effective, sensitive and extractive spectrophotometric methods were developed for the determination of two antifungal drugs (i.e., sertaconazole nitrate (SER) and miconazole nitrate (MCO)) in pure and dosage forms. The methods are based on the formation of ion-pair complexes between the drugs and acid dyes (i.e., bromocresol purple (BCP), bromophenol blue (BPB) and methyl orange (MO)) in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 410, 416 and 427 nm for SER and at 408, 415 and 426 nm for MCO using BCP, BPB and MO, respectively. The analytical parameters and their effects on the reported systems were investigated. Beer's law was obeyed in the 1.0-20 and 1.0-24 g mL −1 ranges for SER and MCO, respectively. The composition of the ion pairs was determined to be 1:1. The molar absorptivity, Sandell sensitivity, limits of detection and limits of quantification were calculated. Other method validation parameters, such as precision, accuracy, robustness, ruggedness and selectivity, were satisfactory. The proposed methods have been successfully applied for the analysis of the studied drugs in their pure and dosage forms. Statistical comparison of the results with the reference methods indicated excellent agreement and no significant difference in accuracy and precision.

Application of TLC-Spectrodensitometric and Chemometric Methods for Determination of Momenta® Cream: A Comparative Study Applied on Ternary Mixture

“…Miconazole Nitrate (MIC) an imidazole antifungal agent that is used topically and by intravenous infusion, miconazole nitrate is the nitrate salt form of miconazole, an antifungal synthetic derivative of imidazole and used in the treatment of candidal skin infections. A review of the literature showed that methods reported for the determination of (MIC) alone or in combinations were spectrophotometry [21][22][23][24][25][26][27][28], HPLC [29][30][31][32][33][34][35], HPTLC [31,[36][37][38], gas chromatography [39,40], and electrochemical methods [41]. Gentamicin (GM) is a mixture of isomeric aminoglycoside antibiotics (gentamicin C 1 , gentamicin C 1A , and gentamicin C 2 ) produced by Micromonospora purpurea or M. echinospora.…”

Spectrophotometric Assay of Diethylcarbamazine Citrate in Pharmaceuticals and Human Urine via Ion-Pair Reaction Using Methyl Orange Dye

“…Few methods based on color reactions like condensation [20] and charge-transfer complexation [21] are found in the literature for the determination of DEC in bulk drug and dosage forms. Extractivespectrophotometry is widely used in pharmaceutical analysis [22][23][24][25][26][27][28][29][30] because of its sensitivity and selectivity; the technique also finds use in the assay of DEC in pharmaceuticals. The charge-transfer complex formed by the interaction between the chloranilic acid and DEC [31] was adopted for the assay of the drug in pure powder and in tablets.…”