Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

Junglee, Sanders; Urban, Laurent; Sallanon, Huguette; Lopez-Lauri, Félicie

doi:10.4236/ajac.2014.511081

Cited by 336 publications

(155 citation statements)

References 11 publications

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“…Two weeks old T 2 homozygous transgenic (overexpression and downregulation) with WT plants were treated with 150 mM NaCl for different time durations (1, 6, 12, and 24 hr), and untreated plants were used as control. Various assays such as hydrogen peroxide (Junglee, Urban, Sallanon, & Lopez-Lauri, 2014), lipid peroxidation (Dhindsa, Plumb-Dhindsa, & Thorpe, 1981), ascorbate peroxidase (Nakano & Asada, 1981), catalase (Garg, Jaiswal, Misra, Tripathi, & Prasad, 2012), proline estimation (Bates, Waldren, & Teare, 1973) and cell membrane stability (Farooq & Azam, 2006) were performed with treated (WT, control, and transgenic) plants.…”

Section: Biochemical Analysismentioning

confidence: 99%

Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis

Passricha

Saifi

Kharb

et al. 2019

Biotech & Bioengineering

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Sensing stress and activating the downstream signaling pathways is the imperative step for stress response. Lectin receptor‐like kinase (LecRLK) is an important family that plays a key role in sensing stress conditions through lectin receptor and activates downstream signaling by kinase domain. We identified the role of OsLecRLK gene for salinity stress tolerance and hypothesized its role in Na+ extrusion from cell. OsLecRLK overexpression and downregulation (through artificial miRNA) transgenic lines were developed and its comparison with wild‐type (WT) plants were performed overexpression transgenic lines showed better performance, whereas downregulation showed poor performance than WT. Lower accumulation of reactive oxygen species (ROS), malondialdehyde and toxic ion, and a higher level of proline, RWC, ROS scavengers in overexpression lines lead us to the above conclusion. Based on the relative expression of stress‐responsive genes, ionic content and interactome protein, working model highlights the role of OsLecRLK in the extrusion of Na+ ion from the cell. This extrusion is facilitated by a higher expression of salt overly sensitive 1 (Na+/K+ channel) in overexpression transgenic line. Altered expression of stress‐responsive genes and changed biochemical and physiological properties of cell suggests an extensive reprogramming of the stress‐responsive metabolic pathways by OsLecRLK under stress condition, which could be responsible for the salt tolerance capability.

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Section: Biochemical Analysismentioning

confidence: 99%

Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis

Passricha

Saifi

Kharb

et al. 2019

Biotech & Bioengineering

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“…The hydrogen peroxide (H 2 O 2 ) concentration in all of the tested variants was determined according to Velikova et al (2000) (but also see Junglee et al 2014). Briefly, coleoptile segment samples of 0.5 g were homogenised in 1.5 ml of 0.1% (w/v) tri-chloroacetic acid (TCA).…”

Section: Hydrogen Peroxide Detectionmentioning

confidence: 99%

A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells

2017

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“…An aliquot of 0.5 mL of the supernatant was added to 0.5 mL of KH 2 PO 4 buffer (10 mmol L −1 , pH 7) and 1 mL of fresh 1 mol L −1 KI. The mixture was incubated at 25 °C in the dark for 1 h. The absorbance was read at 350 nm . The quantification of hydrogen peroxide was based on a calibration curve obtained with H 2 O 2 standard solutions prepared in 0.1% trichloroacetic acid and expressed as µmol of H 2 O 2 g −1 of dry weight.…”

Section: Methodsmentioning

confidence: 99%

Influence of Acacia dealbata Link bark extracts on the growth of Allium cepa L. plants under high salinity conditions

et al. 2019

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BACKGROUND Acacia dealbata Link is an invasive plant worldwide. Finding potential uses for its waste that contribute to controling its spread and result in benefits for agriculture has recently become a new topic of research. This study aimed to evaluate the potential biostimulant effect of bark extract derived from the management of A. dealbata (0, 450 or 900 ppm) on onion plants growing under stressful conditions, such as in soils treated with saline solutions (0, 60 or 120 mmol L−1 NaCl) and with reduced irrigation (100%, 50% or 25%). RESULTS A. dealbata Link bark extract significantly increased height as well as leaf, root and total biomass of plants in soils irrigated with NaCl solution (120 mmol L−1). These plants also had a higher content of Cl in roots, but a lower content of P in leaves and of K in bulbs. The 450 ppm bark treatment additionally increased the protein content in leaves and decreased the Na and Mg content in bulbs and bulbs and roots, respectively. The bark extract also increased the sugar content in plants under saline conditions. However, the effect of bark extract was negligible on plants that grew under drought stress. CONCLUSION Results revealed that the bark extract might attenuate stress effects in plants growing at high salinity levels, probably by increasing their sugar and protein content and via the accumulation of ions in the roots. Although additional experiments are required, we suggest that the bark extract of A. dealbata has potential applications in agriculture concerned with biostimulant formulations. © 2019 Society of Chemical Industry

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Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

Cited by 336 publications

References 11 publications

Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis

Rice lectin receptor‐like kinase provides salinity tolerance by ion homeostasis

A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells

Influence of Acacia dealbata Link bark extracts on the growth of Allium cepa L. plants under high salinity conditions

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