Optimized astaxanthin production in Chlorella zofingiensis under dark condition by response surface methodology

Chen, Tao; Wang, Yan

doi:10.1007/s10068-013-0221-7

Cited by 8 publications

(5 citation statements)

References 30 publications

(33 reference statements)

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“…Statistical analysis has been applied on algal biomass and lipid production a lot, because it is an efficient way to optimize complex and various culture conditions including nutrient, temperature, pH and so on 22–24 . However, statistical optimizations were limited to phototrophic cultivation of microalgae, and the application for heterotrophic cultivation has been seldom reported.…”

Section: Introductionmentioning

confidence: 99%

Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

Kim

Park

Lee

et al. 2019

Sci Rep

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The heterotrophic cultivation of microalgae has a number of notable advantages, which include allowing high culture density levels as well as enabling the production of biomass in consistent and predictable quantities. In this study, the full potential of Chlorella sp. HS2 is explored through optimization of the parameters for its heterotrophic cultivation. First, carbon and nitrogen sources were screened in PhotobioBox. Initial screening using the Plackett-Burman design (PBD) was then adopted and the concentrations of the major nutrients (glucose, sodium nitrate, and dipotassium phosphate) were optimized via response surface methodology (RSM) with a central composite design (CCD). Upon validation of the model via flask-scale cultivation, the optimized BG11 medium was found to result in a three-fold improvement in biomass amounts, from 5.85 to 18.13 g/L, in comparison to a non-optimized BG11 medium containing 72 g/L glucose. Scaling up the cultivation to a 5-L fermenter resulted in a greatly improved biomass concentration of 35.3 g/L owing to more efficient oxygenation of the culture. In addition, phosphorus feeding fermentation was employed in an effort to address early depletion of phosphate, and a maximum biomass concentration of 42.95 g/L was achieved, with biomass productivity of 5.37 g/L/D.

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Section: Introductionmentioning

confidence: 99%

Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

Kim

Park

Lee

et al. 2019

Sci Rep

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“…High glucose and low nitrate are optimal for astaxanthin production in batch cultures [ 89 , 91 ], although a higher astaxanthin yield of 11.14 mg/L was obtained from fed-batch culture with a combined glucose–nitrate mixture [ 91 ]. Moreover, a two-stage heterotrophic strategy where the media changed from 46.7, 1.13, and 0.125 g/L to 35.2, 0.281, and 0.023 g/L glucose, nitrate, and MgSO 4 ·7H 2 O, respectively, revealed that the highest astaxanthin yield (0.96 mg/g or 15.1 mg/L) was 74% higher than that in standard media [ 29 ]. After fermentation with 20 g/L glucose, the highest astaxanthin content reported was 73.3 mg/L or 0.07% DW at a cell density of 98.4 g/L which was considered to be comparable and even higher than quantities achieved from H. pluvialis [ 101 ].…”

Section: Effect Of Culture Conditions Stress and Trophic Mode On Cell...mentioning

confidence: 99%

“…C. zofingiensis is one of the most commonly mentioned microalgal species for implementation into a biorefinery [ 5 , 9 , 16 , 17 , 29 ]. However, despite being used as an example, limited experimental research has taken place to obtain multiple products.…”

Section: Introductionmentioning

confidence: 99%

Progress towards a targeted biorefinery of Chromochloris zofingiensis: a review

Wood

Ross

Jubeau³

et al. 2022

Biomass Conv. Bioref.

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Biorefinery approaches offer the potential to improve the economics of the microalgae industry by producing multiple products from a single source of biomass. Chromochloris zofingiensis shows great promise for biorefinery due to high biomass productivity and a diverse range of products including secondary carotenoids, predominantly astaxanthin; lipids such as TAGs; carbohydrates including starch; and proteins and essential amino acids. Whilst this species has been demonstrated to accumulate multiple products, the development of an integrated downstream process to obtain these is lacking. The objective of this review paper is to assess the research that has taken place and to identify the steps that must be taken to establish a biorefinery approach for C. zofingiensis. In particular, the reasons why C. zofingiensis is a promising species to target for biorefinery are discussed in terms of cellular structure, potential products, and means to accumulate desirable components via the alteration of culture conditions. Future advances and the challenges that lie ahead for successful biorefinery of this species are also reviewed along with potential solutions to address them.

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“…Chlorella zofingiensis optimized the Basal medium using a Plackett-Burman design to peruse seven different factors in which glucose, sodium nitrate and magnesium sulfate heptahydrate were identified to maximize both cell growth and astaxanthin production (Chen &Wang, 2013).…”

Section: Determination Of Carotenoid Concentration and Statistical Analysismentioning

confidence: 99%

Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Şener¹,

Demirel²,

İmamoğlu³

et al. 2021

ase

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Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliella salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m 2 s, and in the nutrient component of 8.82 mM NaNO 3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β-carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus.

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Optimized astaxanthin production in Chlorella zofingiensis under dark condition by response surface methodology

Cited by 8 publications

References 30 publications

Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

Progress towards a targeted biorefinery of Chromochloris zofingiensis: a review

Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

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