Optimized liquid chromatography–mass spectrometry approach for the isolation of minor stress biomarkers in plant extracts and their identification by capillary nuclear magnetic resonance

Spatial and Temporal Dynamics of Jasmonate Synthesis and Accumulation in Arabidopsis in Response to Wounding

“…For experiments presented in Figs. 2, 3, 4B, and 5, the Glauser et al (21) sample preparation protocol was used. The rosette leaves on one half of the plant were crushed across the leaf apices with forceps, wounding ϳ30% of the leaf area.…”

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

“…Additionally the SPE step allows the use of normal nondeuterated HPLC eluents and the recording of NMR spectra in 100% deuterated solvents. Combining LC/ SPE with off-line capillary NMR is not trivial but ideally leads to a much improved mass sensitivity allowing the recording of the 2D spectra of as little as 25 lg and 1D spectra of sub lg quantities (Lambert et al 2007;Glauser et al 2008). Interestingly Lin et al (2008) do not use SPE in their LC/UV/MS/ NMR system but collect the fractions in 96 well plates.…”

Proteomic Analyses of Cells Isolated by Laser Microdissection

“…So far, studies in this field have www.intechopen.com mainly been performed on whole plants, organs, such as fruits (Moco et al, 2006;Fraser et al, 2007), leaves (Kant et al, 2004;Glauser et al, 2008), tubers (Roessner et al, 2001;Sturm et al, 2007), flowers (Kazuma et al, 2003;Wang et al, 2004), and roots (Opitz et al, 2002;Hagel et al, 2008;). However, some studies have also been performed on specific tissues (Moco et al, 2007;Fait et al, 2008) and even on specific cells Schneider & Hölscher, 2007).…”