Optimized micellar electrokinetic chromatographic separation of benzodiazepines

Renou-Gonnord, Marie-France; David, K.

doi:10.1016/0021-9673(95)00810-1

Cited by 31 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The migration velocity and hence the migration times of BZDs depends on the partitioning of the solute between the CD and the micelle (Figure 3). 8,15,16 This technique is limited to solutes, which can fit into the CD cavity. The ratio of the solute incorporated into the micelle depends on its hydrophobicity but the inclusion complex formation of the solute with CD depends on the concordance of the solute molecular size with the CD cavity diameter.…”

Section: 10mentioning

confidence: 99%

“…The ratio of the solute incorporated into the micelle depends on its hydrophobicity but the inclusion complex formation of the solute with CD depends on the concordance of the solute molecular size with the CD cavity diameter. 13,15 Using a molecular modulation program, Chem3D (CambridgeSoft), we followed the molecular complexation of BZDs with CDs. Having a rigid structure with a benzene ring condesated to a diazepinic one the complexation process for BZDs is difficult and far from perfect.…”

Section: 10mentioning

confidence: 99%

“…Among various CDs, γ-CD in general is considered to be most effective in MEKC separations, 14,15 but in this work we chose β-CD arguably the most frequently used CD for the complexation of chemical compounds of pharmaceutical interest.…”

Section: 10mentioning

confidence: 99%

“…High concentration of CDs may lead to an increase of the solubilization in the CD ca- vity, and migration of the solutes with the EOF. 15 In this work, the CD concentration was set at 15 mmol dm . The solubility of β-CD in water is relatively low.…”

Section: 10mentioning

confidence: 99%

See 3 more Smart Citations

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Hancu¹,

Gyéresi²

2011

Croat. Chem. Acta

View full text Add to dashboard Cite

Abstract. The applicability of micellar electrokinetic capillary chromatography (MEKC) for the separation of benzodiazepine (BZD) derivates with different structural characteristics has been studied. Efforts were first focused on the optimization of the analytical conditions, on the effects of buffer concentration, surfactant concentration and buffer pH on the separation. To manipulate selectivity of the separation β-cyclodextrin (CD) was added to the buffer solution, and urea was used to improve the solubility of the CD in water. The CD-modified MEKC separation of the eight BZDs was achieved within 15 minutes using 25 mmol dm −3 natrium tetraborate-50 mmol dm −3 sodium dodecyl sulphate-15 mmol dm −3 β-CD-2 mol dmurea (pH 9.3) as the running buffer. The proposed separation method was evaluated on the basis of precision, linearity and limit of detection.

show abstract

Section: 10mentioning

confidence: 99%

Section: 10mentioning

confidence: 99%

Section: 10mentioning

confidence: 99%

Section: 10mentioning

confidence: 99%

See 2 more Smart Citations

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Hancu¹,

Gyéresi²

2011

Croat. Chem. Acta

View full text Add to dashboard Cite

show abstract

“…The weak solvent strength of purely micellar eluents in the reversed-phase liquid chromatography can be increased by the addition of an organic modifier [2,3], which can also improve the efficiency of the chromatographic peaks [4]. Micellar mobile phase shows several advantages compared to the conventional hydro-organic eluents such as low cost, low toxicity, the possibility of simultaneous separation of ionic and nonionic compounds without needing a gradient elution, direct injection of biological fluids due to the solubility of proteins in the micelles, low volatility, detection sensitivity enhancement, unique separation selectivity [5][6][7][8], application in quantitative structureactivity relationship studies [9].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the separation of coumarins in mixed micellar liquid chromatography using Derringer's desirability function

Ebrahimi

Hadjmohammadi

2007

Journal of Chemometrics

View full text Add to dashboard Cite

The multi-criteria decision making (MCDM) method was applied for the optimization of the separation of eight coumarin compounds in mixed micellar liquid chromatography (MLC). In order to achieve this goal the usefulness of Derringer's desirability function for the evaluation of the two different chromatographic performance goals (resolution and analysis time) was tested. The effect of six experimental parameters on a chromatographic response function (CRF) expressed as a product of two sigmoidal desirability functions was investigated. The sigmoidal functions were used to transform the optimization criteria, resolution and analysis time, into the desirability values. The factors studied were the concentration of sodium dodecyl sulfate (SDS) and polyxyethylene (23) dodecanol (Brij-35), alkyl chain length of the alcohol used as the organic modifier, organic modifier concentration, mobile phase pH and temperature. For the development of the method and the optimization of the chromatographic conditions, the experiments were performed according to a face-centered cube response surface experimental design. The qualities of the obtained chromatograms were calculated using CRF and model was built by the use of MLR to correlate the obtained CRFs to the variables and their interactions. The MLR model developed showed good descriptive and predictive ability (R 2 ¼ 0.998, F ¼ 626.056, SE ¼ 0.009, R 2 CV ¼ 0.985) and was used, by a grid search algorithm, to optimize the chromatographic conditions for the separation of the mixture. The efficiency of prediction of polynomial model was confirmed by performing the experiment under the optimal conditions. The results of the study indicated that a CRF composed of sigmoid transformations can also be successfully used for the evaluation of the chromatograms.

show abstract

Identiﬁcation and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography

Zheng

Wang

Chen

et al. 2003

Biomedical Chromatography

View full text Add to dashboard Cite

The simultaneous determination of emodin, aloe-emodin and rhein, in Semen Cassiae, Qinghai Wild Dahuang tea, Ningxia Juemingzi tea, Lanzhou Juemingzi tea and Surong Juemingzi tea, has been investigated by micellar electrokinetic capillary chromatography for the fi rst time. With an electrolyte containing 15 mm borax, 30 mm SDS, 10% (v/v) ethanol, at pH 9.60 and 20 kV applied voltage, the three analytes were completely separated within 12 min. The effects of the concentration of borax, electrolyte pH, the concentrations of SDS and organic modifier and the applied voltage on electropheoretic behavior and separation were studied. The linear calibration range was 4-120 micro g/mL (r = 0.9921) for emodin, 10-200 micro g/mL (r = 0.9970) for aloe-emodin and 2-100 micro g/mL (r = 0.9971) for rhein, respectively. Under the optimum conditions, the relative standard deviation (RSD) values (n = 6) of the migration time and the peak area of each peak was 0.59-0.80% and 1.30-3.22%, respectively. The contents of the analytes in Rheum, Qinghai Wild Dahuang tea, Semen Cassiae and three other kinds of teas were easily determined with recoveries ranging from 95.3 to 104.4%.

show abstract

Optimized micellar electrokinetic chromatographic separation of benzodiazepines

Cited by 31 publications

References 17 publications

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Optimization of the separation of coumarins in mixed micellar liquid chromatography using Derringer's desirability function

Identiﬁcation and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography

Contact Info

Product

Resources

About