2024
DOI: 10.1002/rcm.9764
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Optimized quantification of fatty acids in complex media using advanced analytical techniques

Eunji Jeon,
Moonhee Park,
Abhik Mojumdar
et al.

Abstract: RationaleVarious medium formulations contain essential fatty acids at concentrations ranging from 10 to 100 mg/L. Accurate and precise lipid measurement in media is crucial for monitoring media quality and conducting studies on lipids in the context of cell culture. This study employed two‐dimensional gas chromatography (GC × GC) analyses to offer enhanced resolution, sensitivity, and separation performance compared to GC.MethodsQuantification of fatty acid methyl esters (FAMEs) in a medium was conducted using… Show more

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