Optimized Rhodobacter sphaeroides for the Production of Antioxidants and the Pigments with Antioxidant Activity

Lee, Subin; Yu, Jaeyoung; Kim, Yang‐Hoon; Min, Jiho

doi:10.1007/s12033-022-00547-4

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…3 ). Particularly, Rbs Anaero exhibited higher antioxidant activity at all treatment concentrations than FBS and Rbs Aero , indicating that the anaerobically grown R. sphaeroides extract contained more antioxidant compounds than Rbs Aero ( 13 , 14 ). However, it was observed that both culture conditions provided a significant increase in antioxidant activity in HS27 cells.…”

Section: Discussionmentioning

confidence: 91%

“…Following a 48-hour seed culture, the main culture was initiated and grown at 30°C and 180 rpm until it reached the stationary phase. Upon cell growth, pellets were obtained by centrifugation, dissolved in distilled water, and subjected to sonication for 25 minutes (on:off = 30 seconds:59 seconds) using a 20% pulse ( 13 ). Subsequently, cell debris was removed by centrifugation at 13,000 rpm for 10 minutes, and intracellular substances were extracted using a 0.2-µm syringe filter ( 36 ).…”

Section: Methodsmentioning

confidence: 99%

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The potential of Rhodobacter sphaeroides extract as an alternative supplement for cell culture systems

Lee,

Kim,

Min

2024

Microbiol Spectr

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The choice of supplements for cell culture is crucial in biomedical research, but the widely used fetal bovine serum (FBS) has limitations in terms of variability, ethics, and environmental risks. This study explores the potential of an extract from Rhodobacter sphaeroides , a probiotic bacterium, as an alternative supplement. The findings reveal that the R. sphaeroides extract surpasses FBS in enhancing cell growth, viability, and functionality. It also mitigates oxidative stress and stimulates lysosomal activity, critical for cellular health. The extract’s abundance of glycine and arginine, amino acids with known growth-promoting effects, further highlights its potential. By providing a viable substitute for FBS, the R. sphaeroides extract addresses the need for consistent, ethical, and environmentally friendly cell culture supplements. This research paves the way for sustainable and reliable cell culture systems, revolutionizing biomedical research and applications in drug development and regenerative medicine.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

The potential of Rhodobacter sphaeroides extract as an alternative supplement for cell culture systems

Lee,

Kim,

Min

2024

Microbiol Spectr

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“…One of the microorganisms that can synthesize CoQ 10 efficiently and has been used in industrial production is Rhodobacter sphaeroides [ 13 , 14 ]. It is a gram-negative purple non sulfur bacterium (PNSB) that can grow aerobically, anaerobically, photoautotrophically, and heterotrophically [ 15 – 17 ]. It has many other functions, such as biological hydrogen production, synthesis of carotenoids, fixation of CO 2 and N 2 , remediation of heavy metal pollution and power generation [ 18 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators

Xiao,

Zheng,

Zhou

et al. 2023

Microb Cell Fact

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Coenzyme Q10 (CoQ10) is crucial for human beings, especially in the fields of biology and medicine. The aim of this experiment was to investigate the conditions for increasing CoQ10 production. At present, microbial fermentation is the main production method of CoQ10, and the production process of microbial CoQ10 metabolism control fermentation is very critical. Metabolic flux is one of the most important determinants of cell physiology in metabolic engineering. Metabolic flux analysis (MFA) is used to estimate the intracellular flux in metabolic networks. In this experiment, Rhodobacter sphaeroides was used as the research object to analyze the effects of aqueous ammonia (NH3·H2O) and calcium carbonate (CaCO3) on the metabolic flux of CoQ10. When CaCO3 was used to adjust the pH, the yield of CoQ10 was 274.43 mg·L−1 (8.71 mg·g−1 DCW), which was higher than that of NH3·H2O adjustment. The results indicated that when CaCO3 was used to adjust pH, more glucose-6-phosphate (G6P) entered the pentose phosphate (HMP) pathway and produced more NADPH, which enhanced the synthesis of CoQ10. At the chorismic acid node, more metabolic fluxes were involved in the synthesis of p-hydroxybenzoic acid (pHBA; the synthetic precursor of CoQ10), enhancing the anabolic flow of CoQ10. In addition, Ca2+ produced by the reaction of CaCO3 with organic acids promotes the synthesis of CoQ10. In summary, the use of CaCO3 adjustment is more favorable for the synthesis of CoQ10 by R. sphaeroides than NH3·H2O adjustment. The migration of metabolic flux caused by the perturbation of culture conditions was analyzed to compare the changes in the distribution of intracellular metabolic fluxes for the synthesis of CoQ10. Thus, the main nodes of the metabolic network were identified as G6P and chorismic acid. This provides a theoretical basis for the modification of genes related to the CoQ10 synthesis pathway.

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Bioconversion of L-Tyrosine into p-Coumaric Acid by Tyrosine Ammonia-Lyase Heterologue of Rhodobacter sphaeroides Produced in Pseudomonas putida KT2440

Calderón,

Gentina,

Evrard

et al. 2024

CIMB

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p-Coumaric acid (p-CA) is a valuable compound with applications in food additives, cosmetics, and pharmaceuticals. However, traditional production methods are often inefficient and unsustainable. This study focuses on enhancing p-CA production efficiency through the heterologous expression of tyrosine ammonia-lyase (TAL) from Rhodobacter sphaeroides in Pseudomonas putida KT2440. TAL catalyzes the conversion of L-tyrosine into p-CA and ammonia. We engineered P. putida KT2440 to express TAL in a fed-batch fermentation system. Our results demonstrate the following: (i) successful integration of the TAL gene into P. putida KT2440 and (ii) efficient bioconversion of L-tyrosine into p-CA (1381 mg/L) by implementing a pH shift from 7.0 to 8.5 during fed-batch fermentation. This approach highlights the viability of P. putida KT2440 as a host for TAL expression and the successful coupling of fermentation with the pH-shift-mediated bioconversion of L-tyrosine. Our findings underscore the potential of genetically modified P. putida for sustainable p-CA production and encourage further research to optimize bioconversion steps and fermentation conditions.

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Optimized Rhodobacter sphaeroides for the Production of Antioxidants and the Pigments with Antioxidant Activity

Cited by 3 publications

References 12 publications

The potential of Rhodobacter sphaeroides extract as an alternative supplement for cell culture systems

The potential of Rhodobacter sphaeroides extract as an alternative supplement for cell culture systems

Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators

Bioconversion of L-Tyrosine into p-Coumaric Acid by Tyrosine Ammonia-Lyase Heterologue of Rhodobacter sphaeroides Produced in Pseudomonas putida KT2440

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