Optimizing culture conditions for high frequency somatic embryogenesis and plantlet conversion in Daucus carota L

Sundararajan, Sathish; Nayeem, Safia; Shanmugam, Sivakumar; Venkatesh, R.; Ramalingam, Sathishkumar

doi:10.2478/s11756-019-00223-0

Cited by 2 publications

(5 citation statements)

References 35 publications

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“…Stem explants showed the best induction of embryogenic and friable callus. It has been reported that stem explants are an excellent source for the production of somatic embryos from callus compared with all other explants [2,26]. Our results corroborate that stem explants present efficient callus formation and viable suspension cell cultures.…”

Section: Discussionsupporting

confidence: 88%

“…Our results corroborate that stem explants present efficient callus formation and viable suspension cell cultures. It has also been reported that high concentrations of 2,4-D (above 1 mg/L) added to the culture medium developed different types of individual cells in the suspension cells of Daucus carota [2,27]. In this study, suspension cell cultures were established with embryogenic and friable calluses derived from stem explants.…”

Section: Discussionmentioning

confidence: 87%

“…The cell suspensions showed a rapid proliferation because of the use of friable callus during the first 35 days of culture. The effective induction and proliferation of callus and cell suspensions was due to the use of 2,4-D [2,28].…”

Section: Discussionmentioning

confidence: 99%

“…The seeds were disinfected in 70% ethanol for 5 min, followed by immersion in 5% (v/v) sodium hypochlorite for 15 min. Afterward, the seeds were washed three times in sterile distilled water [2]. The seeds were placed in tissue culture flasks with 1 /2 MS medium [49] supplemented with 3% sucrose and 2.5% Phytagel ® .…”

Section: Plant Materialsmentioning

confidence: 99%

“…The carrot (Daucus carota L.) is a member of the Umbelliferae family, and it is a biennial plant. In addition to being consumed as food, different parts of the carrot can be used for medicinal purposes [2], such as hepatoprotective [3], antisteroidogenic [4], antinociceptive and anti-inflammatory functions [5]. The seeds are used for the treatment of swelling and tumors, and the roots are used as a poultice in mammary and uterine carcinoma as well as for skin cancer [1].…”

Section: Introductionmentioning

confidence: 99%

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Totipotency of Daucus carota L. Somatic Cells Microencapsulated Using Spray Drying Technology

Santiz-Gómez

Abud‐Archila

Sánchez-Roque

et al. 2021

Plants

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The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this work was to evaluate the effect of spray drying on the viability and totipotency of somatic carrot cells. Leaf, root and stem explants were evaluated to induce callus with 2 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). Calli obtained from the stem were cultivated in a liquid medium with 1 mg/L of 2,4-D. Cell suspensions were spray dried with maltodextrin-gum Arabic and maltodextrin-xanthan gum mixtures, two outlet air temperatures (50 and 60 °C) and 120 °C inlet air temperature. Results showed that carrot cells were viable after spray drying, and this viability remained for six months at 8 °C. The totipotency of the microencapsulated cells was proven. Cells that were not spray dried regenerated 24.6 plantlets, while the spray dried cells regenerated 19 plantlets for each gram of rehydrated powder. Thus, spray drying allowed researchers to obtain viable and totipotent cells. This work is the first manuscript that reported the spray drying of plant somatic cells.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%