Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue

Soni, Anjali; Dubey, A. K.; Gupta, Ankit; Sharma, Radha Mohan; Awasthi, O. P.; Bharadwaj, Chellapilla; Sharma, Nimisha

doi:10.1111/pbr.12690

Cited by 5 publications

(10 citation statements)

References 25 publications

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“…Diluted samples were stored at 4 °C for immediate use, while the original concentrates were kept for long-term storage at −20 °C. SSR analysis A set of 37 SSR primers (Soni et al 2019;Tan et al 2007;Ruiz et al 2000) with lengths of 20 or more bp were selected based on the polymorphism information content (PIC) observed in previous studies.…”

Section: Induction Of Embryo Germinationmentioning

confidence: 99%

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Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Dubey

Gupta

Sharma

et al. 2020

Journal of Horticultural Research

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Nucellar embryony is the major obstacle in getting hybrid seedlings in interspecific crosses in citrus. Hence, the present study was conducted to standardize embryo age, culture media for maximizing germination, and subsequent identification of simple sequence repeat (SSR) markers to differentiate the hybrids. A factorial experiment was conducted with three embryo ages – 80–90, 110–120, and 130–140 days after pollination (DAP). The germination of the rescued immature embryos of acid lime × lemon crosses was initiated on three different growing media. The fruits harvested at 130–140 and 80–90 DAP had a higher number of functional seeds (90%). Older embryos (130–140 DAP) and middle-aged embryos (110–120 DAP) germinated in 100% on Murashige and Skoog (MS) medium supplemented with 500 mg·l−1 malt extract, 1.5 mg·l−1 gibberellic acid (GA3), and 0.02 mg·l−1 naphthalene acetic acid (NAA). MS medium supplemented with 500 mg·l−1 malt extract proved better for the germination of embryos taken at 80–90 DAP. Plantlet survival was the highest in younger embryos (80–90 DAP) cultured on MS basal medium (84.21%) and the lowest in older embryos cultured on MS medium supplemented with 500 mg·l−1 malt extract plus 1.5 mg·l−1 GA3 and 0.02 mg·l−1 NAA. The seedlings obtained from the culture 80–90 DAP had the highest root length (4.9 mm) and shoot length (5.3 mm) at 60 days after inoculation (DAI) on the above medium. SSR marker analysis revealed that CCSM-4 and CAC-33 markers expressed polymorphism between female and male parents, proving their ability to identify the hybrids of ‘Kagzi’ acid lime × ‘Konkan Seedless’ lemon.

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Section: Induction Of Embryo Germinationmentioning

confidence: 99%

“…Previously, it was advocated that the sucrose concentration and medium strength impacted the growth and development of the aerial parts, as well as the plantlet root system in the culture of immature Citrus spp. embryos (Soni et al 2019;Pasqual et al 2003;Pérez-Tornero & Porras 2008).…”

Section: Growth Of Plantletsmentioning

confidence: 99%

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Dubey

Gupta

Sharma

et al. 2020

Journal of Horticultural Research

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“…Various studies reported that the addition of 0.01 mg l -1 GA3 (Riberio et al, 2000;Chagas et al, 2005); 0.1 mg l -1 GA3 (Pasqual et al, 1990;Jumin and Nito, 1996;Singh et al, 2020); 1.0 mg l -1 GA3 (Ollitrault et al, 2007;Zhang et al, 2013;Kurt and Ülger, 2014); 1.5 mg l -1 GA3 (Perez- Tornero and Porras, 2008;Soni et al, 2019) and 2.0 mg l -1 GA3 (Gmitter et al, 1990;Turgutoğlu et al, 2015) in growing media for embryos developing of citrus is to be appropriated. Rangan et al (1969) studied nuceller embryos developing in common sour orange.…”

Section: Introductionmentioning

confidence: 99%

“…They indicated that nuceller embryos developing were not seen in growing seeds in 120 days after anthesis. It was determined depending on the examined species and varieties of citrus that 50 days (Wang et al, 1999); 80 days (Tan et al, 2007); 85 days (Xie et al, 2019); 95 days (Singh et al, 2020); 100 days (Tusa et al, 1996;Deng et al, 1996), 105 days (Scarano et al, 2005;Ferrante et al, 2010), 118 days (Chagas et al, 2005); 120 days (Perez-Tornero et al, 2011;Kurt and Ülger, 2019); 130-140 days (Soni et al, 2019) and 135-150 days (Perez-Tornero and Porras, 2008) after pollination was found to be suitable for embryo rescue.…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Embryo Development Stages and GA3 Doses on Germination in Clementine Mandarin × Carrizo Citrange Immature Embryos

Kurt¹,

Turgutoğlu²,

Demir³

2022

Horticultural Studies

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Citrus rootstock breeding is important to improve their resistance to diseases, pests and adverse environmental conditions. The majority of citrus species have nuceller embryony. Although Clementine mandarin is monoembryonic, in some cases (intercrossing etc.) abortive seed formation can be seen frequently. In this study, Clementine mandarin × Carrizo citrange were crossed. In the study, interspecies hybridization (Citrus × Poncirus) was used. Embryo rescue technique was used to prevent the loss of hybrid individuals due to abortive seed formation. The seeds were gathered from the crossed plants 80, 100, and 120 days after pollination. The immature embryos were removed from the seeds and these embryos were transferred to control, 0.5, and 1.0 mg l-1 GA3 containing medium to germination. The highest germination rate with 100% was observed from 1.0 mg l-1 GA3 containing media harvested 120 days after pollination. Additionally, the highest rate of trifoliate seedlings was obtained from embryos gathered after 120 days of the pollination.

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“…The SSR markers are important for rapid assessment of hybrid, more polymorphic, co-dominant and easily reproducible. Recently, Soni et al (2019) have reported three SSR markers (TAA45, CAC15 and CAC39) to be polymorphic between female and male parents of sour orange and Sacaton citrumelo, showing ability to identify their hybrids. Hence, available user-friendly molecular markers need to be selected for early identification of hybrids of Rangpur lime × sour orange crosses.…”

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confidence: 99%

Embryo rescue and hybridity establishment in hybrid progenies derived from polyemebryonic Citrus species

et al. 2021

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Due to highly polyemebryonic nature of Rangpur lime and sour orange, development of hybrid is difficult through conventional breeding. Therefore, the present study was carried out at the Division of Fruits & Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi during 2017-19 to improve hybrid seedling recovery among the crosses made between these species. Germination was induced in rescued immature embryos ages (90-100, 110- 120, 130-140, 150-160 and 170-180 days after pollination) on two culture media (MS medium supplemented with 500 mg/l malt extract +1.5 mg/l GA3 + 0.02 mg/l NAA (M1) and DKW medium added with 1.5 mg/l GA3 + 0.02 mg/l NAA (M2). Fruits harvested at younger stage (90-120 DAP) had higher number of functional seeds. Older embryos (>150 days old) germinated earlier on either medium. Embryos rescued at early stage (90-100 DAP), middle age embryos (130-140 DAP) and oldest embryos (170-180 DAP) germinated efficiently (88.85-100%) on M2 medium. The seedling obtained from 170-180 days old embryos grown on M2 medium produced longest primary root and tallest plantlets (7.52 and 4.48 cm) at 60 days after inoculation (DAI). SSR marker analysis revealed that GT03 loci exhibited the polymorphism between male and female parents, proving their ability to detect the hybrids of Rangpur lime (C. limonia) × sour orange (C. aurantium).

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Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue

Cited by 5 publications

References 25 publications

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Effects of Different Embryo Development Stages and GA3 Doses on Germination in Clementine Mandarin × Carrizo Citrange Immature Embryos

Embryo rescue and hybridity establishment in hybrid progenies derived from polyemebryonic Citrus species

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