Optimizing lipases and related enzymes for efficient application

Bornscheuer, Uwe T.; Bessler, Cornelius; Ramisetti, Srinivas; Krishna, S. Hari

doi:10.1016/s0167-7799(02)02046-2

Cited by 234 publications

(132 citation statements)

References 58 publications

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“…Lipase activity is usually influenced by the pH value of media. In common, lipases from bacteria are active at a wide range of pH value and have neutral or base optimum pH [6,18]. Based on the result exhibited at Fig.…”

Section: Effect Of Various Ph Toward Lipase Activity Figure 2 Effectmentioning

confidence: 97%

“…Lipases are biocatalyst that plays an important role in a much industrial application such as biopolymer synthesis, biodiesel, drug, food, flavor, oleochemistry, pharmacy, detergent and surfactant [5,6]. Lipases that are used in the industrial or commercial field mostly isolated from bacteria because bacterial lipases are extracellular and easy to handle.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Partial purification and biochemical characterization of extracellular lipase from Azospirillum sp. JG3 bacteria

Lestari¹,

Raharjo²,

Matsjeh³

et al. 2016

AIP Conference Proceedings

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Abstract. An extracellular lipase from Azospirillum sp.JG3 bacteria was partially purified by ammonium sulfate fractionation. Production of crude extract lipase in Nutrient Broth medium fortified with 1 % olive oil as inducer, fractionation of lipase using ammonium sulfate, and characterization of the highest specific activity of lipase fraction have been achieved in this study. The ammonium sulfate lipase fraction which has highest specific activity is F60 with its value is 12,283.33 U/mg protein. The optimum lipase activity of F60 was achieved at 40° C and pH 7.0. The addition of 1.0 mM EDTA strongly decreased its activity. It considered that this lipase was a metalloenzyme. The addition of 1.0 mM Ca 2+ solution showed that this metal ion increased lipase activity, it indicated that Ca 2+ ion is an activator of this lipase. Whereas addition of Mg 2+ , Zn 2+ , Co 2+ , and Cu 2+ metal ions decreased its activity. Effect of surfactants and commercial detergents addition towards lipase activity showed that all treatments decreased the activity.

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Section: Effect Of Various Ph Toward Lipase Activity Figure 2 Effectmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Partial purification and biochemical characterization of extracellular lipase from Azospirillum sp. JG3 bacteria

Lestari¹,

Raharjo²,

Matsjeh³

et al. 2016

AIP Conference Proceedings

View full text Add to dashboard Cite

show abstract

“…Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) form a group of well known hydrolases, whose natural function is the hydrolysis of esters formed from glycerol and long-chain fatty acids. Their enzymology and structure is discussed in several review papers, some of which also provide overviews of the wide range of industrial applications of these enzymes (Bornscheuer et al 2002;Hasan et al 2006). Lipases commercialized by Novozymes A/S (Bagsvaerd, Denmark) under the trade name Resinase A2X were successfully applied at mill scale for the enzymatic control of pitch in mechanical softwood pulping in Japan in the early 1990s (Fujita et al 1991(Fujita et al , 1992Hata et al 1996;Matsukura et al 1990).…”

Section: A Hydrolytic Enzymesmentioning

confidence: 99%

Fungi and Their Enzymes for Pitch Control in the Pulp and Paper Industry

Gutiérrez

Rı́o

Martı́nez

2010

Industrial Applications

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“…Some important lipase producing bacterial genera include Bacillus, Pseudomonas and Burkholderiaetc. Lipase/esterase-producing bacteria have been found in diverse habitats such as soil contaminated with oil, dairy waste, industrial wastes, oil seeds and decaying food, compost heaps, coal tips and hot springs [15,16] .The demand for the biocatalysts with novel and specific properties such as specificity, stability, pH, and temperature is increasing day by day [17,18] . Currently bacterial lipases are of great demand because of potential industrial applications.…”

Section: Introductionmentioning

confidence: 99%