Optimizing the composition of gelatin methacryloyl and hyaluronic acid methacryloyl hydrogels to maximize mechanical and transport properties using response surface methodology

Talaei, Alireza; O’Connell, Cathal; Sayyar, Sepidar; Maher, Malachy; Yue, Zhilian; Choong, Peter; Wallace, Gordon G.

doi:10.1002/jbm.b.35169

Cited by 6 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The R-squared value for the swelling ratio analysis model is 0.898, demonstrating a strong level of correlation. In addition, the Predicted R 2 of 0.829 is in reasonable agreement with the Adjusted R 2 of 0.870, as the difference is less than 0.2 (Table 6) [23,31,32]. Studying the p-values of the terms included in the model (Table 6), it is evident that the factors A, alginate ratio, and C, concentration (crosslinker), have a statistically significant impact on the swelling behavior of the samples.…”

Section: Design Of Experiments 231 Model Summary and Regression Analy...supporting

confidence: 65%

“…The R-squared value for the swelling ratio analysis model is 0.898, demonstrating a strong level of correlation. In addition, the Predicted R 2 of 0.829 is in reasonable agreement with the Adjusted R 2 of 0.870, as the difference is less than 0.2 (Table 6) [23,31,32]. Regarding the analysis of the scaffolds' swelling ratios, the statistical model shows strong significance with a very low p-value (<0.0001) (Table 6).…”

Section: Design Of Experiments 231 Model Summary and Regression Analy...supporting

confidence: 64%

“…The R-squared value of the degradation analysis model is 0.975, indicating a very strong level of correlation. The Predicted R 2 of 0.933 closely aligns with the Adjusted R 2 of 0.963, with a difference of less than 0.2 between them (Table 5) [23,31,32].…”

Section: Design Of Experiments 231 Model Summary and Regression Analy...supporting

confidence: 59%

See 2 more Smart Citations

Alginate–Gelatin Hydrogel Scaffolds; An Optimization of Post-Printing Treatment for Enhanced Degradation and Swelling Behavior

Kaliampakou,

Lagopati,

Pavlatou

et al. 2023

Gels

View full text Add to dashboard Cite

The generation of 3D structures comprises three interlinked phases: material development, the printing process, and post-printing treatment. Numerous factors control all three phases, making the optimization of the entire process a challenging task. Until now, the state of the art has mainly focused on optimizing material processability and calibration of the printing process. However, after the successful Direct Ink Writing (DIW) of a hydrogel scaffold, the post-printing stage holds equal importance, as this allows for the treatment of the structure to ensure the preservation of its structural integrity for a duration that is sufficient to enable successful cell attachment and proliferation before undergoing degradation. Despite this stage’s pivotal role, there is a lack of extensive literature covering its optimization. By studying the crosslinking factors and leveling the post-treatment settings of alginate–gelatin hydrogel, this study proposes a method to enhance scaffolds’ degradation without compromising the targeted swelling behavior. It introduces an experimental design implementing the Response Surface Methodology (RSM) Design of Experiments (DoE), which elucidated the key parameters influencing scaffold degradation and swelling, and established an alginate ratio of 8% and being immersed for 15 min in 0.248 M CaCl2 as the optimal level configuration that generates a solution of 0.964 desirability, reaching a degradation time of 19.654 days and the swelling ratio of 50.00%.

show abstract

Section: Design Of Experiments 231 Model Summary and Regression Analy...supporting

confidence: 65%

“…The R-squared value for the swelling ratio analysis model is 0.898, demonstrating a strong level of correlation. In addition, the Predicted R 2 of 0.829 is in reasonable agreement with the Adjusted R 2 of 0.870, as the difference is less than 0.2 (Table 6) [23,31,32]. Regarding the analysis of the scaffolds' swelling ratios, the statistical model shows strong significance with a very low p-value (<0.0001) (Table 6).…”

Section: Design Of Experiments 231 Model Summary and Regression Analy...supporting

confidence: 64%

See 1 more Smart Citation

Alginate–Gelatin Hydrogel Scaffolds; An Optimization of Post-Printing Treatment for Enhanced Degradation and Swelling Behavior

Kaliampakou,

Lagopati,

Pavlatou

et al. 2023

Gels

View full text Add to dashboard Cite

show abstract

“…GelMA was the biomaterial used as the scaffold to grow cells in 3D because of its versatility in tissue engineering and 3D printing. GelMA is an engineered gelatin-based material with a methacryloyl functionalization that enables cross-linking and provides mechanical properties to be used as a scaffold for 3D cultures. , The concentrations of GelMA were optimized to facilitate the optimal growth of the cultured cells within predefined time intervals. A range of concentrations, specifically low (3% w/v ratio), medium (5.5–6%), and high (10%) were systematically tested (Figure S5).…”

Section: Resultsmentioning

confidence: 99%

High-Throughput 3D-Printed Model of the Feto-Maternal Interface for the Discovery and Development of Preterm Birth Therapies

Cherukuri,

Kammala,

Thomas

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Spontaneous preterm birth (PTB) affects around 11% of births, posing significant risks to neonatal health due to the inflammation at the fetal−maternal interface (FMi). This inflammation disrupts immune tolerance during pregnancy, often leading to PTB. While organ-on-a-chip (OOC) devices effectively mimic the physiology, pathophysiology, and responses of FMi, their relatively low throughput limits their utility in high-throughput testing applications. To overcome this, we developed a threedimensional (3D)-printed model that fits in a well of a 96-well plate and can be mass-produced while also accurately replicating FMi, enabling efficient screening of drugs targeting FMi inflammation. Our model features two cell culture chambers (maternal and fetal cells) interlinked via an array of microfluidic channels. It was thoroughly validated, ensuring cell viability, metabolic activity, and cellspecific markers. The maternal chamber was exposed to lipopolysaccharides (LPS) to induce an inflammatory state, and proinflammatory cytokines in the culture supernatant were quantified. Furthermore, the efficacy of anti-inflammatory inhibitors in mitigating LPS-induced inflammation was investigated. Results demonstrated that our model supports robust cell growth, maintains viability, and accurately mimics PTB-associated inflammation. This high-throughput 3D-printed model offers a versatile platform for drug screening, promising advancements in drug discovery and PTB prevention.

show abstract

“…For example, they are promising materials for development of novel energy storage devices, 1,2 catalysts, 3 food products, 4 and so forth. Hydrogel materials are especially widely used in the development of materials for biomedical application because hydrogels have a unique combination of properties that make it possible to create innovative biomaterials with improved pharmacological and consumer characteristics 5–8 . Due to the three‐dimensional framework, hydrogels can exhibit mechanical properties of solids, such as ability to deformation, retention of shape, lack of fluidity.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and study of organo‐modified silica based hydrogels: Rheological properties and drug release kinetics

Parfenyuk,

Dolinina,

Kraev

2024

J Biomed Mater Res

View full text Add to dashboard Cite

The method of synthesis of unmodified and organo‐modified silica hydrogels and their composites with orotic acid as a model drug was developed. The hydrogels had a pH of 6.5–7.8. The particulate nature and highly porous structures of the hydrogel materials were revealed using scanning electron and optical microscopy methods. The content of aqueous phase in the hydrogels was 99% or more. In order to evaluate the possibility of their application as a basis for development of novel soft drug formulations and cosmetic compositions, rheological properties of the hydrogels and in vitro release kinetics of the drug were studied. The effects of synthesis conditions (increasing concentration of catalyst of silica sol formation, drug loading) and the silica matrix modification with various organic groups on the indicated properties were investigated. It was found that all synthesized hydrogels exhibited pseudoplasticity, thixotropy and controlled release of the drug, which are important for their potential application. However, in general, the indicated effects led to worsening the properties of the hydrogel materials in comparison with the unmodified silica hydrogels.

show abstract

Optimizing the composition of gelatin methacryloyl and hyaluronic acid methacryloyl hydrogels to maximize mechanical and transport properties using response surface methodology

Abstract: Hydrogel materials are promising candidates in cartilage tissue engineering as they provide a 3D porous environment for cell proliferation and the development of new cartilage tissue. Both the mechanical and transport properties of hydrogel scaffolds

Cited by 6 publications

References 45 publications

Alginate–Gelatin Hydrogel Scaffolds; An Optimization of Post-Printing Treatment for Enhanced Degradation and Swelling Behavior

Alginate–Gelatin Hydrogel Scaffolds; An Optimization of Post-Printing Treatment for Enhanced Degradation and Swelling Behavior

High-Throughput 3D-Printed Model of the Feto-Maternal Interface for the Discovery and Development of Preterm Birth Therapies

Synthesis and study of organo‐modified silica based hydrogels: Rheological properties and drug release kinetics

Contact Info

Product

Resources

About