A new genotype of spineless cactus is being used in the diets of dairy cattle that are raised in semiarid regions. However, little is known about its nutritional value. This study aimed to evaluate the effects of replacing Miúda (Nopalea cochenillifera Salm Dyck) with Orelha de Elefante Mexicana (Opuntia stricta [Haw.] Haw.) spineless cactus, on nutrient intake and digestibility, milk yield and composition, feeding behaviour, microbial protein synthesis, nitrogen balance, and ruminal and blood parameters of dairy cows. Ten Girolando cows, 500 ± 51.6 kg bodyweight, were distributed in a double Latin square design 5 x 5. The treatments consisted of replacement levels of Miúda (MIU) by Orelha de Elefante Mexicana (OEM) at 0, 25, 50, 75, and 100%. The intake and digestibility of dry matter (DM) (14.38-12.95 kg d ) and total digestible nutrients (TDN) (9.38-7.92 kg d ) increased linearly. Thus, OEM could replace 100% MIU in diets with 48% of spineless cactus, for crossbred lactating cows with 12.5 kg d -1 milk yield. Therefore, OEM is a viable new option for producing milk in smallholder livestock systems in semi-arid regions. ______________________________________________________________________________________