Optimum conditions for the enzymatic hydrolysis of citron waste juice using response surface methodology (RSM)

Lim, Ji Young; Yoon, Hyang-Sik; Kim, Kwang-Yup; Kim, Ki-Sik; Noh, Jae Goan; Song, In Gyu

doi:10.1007/s10068-010-0162-3

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…The most frequently present components in C. medica are limonin and nomilin, which are responsible for the bitter taste of the genus Citrus. Lim et al [ 56 ] investigated the optimum conditions for the enzymatic hydrolysis of citron waste juice using the response surface methodology: the highest contents of limonin and nomilin were 3.49 mg/100 g (extraction conditions: pH 4.51, temperature 50.30 °C, time 48.34 min, and 0.21% yield) and 1.56 mg/100 g (extraction conditions: pH 4.59, temperature 50.08 °C, time 66.07 min, and 0.30%), respectively. The terpenes identified in C. medica are shown in Table 4 .…”

Section: Resultsmentioning

confidence: 99%

An Insight into Citrus medica Linn.: A Systematic Review on Phytochemical Profile and Biological Activities

Benedetto,

Carlucci,

Faraone

et al. 2023

Plants

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Plant species are a reservoir of natural compounds that can potentially be used to treat different diseases. Citrus medica Linn. belonging to the Rutaceae family, has been used for centuries in medicine for its antioxidant, anti-inflammatory, antimicrobial, antiviral, and antihyperglycemic properties. These activities are ascribable not only to the presence of health-promoting macronutrients and micronutrients, such as carbohydrates, minerals, amino acids, and vitamins, but also to specialized metabolites, such as flavonoids (apigenin, hesperetin, hesperidin, naringin, naringenin, rutin, quercetin, and diosmin), coumarins (citropten, scoparone, and bergapten), terpenes (limonene, γ-terpinene, limonin, and nomilin), and phenolic acids (p-coumaric acid, trans-ferulic acid, and chlorogenic acid). In recent years, particular attention has been focused on the antioxidant, anti-inflammatory, antimicrobial activity, antidiabetic, anticancer, and neuroprotective activity of C. medica. However, although many studies have reported this species’ chemical and biological properties, the literature has never been analyzed via a systematic approach. For this reason, using PubMed and Scopus as databases, we performed a systematic review of C. medica’s chemical composition and biological properties to inspire new research approaches and increase its curative application.

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Section: Resultsmentioning

confidence: 99%

An Insight into Citrus medica Linn.: A Systematic Review on Phytochemical Profile and Biological Activities

Benedetto,

Carlucci,

Faraone

et al. 2023

Plants

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“…When a solution of DPPH is mixed with that of a substrate (AH) that can donate a hydrogen atom, then this gives rise to the reduced form with the loss of this violet color, characterized by an absorption band in ethanol solution centered at about 517 nm. The DPPH radical-scavenging capacity of goat placenta peptides was carried out using a previously method ( Bey et al ., 2013 ; Lim et al ., 2010 ; Zhang et al ., 2011b ) with minor modification. Briefly, sample (0.5 mL) was mixed with 2.5 mL of distilled water and 3 mL 0.1 mM DPPH in ethanol and kept for 30 min in the dark at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of Antioxidant Peptides from Fermented Goat Placenta

Hou

Zhou

Liu

et al. 2014

Korean Journal for Food Science of Animal Resources

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The goat placenta was fermented by Bacillus subtilis and the optimal fermentation parameters of strongest antioxidant capacity of peptides were obtained using response surface methodology (RSM). The effects of fermentation time, initial pH value and glucose content on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of the goat peptides were well fitted to a quadric equation with high determination coefficients. According to the data analysis of design expert, the strongest DPPH radical scavenging capacity value was obtained with the following conditions: content of glucose was 2.23%, initial pH value was 7.00 and fermentation time was 32.15 h. The DPPH radical scavenging capacity commonly referring antioxidant activity showed a concentration dependency and increased with increasing peptide concentration. The effects of temperature and pH were assessed to determine the stability of antioxidant peptides prepared from goat placenta. Antioxidant peptides showed good stabilities when temperature was lower than 70℃. However, the antioxidant peptides lost antioxidant activities rapidly under alkaline and excessive acid condition. Ultrafiltration technique was performed to separate fermentation broth with different Mw (molecular weight). It was found that peptides in the range of < 3 KDa mainly accounted for the antioxidant activities.

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“…These constitute about 40% of the total mass. The majority of these wastes are often not properly disposed and they constitute huge environmental pollution [3]. Environments dumped with fruit peels provide a good site for the proliferation of microorganisms including (pathogenic) fungi.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Proximate Contents and Potentials of Musa sapientum and Persea americana Fruits Peel as Media for the Growth of Medically Important Fungi

Ekwe

Ekwebelem

Eze

2020

JAMB

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Aim: The present study was designed to formulate growth media for medically important fungi using banana and avocado fruits peel. Methodology: The peels were obtained from fresh banana and avocado fruits and dried into crisps at 100oC using hot air oven, pulverised into powder using Hammer mill machine and sieved into fine powder using 1 mm sieve size. Pure isolates of Aspergillus flavus, Aspergillus fumigatus and Cladosporium sp. were obtained from the Diagnostic Laboratory of the Department of Microbiology, University of Nigeria, Nsukka and used for the study. Two different media, Banana Peel Agar (BPA) and Avocado Peel Agar (APA) were formulated. 2 g of each fruit peel powder was introduced into separate conical flasks, 1g of agar powder was added as solidifying agent and varied glucose concentrations (20%, 10%, 5% and 0%) was used as nutrient (carbon source) supplement. The prepared media was sterilized by autoclaving at 121°C for 15 minutes. The test organisms were inoculated aseptically onto the formulated media and incubated for 3 days at 37oC. Fungal growth was visually observed. For comparative analysis, Sabouraud dextrose agar (SDA) was used as control. Results: The formulated media supported the growth of all the test organisms, although at varying degrees. BPA showed more suitability than APA, with the growth of the test organisms on BPA favourably comparable to that on SDA. Proximate analysis of the fruit peels indicated that the moisture, ash, fat, crude fibre, crude protein and carbohydrate contents of avocado peel were 7.35%, 14.65%, 0.10%, 9.75%, 6.57% and 61.58% respectively and 5.80%, 6.00%, 0.45%, 5.35%, 10.95% and 71.45% respectively for banana peel. Conclusion: Banana and avocado fruits peel can be utilized as alternative materials in the formulation of culture media for the in vitro cultivation of fungi for medical and research purposes.

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Optimum conditions for the enzymatic hydrolysis of citron waste juice using response surface methodology (RSM)

Cited by 10 publications

References 13 publications

An Insight into Citrus medica Linn.: A Systematic Review on Phytochemical Profile and Biological Activities

An Insight into Citrus medica Linn.: A Systematic Review on Phytochemical Profile and Biological Activities

Preparation and Characterization of Antioxidant Peptides from Fermented Goat Placenta

Studies on the Proximate Contents and Potentials of Musa sapientum and Persea americana Fruits Peel as Media for the Growth of Medically Important Fungi

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