Optogenetic control of focal adhesion kinase signaling

Hörner, Maximilian; Chatelle, Claire; Mühlhäuser, Wignand W. D.; Stocker, David R.; Coats, Michael T.; Weber, Wilfried; Radziwill, Gerald

doi:10.1016/j.cellsig.2017.10.012

Cited by 11 publications

(3 citation statements)

References 35 publications

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“…4.4. A related study optogenetically targeted FAK downstream of the integrin receptor. 330 FAK was C-terminally connected to At CRY2 to allow for BL-induced clustering, resultant autophosphorylation and concomitant activation of this kinase. The BL-activated FAK variant is suited to study the lower branches of the signaling network without upstream input by the integrin receptors.…”

Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

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Blue-Light Receptors for Optogenetics

2018

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Sensory photoreceptors underpin light-dependent adaptations of organismal physiology, development, and behavior in nature. Adapted for optogenetics, sensory photoreceptors become genetically encoded actuators and reporters to enable the noninvasive, spatiotemporally accurate and reversible control by light of cellular processes. Rooted in a mechanistic understanding of natural photoreceptors, artificial photoreceptors with customized light-gated function have been engineered that greatly expand the scope of optogenetics beyond the original application of light-controlled ion flow. As we survey presently, UV/blue-light-sensitive photoreceptors have particularly allowed optogenetics to transcend its initial neuroscience applications by unlocking numerous additional cellular processes and parameters for optogenetic intervention, including gene expression, DNA recombination, subcellular localization, cytoskeleton dynamics, intracellular protein stability, signal transduction cascades, apoptosis, and enzyme activity. The engineering of novel photoreceptors benefits from powerful and reusable design strategies, most importantly light-dependent protein association and (un)folding reactions. Additionally, modified versions of these same sensory photoreceptors serve as fluorescent proteins and generators of singlet oxygen, thereby further enriching the optogenetic toolkit. The available and upcoming UV/blue-light-sensitive actuators and reporters enable the detailed and quantitative interrogation of cellular signal networks and processes in increasingly more precise and illuminating manners.

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Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

“…section ). A related study optogenetically targeted FAK downstream of the integrin receptor . FAK was C-terminally connected to At CRY2 to allow for BL-induced clustering, resultant autophosphorylation, and concomitant activation of this kinase.…”

Section: Photoreceptors As Actuators In Optogeneticsmentioning

confidence: 99%

Blue-Light Receptors for Optogenetics

2018

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“…Full stimulation of FAK activity involves an SRC-dependent positive feedback loop. OptoFAK represents a single component, blue light-activatable FAK based on FAK-CRY2 oligomerization ( Figure 4 B) [ 83 ]. Light-induced FAK oligomers recruited to focal adhesion sites and mediated phosphorylation of SRC, paxillin, and p130CAS, components of the integrin signaling.…”

Section: Light Control Of Integrin Signalingmentioning

confidence: 99%

Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways

Kramer

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Weber

et al. 2021

IJMS

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Biological signals are sensed by their respective receptors and are transduced and processed by a sophisticated intracellular signaling network leading to a signal-specific cellular response. Thereby, the response to the signal depends on the strength, the frequency, and the duration of the stimulus as well as on the subcellular signal progression. Optogenetic tools are based on genetically encoded light-sensing proteins facilitating the precise spatiotemporal control of signal transduction pathways and cell fate decisions in the absence of natural ligands. In this review, we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins. We briefly discuss the most common optogenetic switches and their mode of action. The main part deals with the engineering and application of optogenetic tools for the control of transmembrane receptors including receptor tyrosine kinases, the T cell receptor and integrins, and their effector proteins. We also address the hallmarks of optogenetics, the spatial and temporal control of signaling events.

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Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion

2019

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The dynamic and spatiotemporal control of integrin‐mediated cell adhesion to RGD motifs in its extracellular matrix (ECM) is important for understating cell biology and biomedical applications because cell adhesion fundamentally regulates cellular behavior. Herein, the first photoswitchable synthetic ECM protein, Photo‐ECM, based on the blue light switchable protein LOV2 is engineered. The Photo‐ECM protein includes a RGD sequence, which is hidden in the folded LOV2 protein structure in the dark and is exposed under blue light so that integrins can bind and cells can adhere. The switchable presentation of the RGD motif allows to reversibly mediate and modulate integrin‐based cell adhesions using noninvasive blue light. With this protein cell adhesions in live cells could be reversed and the dynamics at the cellular level is observed. Hence, the Photo‐ECM opens a new possibility to investigate the spatiotemporal regulation of cell adhesions in cell biology and is the first step toward a genetically encoded and light‐responsive ECM.

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Optogenetic control of focal adhesion kinase signaling

Cited by 11 publications

References 35 publications

Blue-Light Receptors for Optogenetics

Blue-Light Receptors for Optogenetics

Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways

Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion

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