Oral Bone Tissue Engineering: Advanced Biomaterials for Cell Adhesion, Proliferation and Differentiation

Roi, Alexandra; Ardelean, Lavinia; Roi, Ciprian Ioan; Boia, Eugen-Radu; Boia, Simina; Rusu, Laura-Cristina

doi:10.3390/ma12142296

Cited by 30 publications

(27 citation statements)

References 112 publications

(115 reference statements)

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“…For a successful osteoconduction, the 3D structure of a scaffold should support cell penetration and proliferation as well as neovascularization, enabling an adequate diffusion of nutrients to the new cells and preventing the failure of bone repair [4,[34][35][36][37]. Moreover, scaffolds should present interconnected pores of sizes and morphologies adequate to ensure the diffusion of waste products out of the scaffold without interfering with other surrounding tissues [34].…”

Section: Discussionmentioning

confidence: 99%

Physical and Mechanical Properties of Composite Scaffolds with or without Collagen Impregnation

et al. 2019

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This in vitro study aimed at evaluating the physical and mechanical properties of newly developed scaffolds of poly (lactic-co-glycolic acid) (PLGA) and biphasic ceramic (Hydroxyapatite HA + beta-tricalciumphosphate β-TCP) with or without collagen impregnation to be used for bone regeneration in the oral and maxillofacial district. Solvent casting and particle leaching techniques were used to produce the scaffolds, which were then divided into six groups according to PLGA/HA + β-TCP ratio and impregnation with collagen: G1 (50/50) + collagen; G2 (60/40) + collagen; G3 (40/60) + collagen; G4 (50/50); G5 (60/40); G6 (40/60). As control group, inorganic xenogenous bone was used. Structure and porosity were evaluated by scanning electron microscopy, and a chemical analysis was performed through an energy-dispersive spectrometer. Moreover, to evaluate the hydrophilicity of the samples, a wettability test was conceived, and finally, mechanical properties were examined by a compression test. High porosity and interconnectivity, resulting in a large surface area and great fluid retention capacity, were presented by the PLGA/HA + β-TCP scaffolds. In the composite groups, collagen increased the wettability and the mechanical resistance, although the latter was not statistically affected by the percentage of HA + β-TCP added. Further in vitro and in vivo studies are needed for a deeper understanding of the influence of collagen on the biological behavior of the developed composite materials and their potential, namely biocompatibility and bioactivity, for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Physical and Mechanical Properties of Composite Scaffolds with or without Collagen Impregnation

et al. 2019

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“…If gingivitis is not treated in time, the inflammation can spread from gingival to the periodontal membrane and alveolar bone, thus forming the periodontitis. Due to no obvious conscious symptoms and easy to be ignored at initial stage, once the symptoms are more serious, treatment of such pathology is a great challenge for the clinician ( Cortellini and Tonetti, 2015 ; Larsson et al, 2015 ; Kinane et al, 2017 ; Roi et al, 2019 ; Xu et al, 2020a,b ). In general, reducing the inflammation is a key to control the infection for periodontal therapy, but traditional mechanical treatments like scaling and root planin are closely connected with the wound repair.…”

Section: Cs-based Hydrogelsmentioning

confidence: 99%

Recent Advances of Chitosan-Based Injectable Hydrogels for Bone and Dental Tissue Regeneration

Tang

Tan

Zeng

et al. 2020

Front. Bioeng. Biotechnol.

113

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Traditional strategies of bone repair include autografts, allografts and surgical reconstructions, but they may bring about potential hazard of donor site morbidity, rejection, risk of disease transmission and repetitive surgery. Bone tissue engineering (BTE) is a multidisciplinary field that offers promising substitutes in biopharmaceutical applications, and chitosan (CS)-based bone reconstructions can be a potential candidate in regenerative tissue fields owing to its low immunogenicity, biodegradability, bioresorbable features, low-cost and economic nature. Formulations of CS-based injectable hydrogels with thermo/pH-response are advantageous in terms of their highwater imbibing capability, minimal invasiveness, porous networks, and ability to mold perfectly into an irregular defect. Additionally, CS combined with other naturally-derived or synthetic polymers and bioactive agents has proven to be an effective alternative to autologous bone and dental grafts. In this review, we will highlight the current progress in the development of preparation methods, physicochemical properties and applications of CS-based injectable hydrogels and their perspectives in bone and dental regeneration. We believe this review is intended as starting point and inspiration for future research effort to develop the next generation of tissue-engineering scaffold materials.

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“…It is essential for orthopedic and dental implants to establish a robust implant–bone interface. Bone cells must adhere, spread, proliferate, and differentiate for successful tissue integration [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

DNA Polyelectrolyte Multilayer Coatings Are Antifouling and Promote Mammalian Cell Adhesion

et al. 2021

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The ability of bacteria to adhere to and form biofilms on implant surfaces is the primary cause of implant failure. Implant-associated infections are difficult to treat, as the biofilm mode of growth protects microorganisms from the host’s immune response and antibiotics. Therefore, modifications of implant surfaces that can prevent or delay bacterial adhesion and biofilm formation are highly desired. In addition, the attachment and spreading of bone cells are required for successful tissue integration in orthopedic and dental applications. We propose that polyanionic DNA with a negatively charged phosphate backbone could provide a dual function to repel bacterial adhesion and support host tissue cell attachment. To this end, we developed polyelectrolyte multilayer coatings using chitosan (CS) and DNA on biomaterial surfaces via a layer-by-layer technique. The assembly of these coatings was characterized. Further, we evaluated staphylococcal adhesion and biofilm growth on the coatings as well as cytotoxicity for osteoblast-like cells (SaOS-2 cells), and we correlated these to the layer structure. The CS-DNA multilayer coatings impaired the biofilm formation of Staphylococcus by ~90% on both PMMA and titanium surfaces. The presence of cationic CS as the top layer did not hinder the bacteria-repelling property of the DNA in the coating. The CS-DNA multilayer coatings demonstrated no cytotoxic effect on SaOS-2 cells. Thus, DNA polyelectrolyte multilayer coatings could reduce infection risk while promoting host tissue cell attachment on medical implants.

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Oral Bone Tissue Engineering: Advanced Biomaterials for Cell Adhesion, Proliferation and Differentiation

Cited by 30 publications

References 112 publications

Physical and Mechanical Properties of Composite Scaffolds with or without Collagen Impregnation

Physical and Mechanical Properties of Composite Scaffolds with or without Collagen Impregnation

Recent Advances of Chitosan-Based Injectable Hydrogels for Bone and Dental Tissue Regeneration

DNA Polyelectrolyte Multilayer Coatings Are Antifouling and Promote Mammalian Cell Adhesion

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