Oral colonization by more than one clonal type of mutans streptococcus in children with nursing-bottle dental caries

Alaluusua, Satu; Mättö, Jaana; Grönroos, Lisa; Innilä, S.; Torkko, Heini; Asikainen, Sirkka; Jousimies‐Somer, Hannele; Saarela, Maria

doi:10.1016/0003-9969(95)00111-5

Cited by 106 publications

(128 citation statements)

References 21 publications

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“…This finding was contrary to the results obtained by Kreulen et al (1997), who showed a negative relationship between the caries activity and genotype diversity. However, the present result was in agreement with earlier reports (Hirose et al 1993;Alaluusua et al 1996;Emanuelsson et al 2003;Napimoga et al 2004). It has been observed that the subjects harbor one to five distinct genotypes of S. mutans at different ages (Alallusuua et al 1996;Emmanuelsson et al 1998;Grönroos et al 1998;Klein et al 2004;Mattos-Graner et al 2001;Caulfield and Walker 1989;Kulkarni et al 1989;Kreulen 1997;Napimoga et al 2004).…”

Section: Discussionsupporting

confidence: 93%

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals

Braga

Piovesan

Valarini

et al. 2013

Braz. arch. biol. technol.

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Section: Discussionsupporting

confidence: 93%

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals

Braga

Piovesan

Valarini

et al. 2013

Braz. arch. biol. technol.

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“…Therefore, some studies have shown that the oral cavity may present distinct genotypes of S. mutans, not only in saliva but also in dental biofilm (11,12). Additionally, under frequent sucrose exposure, clinical findings suggest that there was an increase in genotypic diversity of S. mutans in the oral cavity (13). These genotypes could present distinct virulence capacities and different abilities of producing EPS (14).…”

Section: Introductionmentioning

confidence: 99%

Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure

et al. 2007

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In situ dental biofilm composition under sugar exposure is well known, but sugar effect on the genotypic diversity of S. mutans in dental biofilm has not been explored. This study evaluated S. mutans genotypic diversity in dental biofilm formed in situ under frequent exposure to sucrose and its monosaccharide constituents (glucose and fructose). Saliva of 7 volunteers was collected for isolation of S. mutans and the same volunteers wore intraoral palatal appliances, containing enamel slabs, which were submitted to the following treatments: distilled and deionized water (negative control), 10% glucose + 10% fructose (fermentable carbohydrates) solution or 20% sucrose (fermentable and EPS inductor) solution, 8x/day. After 3, 7 and 14 days, the biofilms were colleted and S. mutans colonies were isolated. Arbitrarily primed polymerase chain reaction (AP-PCR) of S. mutans showed that salivary genotypes were also detected in almost all biofilm samples, independently of the treatment, and seemed to reflect those genotypes present at higher proportion in biofilms. In addition to the salivary genotypes, others were found in biofilms but in lower proportions and were distinct among treatment. The data suggest that the in situ model seems to be useful to evaluate genotypic diversity of S. mutans, but, under the tested conditions, it was not possible to clearly show that specific genotypes were selected in the biofilm due to the stress induced by sucrose metabolism or simple fermentation of its monosaccharides.

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“…Studies using phenotyping and/or genotyping methods suggest that the mother is the major primary source of infection for children who carry S. mutans and/or S. sobrinus strains (1,7,8,18,21,35). However, detection of genotypes that are not found in children's mothers or other family members indicates that S. mutans and/or S. sobrinus may also be acquired from other sources (7,8,18,28).…”

mentioning

confidence: 99%

Longitudinal Study of Transmission, Diversity, and Stability of Streptococcus mutans and Streptococcus sobrinus Genotypes in Brazilian Nursery Children

et al. 2004

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The aim of this study was to perform a follow-up evaluation of the Streptococcus mutans and Streptococcus sobrinus colonization profile of children's oral cavities, which included the pattern of vertical transmission from mother to child, genotypic diversity, and stability of the strains. The subjects were 16 mother-child pairs, who were monitored for 20 months. Samples of saliva, tongue dorsum, alveolar ridge mucosa, and dental plaque from the children were collected bimonthly. Saliva samples from the mothers were also collected. After isolation and identification, the arbitrarily primed PCR method was performed for the genotypic characterization of S. mutans (968 isolates) and S. sobrinus (111 isolates). At the time the strains were acquired, the children harbored one to four distinct genotypes of S. mutans and only one genotype of S. sobrinus. Although S. mutans prevalence and genotypic diversity were greater than those of S. sobrinus, the presence of matching genotypes of S. mutans and S. sobrinus was similar (in 81.25 and 83.33% of mother-child pairs, respectively), suggesting vertical transmission for both species. This longitudinal study showed an increase in genotypic diversity of S. mutans in the oral cavity during the follow-up period: most of the initially acquired genotypes persisted, normally those genotypes transmitted by the mother, and some were lost during follow-up; new strains were also acquired. In conclusion, S. mutans and S. sobrinus genotypes acquired from maternal or alternative sources may show effective persistence in the oral cavity and/or transitory detection in the children's mouths, reflecting the continuous development of oral microbiota in children.

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Oral colonization by more than one clonal type of mutans streptococcus in children with nursing-bottle dental caries

Cited by 106 publications

References 21 publications

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals

Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure

Longitudinal Study of Transmission, Diversity, and Stability of Streptococcus mutans and Streptococcus sobrinus Genotypes in Brazilian Nursery Children

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