2020
DOI: 10.1101/2020.10.23.351817
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Oral immunization of broilers with chitosan nano-encapsulated extracellular and cell wall proteins of necrotic enteritis-inducedClostridium perfringens

Abstract: Currently, there is no commercial vaccine to control  Clostridium perfringens  (CP) or necrotic enteritis – a clinically and economically devastating disease. Two chitosan-nanoparticle encapsulated CP (CNP-CP) vaccines using extracellular proteins (ECP) and cell wall proteins (CWP) were synthesized: a CNP-CP ECP + CWP vaccine (SC vaccine) and a CNP-CP ECP + CWP surface-adsorbed CWP vaccine (SCC vaccine). The experiment comprised a complete randomized design of 3 treatments replicated 5 times: SC, SCC and non… Show more

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Cited by 4 publications
(2 citation statements)
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“…At D35 of age, the NE challenge significantly decreased the concentration of IgA in bile, decreased the ratio of CD4+ and CD8+ T cells in the spleen, decreased expression of inflammatory and anti-inflammatory cytokines such as IL-10, IFN-γ, and iNOS in the jejunum and decreased the expression of IL-10 in CT. The decrease in IgA due to challenges during late infection was documented in numerous studies ( Wang et al, 2017 ; Ramadan et al, 2020 ; Zhao et al, 2022 ). Also, synbiotic supplementation increased the percentage of Tregs in the spleen and decreased the expression of IL-1β and IFN-γ mRNA in the challenged bird.…”
Section: Discussionmentioning
confidence: 87%
“…At D35 of age, the NE challenge significantly decreased the concentration of IgA in bile, decreased the ratio of CD4+ and CD8+ T cells in the spleen, decreased expression of inflammatory and anti-inflammatory cytokines such as IL-10, IFN-γ, and iNOS in the jejunum and decreased the expression of IL-10 in CT. The decrease in IgA due to challenges during late infection was documented in numerous studies ( Wang et al, 2017 ; Ramadan et al, 2020 ; Zhao et al, 2022 ). Also, synbiotic supplementation increased the percentage of Tregs in the spleen and decreased the expression of IL-1β and IFN-γ mRNA in the challenged bird.…”
Section: Discussionmentioning
confidence: 87%
“…The effect of chitosan nanoparticles has been studied on the efficiency of clostridial vaccines. The vaccine containing chitosan could stimulate humoral immunity 2-3 times higher than the nonchitosan toxoid vaccinated rabbits (Fathi Najafi et al 2020) or non-immunized chickens (Ramadan et al 2020). The results of nanovaccines should be compared with other vaccines currently used and their protective effects need to be evaluated in the field, to confirm their applicability.…”
Section: Nanovaccinesmentioning
confidence: 99%