Oral immunization with recombinant Lactobacillus casei expressing flaB confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio

Kong, Yuanyuan; Kang, Yuan-Huan; Tian, Jingkui; Zhang, Dong-Xing; Zhang, Lei; Tao, LuoTao; Wu, Tianlong; Liu, Ying; Wang, Guiqin; Shan, Xiaofeng

doi:10.1016/j.fsi.2019.01.032

Cited by 39 publications

(20 citation statements)

References 27 publications

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“…2012). Kong et al (2019) constructed two recombinant Lactobacillus casei (surface‐displayed or secretory) expressing the flaB of A. veronii and evaluated the effect of immune responses in common carp. The result showed that recombinant L. casei may effectively induce humoral immunity and increase the serum immunological index, and surface‐displayed system could induce even higher levels and received 66.7% survival rates than the controls after challenge with the pathogen A. veronii (Kong et al .…”

Section: Application In Aquaculturementioning

confidence: 99%

“…The result showed that recombinant L. casei may effectively induce humoral immunity and increase the serum immunological index, and surface‐displayed system could induce even higher levels and received 66.7% survival rates than the controls after challenge with the pathogen A. veronii (Kong et al . 2019). Similarly, Zhang et al (2018) generated two recombinant Lactobacillus casei (surface‐displayed or secretory) expressing the OmpAI of A. veronii .…”

Section: Application In Aquaculturementioning

confidence: 99%

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Surface display system for probiotics and its application in aquaculture

Yao

Yang

Gao

et al. 2020

Reviews in Aquaculture

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Aquaculture is a fast‐growing and rapidly expanding industry in the world. Probiotics are widely used in aquaculture and have provided benefits to aquatic animal health, and it is also a promising alternative to antibiotics for control of fish diseases. With the development of biotechnology, new expression systems and novel techniques for the surface display of heterologous proteins in surface of probiotics cells have been developed. This review provides an overview of the systems and strategies for displaying functional proteins on the surface of probiotics commonly used in aquaculture, which are Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria represented by Lactococcus lactis and yeast. Their applications in aquaculture especially for oral vaccine development afforded by this technology and prospects and challenges associated with this technology are also highlighted.

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Section: Application In Aquaculturementioning

confidence: 99%

Section: Application In Aquaculturementioning

confidence: 99%

Surface display system for probiotics and its application in aquaculture

Yao

Yang

Gao

et al. 2020

Reviews in Aquaculture

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“…They can recognize denatured proteins, assist protein repair, assist immunity, reduce oxidative damage and play a protective role (Li, Liu, et al, 2019; Roberts, Agius, Saliba, Bossier, & Sung, 2010). Interferon‐γ can effectively block the invasion of pathogens, combined with a variety of immune enzymes to resist virus infection (Kong et al., 2019). IGF‐1 is a necessary active protein polypeptide in the physiological process of growth hormone production, and it has the laudatory name of "growth hormone medium"(Chen et al., 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Amylase (AMS) activity (Yu, Li, Zhu, Li, & Wu, 2020), lipase (LPS) activity (Long, Yang, Li, Guan, & Wu, 2015), protease (PRS) activity (Xu et al., 2013), cellulase (CES) activity (Yu, Wu, Zheng, et al, 2020); lysozyme (LZM) activity (Chen, Ren, Li, & Xia, 2018), complement C3, C4 (Li, Zhu, Tian, Liu, & Wang, 2019) and alkaline phosphatase (AKP) activity (Li, Li, et al, 2019); total superoxide dismutase (T‐SOD) activity (Chen, Ren, Wang, Xia, & Li, 2018), total antioxidant capacity (T‐AOC) activity (Yu, Li, Zhu, Li, Duan, et al, 2020), catalase (CAT) activity (Yu, Zheng, Du, Li, et al, 2020), ascorbic acid (ASA) activity (Feng et al., 2016), glutathione peroxidase (GSH‐PX) activity (Li et al., 2020) activity and malondialdehyde (MDA) content (Yin et al., 2018); ELISA Kit: heat shock proteins 70 (HSP70), heat shock proteins 90 (HSP90; Li, Liu, Xia, Wang, & Zhang, 2019), insulin‐like growth factor I (IGF‐1; Chen et al., 2016) and interferon‐γ (IFN‐γ; Kong et al., 2019) were determined using commercial assay kits (Nanjing Jiancheng Bioengineering Institute).…”

Section: Methodsmentioning

confidence: 99%

Influence of differential protein levels of feed on growth, copper‐induced immune response and oxidative stress of Rhynchocypris lagowski in a biofloc‐based system

Huang

et al. 2020

Aquacult. Nutr.

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This study compared the influences of differential protein levels of feed on growth, copper-induced digestive enzyme activity, immune response, oxidative stress and Nrf2 signalling molecules genes expression of Rhynchocypris lagowski in a bioflocbased system and traditional autotrophic system. Four hundred and fifty R. lagowski were fed four experimental dietary containing graded levels of protein from 25% to 37% (referred as biofloc technology [BFT]25, BFT29, BFT33 and BFT37, respectively) in a biofloc-based system, and 37% crude protein level (control group) were cultured in an autotrophic system in 8 weeks. At the end of the trial, there was an improvement of 50.35%-61.64% in terms of growth performance (weight gain rate); 0.25-0.53 (U/mg prot) amylase; 6.9-8.02 (U/ml) lysozyme (LZM); and 7.98-8.12 (U/ ml) total superoxide dismutase (T-SOD) in R. lagowski cultured in BFT37 groups as compared to control. Obviously, the growth performance, digestive enzymes activity, immune response, antioxidant statue and antioxidant-related genes expression of R. lagowski fed with the same protein level in BFT system were significantly higher than those in the control group (p < .05). Furthermore, the level of T-SOD in liver and kidney and interferon-γ in liver of BFT33 group were also significantly higher than that of control group (p < .05). Moreover, there was no notable difference of LZM, alkaline phosphatase, complement C3, C4, heat shock proteins 70, heat shock proteins 90, insulin-like growth factor I, glutathione peroxidase, catalase, total antioxidant capacity, malondialdehyde, Ascorbic acid in gill, liver, kidney, gut and serum among the BFT29 groups and those in the control (p > .05). Besides, bioflocs assists in regulating the expression of Nrf2 signalling molecules including Nrf2, Keap1, Maf, HO-1, CAT, CuZn-SOD, GPX and GCLC. We summarized that despite the low protein content in feed, BFT system has the potential to promote growth performance, alleviate copper-induced immunosuppression and oxidative stress of juvenile R. lagowski.

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“…However, antibiotics have the hazard of causing resistance or drug residue to both aquatic animals and humans. Consequently, we urgently need to explore a safe, potential and high‐quality feed additive to perform similar effects (Kong, Ding, et al, 2019; Kong, Gao, et al, 2020; Kong, Kang, et al, 2019; Kong et al, 2020). Over the last research, the use of plants has been widely recognized as a promising natural feed supplement, enhancing the growth and health of aquatic animals in aquaculture (Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The optimum thymol requirement in diets of Channa argus : effects on growth, antioxidant capability, immune response and disease resistance

Kong

Xia

et al. 2021

Aquacult. Nutr.

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In the present study, we aimed to evaluate the effects of dietary thymol on growth, antioxidant status, liver health, immune response and disease resistance of snakehead fish (Channa argus) (initial weight, 11.86 ± 0.15 g). The fish were fed for 56 days with a basal diet supplemented with thymol at 0, 150, 300, 450, 600 and 750 mg/kg, leading to six experimental diets. The final body weight (FBW), weight gain (WG), protein efficiency ratio (PER) and specific growth rate (SGR) were increased as dietary thymol level increased, arriving a summit value at 450 mg/kg dietary thymol level, and then, values of these indices started to decrease when dietary thymol level further rose, and feed conversion ratio (FCR) is the opposite state. Our results showed a significant increase (p < .05) in the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH‐Px) activities in liver, spleen, head kidney and intestine with diets supplemented with 300 and 450 mg/kg thymol compared to the control and significantly decrease (p < .05) in the malondialdehyde (MDA) activity. Results showed that dietary supplementation with 300 and 450 mg/kg thymol significantly increased (p < .05) the serum acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM) activities and immunoglobulin M (IgM), complement 3 (C3), complement 4 (C4) concentrations of C. argus compared to the control, and decreased (p < .05) the serum glutamic‐pyruvic transaminase (ALT) and glutamic‐oxaloacetic transaminase (AST) activities. Moreover, the expression of HSP70, TNF‐α, IL‐1β and IL‐8 were down‐regulated in liver, head kidney and intestine by dietary supplementation with 300 and 450 mg/kg thymol, and the expression of IL‐10 and TGF‐β was up‐regulated. After challenge with Aeromonas veronii, survival rates in all thymol treatment groups were significantly increased (p < .05). Consequently, our results indicate that adequate dietary supplementation thymol can effectively enhance the growth, antioxidant status, immune response and disease resistance of C. argus, and the optimum dietary thymol requirement for maximum WG of C. argus was estimated to be 356 mg/kg.

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Oral immunization with recombinant Lactobacillus casei expressing flaB confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio

Cited by 39 publications

References 27 publications

Surface display system for probiotics and its application in aquaculture

Surface display system for probiotics and its application in aquaculture

Influence of differential protein levels of feed on growth, copper‐induced immune response and oxidative stress of Rhynchocypris lagowski in a biofloc‐based system

The optimum thymol requirement in diets of Channa argus : effects on growth, antioxidant capability, immune response and disease resistance

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