Oral microbiota in human systematic diseases

Peng, Xian; Cheng, Lei; You, Yong; Tang, Cheng-Wei; Ren, Biao; Li, Yuqing; Xu, Xin; Zhou, Xuedong

doi:10.1038/s41368-022-00163-7

Cited by 263 publications

(196 citation statements)

References 188 publications

(185 reference statements)

Supporting

Mentioning

182

Contrasting

Unclassified

Order By: Relevance

“…The findings do not explain the structural characteristics of the oral microbiota. Caries microbial structure and function are far more complex than previously believed [ 33 , 34 ]. Geographic factors affect the dental caries state and the composition of the oral microbiota, and the types of bacteria present in the oral cavity are highly predictive of an individual's place of origin [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments

Guo-yun

Qiao

Shi

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

The characteristics of the oral microbiota may depend on oral health, age, diet, and geography, but the influence of the geographic setting on the oral microbiota has received limited attention. The characteristics of oral microbiota have been reported to differ between urban and rural environments. In order to minimize the influence of genetic background, we recruited 54 volunteers from the same ethnic group, living in urban and rural areas of Gansu Province, China. We collected dental plaque samples and divided them into four groups according to the participant’s area of residence and dental caries status. We sequenced the 16S rRNA of these samples using the Pacific Biosciences sequencing platform and analyzed the correlation between the geographic area and the characteristics of the oral microbiota. Analysis of the alpha and beta diversity revealed that there were significant differences in diversity and composition of dental plaque microflora among the four groups. Cluster analysis revealed that geographic area played an important role in determining the oral microbiota. Network analysis of oral microorganisms showed that geographic differences had major influence on the composition characteristics and internal structure of oral microorganisms. We found that some dominant strains which may play a key role in maintaining oral health, such as Streptococcus oralis, Capnocytophaga sputigena, Porphyromonas catoniae, Corynebacterium matruchotii, Haemophilus parainfluenzae, and Prevotella loescheii, were less affected by the geographic setting. These results provide a deeper understanding of factors influencing the composition of the oral microbiota and could contribute to early diagnosis and effective prevention of dental caries in different settings.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments

Guo-yun

Qiao

Shi

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…The oral microbiota has been extensively studied ( Li et al, 2022 ). This is due to the new insights into the role of the latter in periodontal diseases and systemic diseases including cardiovascular diseases ( Peng et al, 2022 ). Results in HIV infection have been inconsistent and contradictory ( Li et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Mucosal immune cell populations and the bacteriome of adenoids and tonsils from people living with HIV on suppressive antiretroviral therapy

et al. 2022

View full text Add to dashboard Cite

Ear, nose, and throat (ENT) conditions are prevalent in people living with HIV (PLWH) and occur at all strata of CD4 counts and despite antiretroviral therapy (ART). ENT conditions are underreported in PLWH. Also, little is known about the adenotonsillar microbiota and its relation to resident adaptive and innate immune cells. To bridge this gap, we characterized immune cell populations and the bacterial microbiota of two anatomical sites (adenoids, tonsils) and the oral cavity. Adenoids and tonsils were obtained from PLWH (n = 23) and HIV-seronegative individuals (SN, n = 16) after nasal surgery and tonsillectomy and processed for flow cytometry. Nasopharyngeal, oropharyngeal swabs, and oral rinses were collected prior to surgery for 16S sequencing. Wilcoxon rank sum test, principal coordinate analysis, permutational multivariate analysis of variance, and linear discriminant analysis (LEfSe) were used to assess differences between PLWH and SN. Spearman’s correlations were performed to explore interactions between the bacteriome and mucosal immune cells. Of the 39 individuals included, 30 (77%) were men; the median age was 32 years. All PLWH were on ART, with a median CD4 of 723 cells. ENT conditions were classified as inflammatory or obstructive, with no differences observed between PLWH and SN. PLWH had higher frequencies of activated CD4+ and CD8+ T cells, increased T helper (Th)1 and decreased Th2 cells; no differences were observed for B cells and innate immune cells. Alpha diversity was comparable between PLWH and SN at all 3 anatomical sites (adenoids, tonsils, and oral cavity). The impact of HIV infection on the bacterial community structure at each site, as determined by Permutational multivariate analysis of variance, was minor and not significant. Two discriminant genera were identified in adenoids using LEfSe: Staphylococcus for PLWH and Corynebacterium for SN. No discriminant genera were identified in the oropharynx and oral cavity. Niche-specific differences in microbial diversity and communities were observed. PLWH shared less of a core microbiota than SN. In the oropharynx, correlation analysis revealed that Th17 cells were inversely correlated with bacterial richness and diversity, Filifactor, Actinomyces and Treponema; and positively correlated with Streptococcus. Our study contributes toward understanding the role of the adenotonsillar microbiota in the pathophysiology of ENT conditions.

show abstract

“…The oral cavity, which is the entry site into the gastrointestinal tract, harbors diverse microbial communities (Peng et al, 2022), and previous reports support the hypothesis that oral and gastrointestinal mucosae are microbiologically connected (Read et al, 2021;Bao et al, 2022). In addition, the salivary microbiota of periodontitis, which is rich in oral pathobionts, can translocate from the oral cavity to the intestine and disrupt the colonization resistance of gut-resident microbiota (Kitamoto et al, 2020).…”

Section: Introductionmentioning

confidence: 88%

Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats

Wang

Liu

Qian

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The mechanisms underlying the crosstalk between periodontitis and osteoporosis remain unclear. Recently, the gut microbiota has been recognized as a pivotal regulator of bone metabolism, and oral and gut mucosae are microbiologically connected. In this study, we investigated the effects of periodontitis on osteoporosis through the oral-gut axis. The salivary microbiota of patients with periodontitis was collected and then pumped into the intestine of Sprague–Dawley rats via intragastric administration for 2 weeks. An osteoporosis model was established using ovariectomy. Changes in the maxillae and femora were evaluated using microcomputed tomography (micro CT) and HE staining. Intestinal barrier integrity and inflammatory factors were examined using real-time quantitative polymerase chain reaction and immunofluorescence. The gut microbiota was profiled by 16S rRNA gene sequencing. Metabolome profiling of serum was performed using liquid chromatography-mass spectrometry sequencing. Micro CT and HE staining revealed osteoporotic phenotypes in the maxillae and femora of ovariectomized (OVX) rats. Our results confirmed that the salivary microbiota of patients with periodontitis aggravated femoral bone resorption in OVX rats. In addition, intestinal inflammation was exacerbated after periodontitis salivary microbiota gavage in OVX rats. Correlation analysis of microbiota and metabolomics revealed that lipolysis and tryptophan metabolism may be related to the bone loss induced by the salivary microbiota of patients with periodontitis. In conclusion, periodontitis can aggravate long bone loss through the oral-gut axis in OVX rats.

show abstract

Oral microbiota in human systematic diseases

Cited by 263 publications

References 188 publications

Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments

Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments

Mucosal immune cell populations and the bacteriome of adenoids and tonsils from people living with HIV on suppressive antiretroviral therapy

Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats

Contact Info

Product

Resources

About