Oral tissues regeneration using intraoral mesenchymal stem cells

Fagalde, Pascale; Reininger, David

doi:10.4317/jced.56810

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…The fundamental goal of periodontal treatments is to apply reconstruction techniques to restore tissue structure and function. Recent studies have focused on tissue reconstruction or functional recovery after regeneration; of note are the studies on oral mesenchymal stem cells [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells

Choi¹,

Choi²,

Kang

et al. 2021

Biomedicines

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Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis.

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Section: Introductionmentioning

confidence: 99%

Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells

Choi¹,

Choi²,

Kang

et al. 2021

Biomedicines

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“…Numerous in vivo studies on animals and human clinical trials with various types of dental stem cells show a way to encourage novel tissue engineering strategies for therapies of dental diseases[ 109 ]. However, a long road is still ahead, as clinical trials are in their early phases.…”

Section: Clinical Relevance Of Dental Mscsmentioning

confidence: 99%

Dental mesenchymal stromal/stem cells in different microenvironments— implications in regenerative therapy

Okić-Đorđević¹,

Obradović²,

Кукољ³

et al. 2021

WJSC

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Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.

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“…Currently, tissue-engineering-based pulp regeneration therapies mainly include cell homing methods that recruit patients' own endogenous stem cells and odontogenic stem cell transplantation [4] . Because of its high plasticity, good interaction with scaffolds and growth factors, and easy to obtain, odontogenic mesenchymal stem cell has become a research hotspot in the field of pulp regeneration therapy [5] . Scaffolds and growth factors play an important role in pulp regeneration, which can significantly improve the ability of odontogenic stem cells to proliferate and differentiate.…”

Section: Introductionmentioning

confidence: 99%

Research progress of pulp regeneration based on tissue engineering

2024

FMSR

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Pulp regeneration has been a research hotspot in recent years. Functional pulp regeneration can not be achieved without the cooperation of stem cells, growth factors and biological scaffolds. Stem cells, as undifferentiated mesenchymal cells, have the potential of multi-directional differentiation and are often used as seed cells in pulp regeneration, playing a key role. As a kind of fertile soil for supporting cell growth, bio-scaffolds can provide a three-dimensional culture environment for stem cells and promote cell proliferation and differentiation. Growth factors are more as the stimulation of stem cell proliferation and differentiation of protein molecules, playing an important role. In this paper, the application of odontogenic stem cells, scaffolds and growth factors in pulp regeneration was reviewed.

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Oral tissues regeneration using intraoral mesenchymal stem cells

Cited by 5 publications

References 29 publications

Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells

Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells

Dental mesenchymal stromal/stem cells in different microenvironments— implications in regenerative therapy

Research progress of pulp regeneration based on tissue engineering

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