Oral Tolerance to Myelin Basic Protein Induces Regulatory TGF-β-Secreting T Cells in Peyer's Patches of SJL Mice

Santos, Leonilda M.B.; Al‐Sabbagh, Ahmad; Londoño, Ana C.; Weiner, Howard L.

doi:10.1006/cimm.1994.1240

Cited by 149 publications

(82 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PP is an intestinal lymphoid organ and is characterized as an inductive site of mucosal-type immune responses such as Th2 cytokines and transforming growth factor (TGF-b) secretion. 14,15) It has been reported that DC in PP preferentially induced Th2 cytokines, while SPL DC induced Th1 cytokines. 9,10) Our present study shows that antigen presentation by whole PP cells could induce both Th1-and Th2-type cytokine patterns.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the antigen-presentation functions of cells in PP may contribute to a particular type of induction of dose-dependent T cell development in response to the intestinal antigen in vivo. The generation of TGFb-or IL-10-producing regulatory T cells in a lowdose administration, but not high-dose, 14,21) may be an example of these PP functions. It may be possible that these regulatory T cells were primed in the culture and aŠected the Th1 W Th2 diŠerentiation in our experiments, although it is unclear which concentrations in our study correspond to the low or high dose used in the previous in vivo studies.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells

Yoshida

Hachimura

Ishimori

et al. 2002

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells

Yoshida

Hachimura

Ishimori

et al. 2002

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…Capture ELISA for cytokine quantification (13) TGF-ß antibody (polyclonal antibody obtained from R&D Systems, Minneapolis, MN, USA; 1 µg/ml in PBS, pH 7.4) was added to 96-well microtiter plates (Immulon I, Nunc, Roskilde, Denmark). After overnight incubation at 4ºC, the plates were washed three times with ELISA washing buffer (PBS containing 0.05% Tween 20 and 0.001% Thimerosal) and blocked for l h with ELISA diluent (PPB containing 0.05% Tween 20 and l% BSA).…”

Section: Mitogen-induced Blastogenesismentioning

confidence: 99%

Effect of surgical treatment on the cellular immune response of gastric cancer patients

Barbieri

Fujisawa

Yasuda

et al. 2003

Braz J Med Biol Res

View full text Add to dashboard Cite

Patients with gastric cancer have a variety of immunological abnormalities. In the present study the lymphocytes and their subsets were determined in the peripheral blood of patients with gastric cancer (N = 41) both before and after surgical treatment. The percent of helper/ inducer CD4 T cells (43.6 ± 8.9) was not different after tumor resection (43.6 ± 8.2). The percent of the cytotoxic CD8+ T cell population decreased significantly, whether patients were treated surgically (27.2 ± 5.8%, N = 20) or not (27.3 ± 7.3%, N = 20) compared to individuals with inflammatory disease (30.9 ± 7.5%) or to healthy individuals (33.2 ± 7.6%). The CD4/CD8 ratio consequently increased in the group of cancer patients. The peripheral blood lymphocytes of gastric cancer patients showed reduced responsiveness to mitogens. The defective blastogenic response of the lymphocytes was not associated with the production of transforming growth factor beta (TGF-ß) since the patients with cancer had reduced production of TGF-ß1 (269 ± 239 pg/ml, N = 20) in comparison to the normal individuals (884 ± 175 pg/ml, N = 20). These results indicate that the immune response of gastric cancer patients was not significantly modified by surgical treatment when evaluated four weeks after surgery and that the immunosuppression observed was not due to an increase in TGF-ß1 production by peripheral leukocytes. Correspondence

show abstract

“…In rodents, feeding high doses of Ag seems to facilitate anergy/deletion of reactive T cells (6), whereas feeding low doses, probably akin to the physiological situation, induces regulatory cells (7). These regulatory T cells, which can be either CD4 ϩ or CD8 ϩ depending on the system, are primed in the Peyer's patch (PP) 4 of the small intestine and then migrate to the periphery (8,9). When they re-encounter Ag they secrete immunoregulatory cytokines such as TGF-␤, IL-4, or IL-10, which then down-regulate newly initiated or ongoing Th1 cell responses (10).…”

mentioning

confidence: 99%

Human Peyer’s Patch T Cells Are Sensitized to Dietary Antigen and Display a Th Cell Type 1 Cytokine Profile

Nagata

McKenzie

Pender

et al. 2000

The Journal of Immunology

View full text Add to dashboard Cite

Animal studies have demonstrated that feeding Ags induces regulatory (Th2, Th3) cells in Peyer’s patches (PP), which migrate to the periphery and produce immunomodulatory cytokines such as IL-4, IL-10, or TGF-β. In this work we have attempted to extend this paradigm to man by analyzing the response of human PP T cells to in vitro challenge with the common dietary Ag β-lactoglobulin (βlg) of cow’s milk. PP T cells stimulated with βlg showed enhanced proliferation compared with blood T cells from the same patient. Increased expression of CD25 and the Th1-associated chemokine receptor CCR5 was also seen on CD4+ and CD8+ PP T cells, but not blood T cells, stimulated with βlg. By enzyme-linked immunospot assay and RT-PCR, the PP T cell recall response to βlg and casein was dominated by IFN-γ, with negligible IL-4, IL-5, IL-10, or TGF-β. To help explain the PP T cell response to βlg, we examined IL-12 expression. Both IL-12p40 and -p35 transcripts were abundantly expressed in PP, but not in adjacent normal ileal mucosa. Immunoreactive IL-12p40-containing cells were present below the PP dome epithelium. Furthermore, in culture, PP, but not paired PBMC, spontaneously released IL-12p70. These results suggest that the human response to oral Ags in the gut may be different from that in rodents.

show abstract

Oral Tolerance to Myelin Basic Protein Induces Regulatory TGF-β-Secreting T Cells in Peyer's Patches of SJL Mice

Cited by 149 publications

References 0 publications

Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells

Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells

Effect of surgical treatment on the cellular immune response of gastric cancer patients

Human Peyer’s Patch T Cells Are Sensitized to Dietary Antigen and Display a Th Cell Type 1 Cytokine Profile

Contact Info

Product

Resources

About