Orchestrating the cell cycle in yeast: sequential localization of key mitotic regulators at the spindle pole and the bud neck

Cid, Vı Ctor J; Jiménez, Javier; Molina, Marı A; Sánchez, Miguel; Nombela, César; Thorner, Jeremy

doi:10.1099/00221287-148-9-2647

Cited by 30 publications

(37 citation statements)

References 130 publications

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“…Andrews, unpublished), but the in vivo significance of these phosphorylation events is still under study. Furthermore, as mentioned earlier, there are additional protein kinases (and phosphoprotein phosphatases) that localize to the bud neck [14,27]. Clearly, we are just beginning to understand how phosphorylation of septins (and septin-associated proteins) influences the different facets of septin organization, dynamics and function.…”

Section: Phosphorylation Exerts Temporal Control On Septin-collar Assmentioning

confidence: 95%

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Some assembly required: yeast septins provide the instruction manual

Versele

Thorner

2005

Trends in Cell Biology

201

199

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Septins are a family of conserved proteins that form hetero-oligomeric complexes that assemble into filaments. The filaments can be organized into linear arrays, coils, rings and gauzes. They serve as membrane-associated scaffolds and as barriers to demarcate local compartments, especially for the establishment of the septation site for cytokinesis. Studies in budding and fission yeast have revealed many of the protein-protein interactions that govern the formation of multi-septin complexes. GTP binding and phosphorylation direct the polymerization of filaments that is required for septin-collar assembly in budding yeast, whereas a homolog of anillin instructs timely formation of the ring of septin filaments at the medial cortex in fission yeast. These insights should aid understanding of the organization and function of the diverse septin structures in animal cells.

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Section: Phosphorylation Exerts Temporal Control On Septin-collar Assmentioning

confidence: 95%

“…Cdc5, Cdc15, Dbf2, Elm1, Gin4, Hsl1, Kcc4 and Ssk2), other enzymes (e.g. chitin synthases) and components of the bud-site-selection machinery (for an exhaustive catalog, see [14,27]). In many instances, the binding is direct [28].…”

Section: Septin Functionsmentioning

confidence: 99%

Some assembly required: yeast septins provide the instruction manual

Versele

Thorner

2005

Trends in Cell Biology

201

199

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“…Cdc5p is a yeast orthologue of polo-like kinase, a conserved protein kinase family found in many eukaryotic organisms from yeast to mammals. It plays multiple key roles in the coordination of mitosis (30,45). CDC5 was identified as a multicopy suppressor of various mutants related to progression of the M phase, such as CDC15, CDC20, DBF2, and TEM1 (46,47).…”

Section: Roles Of Multicopy Suppressors In Gpi7mentioning

confidence: 99%

GPI7 Involved in Glycosylphosphatidylinositol Biosynthesis Is Essential for Yeast Cell Separation

Fujita

Yoko-o

Okamoto

et al. 2004

Journal of Biological Chemistry

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GPI7 is involved in adding ethanolaminephosphate to the second mannose in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae. We isolated gpi7 mutants, which have defects in cell separation and a daughter cell-specific growth defect at the non-permissive temperature. WSC1, RHO2, ROM2, GFA1, and CDC5 genes were isolated as multicopy suppressors of gpi7-2 mutant. Multicopy suppressors could suppress the growth defect of gpi7 mutants but not the cell separation defect. Loss of function mutations of genes involved in the Cbk1p-Ace2p pathway, which activates the expression of daughter-specific genes for cell separation after cytokinesis, bypassed the temperaturesensitive growth defect of gpi7 mutants. Furthermore, deletion of EGT2, one of the genes controlled by Ace2p and encoding a GPI-anchored protein required for cell separation, ameliorated the temperature sensitivity of the gpi7 mutant. In this mutant, Egt2p was displaced from the septal region to the cell cortex, indicating that GPI7 plays an important role in cell separation via the GPI-based modification of daughter-specific proteins in S. cerevisiae.

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“…Therefore, we will summarize below three aspects of septin regulation that have been relatively well studied: regulation by the Rho-family GTPase Cdc42, phosphorylation, and GTP binding. Some of the other proteins that bind septins and may regulate their function include Bni5, Iqg1, and Hof1 (18,30,40,64).…”

Section: Regulation Of Septin Ring Assembly and Disassembly During Thmentioning

confidence: 99%

Septin Function in Yeast Model Systems and Pathogenic Fungi

Douglas¹,

Álvarez

McCreary³

et al. 2005

Eukaryot Cell

106

101

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The septins were first discovered in the budding yeast Saccharomyces cerevisiae and were named for their role in cytokinesis and septum formation (69). Septins are now known to be highly conserved in fungi and animals, although absent in plants and many protozoans (e.g., Plasmodium fasciculatum and Dictyostelium discoideum). The septin proteins are characterized by presence of a distinct type of GTPase domain and by their ability to form filaments. The S. cerevisiae septin proteins form a series of 10-nm filaments that assemble into a ring on the inner surface of the plasma membrane at the bud neck. Septin rings are thought to function as a scaffold to recruit proteins to the bud neck and to act as a boundary domain to restrict diffusion during budding and cytokinesis (30,39,66,68). However, septins are now implicated in a broad range of dynamic membrane events. In S. cerevisiae, septins have been found to also play a role in conjugation and sporulation. Moreover, analyses of septin function in other organisms, including fungi that undergo different patterns of growth and differentiation, are revealing new aspects of septin function. Therefore, we will provide an overview of the current understanding of the relatively well-studied roles of septins during S. cerevisiae budding and then use this as a context to review studies of septin function during other developmental pathways in S. cerevisiae and other fungi. The other fungi will include the model fission yeast Schizosaccharomyces pombe and two opportunistic fungal pathogens: the multimorphic Candida albicans and the filamentous fungus Aspergillus nidulans.

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Orchestrating the cell cycle in yeast: sequential localization of key mitotic regulators at the spindle pole and the bud neck

Cited by 30 publications

References 130 publications

Some assembly required: yeast septins provide the instruction manual

Some assembly required: yeast septins provide the instruction manual

GPI7 Involved in Glycosylphosphatidylinositol Biosynthesis Is Essential for Yeast Cell Separation

Septin Function in Yeast Model Systems and Pathogenic Fungi

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