Orchid Seed Ultrastructure: Ecological and Taxonomic Implications with Reference to Epidendroideae (Orchidaceae)

Ramudu, J.; Khasim, S. M.; Ramesh, G.

doi:10.1007/978-981-32-9456-1_14

Cited by 2 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the shape (fusiform) and pigmentation (whitish-yellow) in C. crispa seeds have also been reported in other epiphytic orchids, including the Cattleya genus (Diantina et al, 2020). Ramudu et al (2020) indicate that fusiform seeds appear to be the basic form from which all other seed shapes evolved in this family.…”

Section: Discussionmentioning

confidence: 67%

Morpho-anatomy of in vitro germination and cryopreservation of the orchid Cattleya crispa (Orchidaceae)

Vargas Andriolli,

Corredor-Prado,

Pescador

et al. 2023

Rev. Biol. Trop.

View full text Add to dashboard Cite

Introduction: Cattleya crispa is an ornamental epiphytic orchid with geographic distribution restricted to the Brazilian Atlantic Forest. Due to predatory extractivism and human-induced habitat loss, this species appears on the Red List of Brazilian Flora. Objective: To characterize morpho-anatomical aspects regarding germination and post-seminal development from C. crispa seeds; as well as studying the effect of cryopreservation on these seeds. Methods: We used light microscopy and electron microscopy to describe the microstructure of a 100 ripe seeds. We evaluated seed viability, seed germination, survival rate and protocorm weight in cryopreserved and non-cryopreserved material, with four replicas per treatment using 20 mg of plant material. Results: The seeds are fusiform, whitish yellow with a length from 700 to 900 µm and a water content of 5 %. Germination began seven days after sowing, the formation of the globular protocorm at 30 days and the formation of the seedling occurred 150 days. The persistent seed coat can compress the protocorm and cause it to collapse. The cryopreserved seeds presented 87.15 % viability, 78.32 % germination, 8.48 % survival and protocorms with 104.27 mg five months after sowing. Data wasn’t different to non-cryopreserved seeds. Conclusions: The cryocapability of the seeds shows that cryopreservation can be used for long-term conservation. The results of this work contribute to the overall biology of C. crispa and to the propagation and storage of genetic material for conservation purposes. Objective: To characterize morpho-anatomical aspects regarding germination and initial development from C. crispa seeds; as well as studying the effect of cryopreservation on these seeds. Methods: Mature capsules of C. crispa were collected from the ex-situ conservation collection of the Orquidário Frederico Carlos Hoehne - Institute of Botany (São Paulo-Brazil). We used light microscopy, scanning electron microscopy and transmission electron microscopy. Seed viability (%), seed germination (%), survival rate (%) and protocorms weight (mg) were evaluated. Results: Our analyses showed that the globular embryo has a homogeneously early germination that occurs by the 7th day. The germinating protocorm shows bipolarity, with shoot apex and basal region. The embryo is composed mostly of lipid and protein bodies and contains a few small grains of starch. Scanning electron microscopy images revealed a persistent seed coat that does not influence the onset of germination, however, can compress the protocorm and lead to its collapse. The initial water content of the seeds was 5 %. Seeds submitted to cryopreservation resulted in a high germination rate (81.14 %). However, the survival rate of non-cryopreserved (8.48 %) and cryopreserved (11.12 %) protocorms, evaluated five months after sowing, was reduced. Conclusions: The cryocapability of the seeds shows that cryopreservation can be used for long-term conservation. The results of this work contribute to the overall biology of C. crispa and to the propagation and storage of genetic material for conservation purposes.

show abstract

Section: Discussionmentioning

confidence: 67%

Morpho-anatomy of in vitro germination and cryopreservation of the orchid Cattleya crispa (Orchidaceae)

Vargas Andriolli,

Corredor-Prado,

Pescador

et al. 2023

Rev. Biol. Trop.

View full text Add to dashboard Cite

show abstract

“…This species is uncommon and usually found only in isolated stands, growing on tree trunks of the lower and middle layers of the forest (O'Byrne 1994). The reason for this condition is that they have a very specific environment (habitat and substrate) and mycorrhiza requirements for seed germination (Arditti and Ghani 2000;Tongbram et al 2012;Ramudu et al 2020). Zhang et al (2018) stated that orchids display various levels of specificity to their fungal hosts, then it can be seen clearly that the environment with low fungal diversity would restrict the orchids to only certain habitats where these specific fungi appear.…”

Section: Seed Air Space In Relation To Dispersal Area Of the Speciesmentioning

confidence: 99%

“…Zhang et al (2018) stated that orchids display various levels of specificity to their fungal hosts, then it can be seen clearly that the environment with low fungal diversity would restrict the orchids to only certain habitats where these specific fungi appear. There would be heavy mortality at any stage of seedling development and very few seed seedlings will grow successfully and give a rise to mature plants (Ramudu et al 2020).…”

Section: Seed Air Space In Relation To Dispersal Area Of the Speciesmentioning

confidence: 99%

See 1 more Smart Citation

A seed morphometry study of selected species of Bulbophyllum and Dendrobium (Orchidaceae) in relation to their dispersals

2021

View full text Add to dashboard Cite

Abstract. Aprilianti P, Handini E, Puspitaningtyas DM. 2021. A seed morphometry study of selected species of Bulbophyllum and Dendrobium (Orchidaceae) in relation to their dispersals. Biodiversitas 22: 5564-5571. Bulbophyllum and Dendrobium are the two largest genera of Orchidaceae. The seed morphometry studies of Bulbophyllum and Dendrobium, mainly from Southeast Asia are still poorly reported. The research aimed to characterize seeds micromorphometry of selected species of Bulbophyllum and Dendrobium, namely Bulbophyllum graveolens, B. macranthum, B. phalaenopsis, Dendrobium bicaudatum, and D. bifalce in relation to their dispersal mechanisms. Twenty seeds of each species were studied using light and scanning electron microscope (SEM). Qualitative characters, such as seed shape, testa cell shape, wall structure, and its orientation and quantitative data, such as seed and embryo were analyzed. The results showed that the shape of the seeds of all the species was fusiform. The testa cell walls were smooth and covered by waxes. The anticlinal walls were raised and thickened. Bulbophyllum and Dendrobium can be differentiated in the orientation of testa cells, which is spirally oriented in Bulbophyllum and parallel to the long axis in the Dendrobium. There are no large variations in seed and embryo volume as well as seed air space (SAS) in different taxa. The SAS value can be used to predict the dispersal mechanism and distribution area of the orchid species. Unfortunately, the SAS values are not always positively correlated with the distribution area and other factors inhibit the spread of the species.

show abstract

Orchid Seed Ultrastructure: Ecological and Taxonomic Implications with Reference to Epidendroideae (Orchidaceae)

Cited by 2 publications

References 31 publications

Morpho-anatomy of in vitro germination and cryopreservation of the orchid Cattleya crispa (Orchidaceae)

Morpho-anatomy of in vitro germination and cryopreservation of the orchid Cattleya crispa (Orchidaceae)

A seed morphometry study of selected species of Bulbophyllum and Dendrobium (Orchidaceae) in relation to their dispersals

Contact Info

Product

Resources

About