Ordered honeycomb biocompatible polymer films via a one-step solution-immersion phase separation used as a scaffold for cell cultures

Bui, Van‐Tien; Thủy, Lê Thị Thanh; Tran, Quoc Chinh; Nguyen, Van Thang; Dao, Van‐Duong; Choi, Ji Soo; Choi, Ho‐Suk

doi:10.1016/j.cej.2017.03.086

Cited by 38 publications

(31 citation statements)

References 20 publications

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“…In ref. [ 9 ], Bui et al reported a slighty opposite trend. This discrepancy can be explained by the fact that, although the conditions of the experiment were very similar, they differ in the use of the substrate.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the enrichment of methanol in the solution through solvent evaporation is favorable for absorbing water vapor from the ambient environment. Secondly, the high solubility with water-immiscible solvents, such as chloroform, was reported, and last but not least, methanol accumulation was reported to induce the transport of polymers to the surface of nonsolvent droplets [ 9 ]. It was also reported that the pore sizes of the honeycomb films can be further tuned by modulating the ambient humidity and polymer concentration [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…Secondly, the high solubility with water-immiscible solvents, such as chloroform, was reported, and last but not least, methanol accumulation was reported to induce the transport of polymers to the surface of nonsolvent droplets [ 9 ]. It was also reported that the pore sizes of the honeycomb films can be further tuned by modulating the ambient humidity and polymer concentration [ 9 ]. One of the results presented in [ 9 ] was that the pore sizes slightly decreased with an increasing concentration of the biopolymer in the solution.…”

Section: Resultsmentioning

confidence: 99%

“…It was also reported that the pore sizes of the honeycomb films can be further tuned by modulating the ambient humidity and polymer concentration [ 9 ]. One of the results presented in [ 9 ] was that the pore sizes slightly decreased with an increasing concentration of the biopolymer in the solution. Our findings regarding the influence of the amount of methanol are in good agreement with Bui [ 9 ], and our preliminary testing of an increasing humidity also confirmed the results reported in the experiment of [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…Some techniques are more demanding stepwise (e.g., lithography) [ 7 ], and some take place under more demanding conditions (e.g., a breath figure, which requires a humid environment) [ 8 ]. Bui et al showed how to solve these limitations and designed a uniform HCP-like film via improved phase separation, which is a simple and inexpensive method [ 9 ]. The improvement is based on the presence of methanol in a binary mixture of organic solvents.…”

Section: Introductionmentioning

confidence: 99%

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PLLA Honeycomb-Like Pattern on Fluorinated Ethylene Propylene as a Substrate for Fibroblast Growth

et al. 2020

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In this study, we present the surface patterning of a biopolymer poly(l-lactide) (PLLA) for fibroblast growth enhancement. The patterning is based on a self-organized pore arrangement directly fabricated from a ternary system of a solvent-nonsolvent biopolymer. We successfully created a porous honeycomb-like pattern (HCP) on a thermally resistant polymer—fluorinated ethylene propylene (FEP). An important preparation step for HCP is activation of the substrate in Ar plasma discharge. The polymer activation leads to changes in the surface chemistry, which corresponds to an increase in the substrate surface wettability. The aim of this study was to evaluate the influence of the PLLA concentration in solution on the surface morphology, roughness, wettability, and chemistry, and subsequently, also on fibroblast proliferation. We confirmed that the amount of PLLA in solution significantly affects the material surface properties. The pore size of the prepared layers, the surface wettability, and the surface oxygen content increased with an increasing amount of biopolymer in the coating solution. The optimal amount was 1 g of PLLA, which resulted in the highest number of cells after 6 days from seeding; however, all three biopolymer concentrations exhibited significantly better results compared to pristine FEP. The cytocompatibility tests showed that the HCP promoted the attachment of cell filopodia to the underlying substrate and, thus, significantly improved the cell–material interactions. We prepared a honeycomb biodegradable support for enhanced cell growth, so the surface properties of perfluoroethylenepropylene were significantly enhanced.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

PLLA Honeycomb-Like Pattern on Fluorinated Ethylene Propylene as a Substrate for Fibroblast Growth

et al. 2020

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Water transferable, customizable highly ordered honeycomb film from polystyrene foam waste for complex surface patterning in confined space

Le,

Chau,

Van Le

et al. 2024

J of Applied Polymer Sci

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The disposal of plastic foam, mostly composed of polystyrene, poses significant environmental challenges due to its high popularity, slow degradation, and low cost. To address this problem, recycling polystyrene foam waste (PF) has emerged as a promising solution to reduce plastic pollution. This paper presents a novel approach to mass‐produce highly ordered, porous honeycomb‐patterned film (hc‐film) using wasted PF as the raw material. The hc‐film is produced using an improved phase separation (IPS) method that utilizes methanol as a suitable pore inducer and template droplet stabilizer. Methanol provides the hc‐film with customizable features such as pore ordering, size, and separation. The freestanding hc‐film, achieved by adopting a water‐soluble polystyrene sulfonate as a scarified layer, can be transferred and utilized as a flexible mold to pattern various solid substrates with complicated surface morphologies using the pre‐impregnated technique. This study demonstrates the potential of this cost‐effective and efficient approach for various applications, such as super/anti‐wetting surfaces, microelectronics, optical devices, sensors, and nanogenerators.

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Effect of Pore Size of Honeycomb‐Patterned Polymer Film on Spontaneous Formation of 2D Micronetworks by Coculture of Human Umbilical Vein Endothelial Cells and Mesenchymal Stem Cells

Oku

Ohno

Miyamoto

et al. 2021

Macromolecular Bioscience

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The geometrical control of micronetwork structures (μNSs) formed by endothelial cells is an important topic in tissue engineering, cell‐based assays, and fundamental biological studies. In this study, μNSs are formed using human umbilical vein endothelial cells (HUVECs) by the coculture of HUVECs and human mesenchymal stem cells (MSCs) confined in a honeycomb‐patterned poly‐l‐lactic acid film (honeycomb film (HCF)), which is a novel cell culture scaffold. The HCF is produced using the breath figure method, which uses condensed water droplets as pore templates. The confinement of the HUVECs and MSCs in the HCF along with the application of centrifugal force results in μNS formation when the pore size is more than 20 μm. Furthermore, μNS development is geometrically restricted by the hexagonally packed and connected pores in the horizontal direction of the HCF. Network density is also controlled by changing the seeding density of the HUVECs and MSCs. The threshold pore size indicates that μNSs can be formed spontaneously by using an HCF with a perfectly uniform porous structure. This result provides an important design guideline for the structure of porous cell culture scaffolds by applying a blood vessel model in vitro.

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Ordered honeycomb biocompatible polymer films via a one-step solution-immersion phase separation used as a scaffold for cell cultures

Cited by 38 publications

References 20 publications

PLLA Honeycomb-Like Pattern on Fluorinated Ethylene Propylene as a Substrate for Fibroblast Growth

PLLA Honeycomb-Like Pattern on Fluorinated Ethylene Propylene as a Substrate for Fibroblast Growth

Water transferable, customizable highly ordered honeycomb film from polystyrene foam waste for complex surface patterning in confined space

Effect of Pore Size of Honeycomb‐Patterned Polymer Film on Spontaneous Formation of 2D Micronetworks by Coculture of Human Umbilical Vein Endothelial Cells and Mesenchymal Stem Cells

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