Orexin-A expression in human peripheral tissues

Nakabayashi, Masao; Suzuki, Takashi; Takahashi, Kazuhiro; Totsune, Kazuhito; Muramatsu, Yasunari; Kaneko, Chika; Date, Fumiko; Takeyama, Junji; Darnel, Andrew D.; Moriya, Takuya; Sasano, Hironobu

doi:10.1016/s0303-7207(03)00206-5

Cited by 152 publications

(112 citation statements)

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“…The role of those peptides in uterine/placental physiology remains unknown. To our best knowledge, the presence of the orexin system components in human and feline placenta and uteri has been demonstrated by very few studies (Nakabayashi et al, 2003;Dall'Aglio et al, 2012;Dehan et al, 2013), including our study of the porcine uterus (Nitkiewicz et al, 2012). The involvement of orexins in the control of endometrial epithelial cell proliferation through type 2 receptors has been suggested in only one study (Dehan et al, 2013).…”

Section: Discussionmentioning

confidence: 85%

“…Local orexin production (Nitkiewicz et al, 2014) and the presence of specific receptors in porcine and rat ovaries (Silveyra et al, 2007a;Nitkiewicz et al, 2010) indicate that orexins could also exert direct endocrine and auto/paracrine effects inside glands. OXA was found in human and feline placenta, OX1R protein was identified in feline placenta (in syncytiotrophoblast and decidual cells derived from the endometrium), whereas the presence of OX2R protein was noted only in cells forming the endometrial glands (Nakabayashi et al, 2003;Dall'Aglio et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Expression of the orexin system in the porcine uterus, conceptus and trophoblast during early pregnancy

Smolińska

Kieżun

Szeszko

et al. 2015

Animal

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Orexin A and B are hypothalamic peptides derived from the prepro-orexin (PPO) precursor. Orexins stimulate food intake and arousal. Those peptides bind and activate two G protein-coupled receptors: orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). Numerous authors have suggested that orexins play an important role in the regulation of the reproductive functions. The objective of the present study was to analyse the presence of and changes in the gene and protein expression pattern of the orexin system in the porcine uterus, conceptus and trophoblast (chorioallantois) during early pregnancy. In the endometrium, the highest PPO and OX1R gene expression was detected on days 15 to 16 of gestation. The OX2R mRNA content in the endometrium was higher on days 10 to 11 and 15 to 16 than on days 12 to 13 and 27 to 28. In the trophoblasts, PPO gene expression was higher on days 30 to 32 than on days 27 to 28. The highest PPO protein content in the endometrium was noted on days 12 to 13. The highest OX1R protein content in the endometrium was detected on days 10 to 11, whereas OX2R protein on days 15 to 16. In the trophoblasts, PPO and OX1R protein levels were more pronounced on days 27 to 28 than on days 30 to 32, but OX2R expression was higher on days 30 to 32. The expression of PPO, OX1R and OX2R was different in the conceptuses and trophoblasts during early pregnancy. Local orexin production and the presence of the specific orexin receptors suggest that the orexin system may participate in the control of porcine reproductive functions by exerting endocrine and auto/paracrine effects on the uterus, conceptuses and trophoblasts during early pregnancy. This study provides the first evidence for the presence of orexins and their receptors in the uteri, conceptuses and trophoblasts in pigs during early pregnancy. The local orexin system is dependent on the stage of pregnancy.Keywords: orexin A and B, orexin receptors 1 and 2, uterus, pregnancy, pig ImplicationsThere is a growing body of evidence to implicate the existence of a common endocrine system that controls metabolism and the reproductive system. Orexin A and orexin B, the hormones that control energy homoeostasis, may be a part of the above system. This paper provides the pioneering information about the presence of orexins and orexin receptors in the porcine uterus, conceptus and trophoblast during early pregnancy, the crucial period for reproductive success. The expression levels of orexins and their receptors were found to be correlated with the stages of early pregnancy. A sound knowledge of mechanisms that control energy homoeostasis and reproduction is needed in animal breeding practice.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Expression of the orexin system in the porcine uterus, conceptus and trophoblast during early pregnancy

Smolińska

Kieżun

Szeszko

et al. 2015

Animal

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“…To our knowledge, Nakabayashi et al [28] were the first to report the absence of OXA-like immunoreactivity (LI) in the human prostate. The expression of genes coding for ppOX in the urethroprostatic complex of cattle was demonstrated by PCR and Western blot, and IHC revealed OXA-LI substances in the exocrine epithelium of the gland [15].…”

Section: Discussionmentioning

confidence: 99%

Lack of expression of preproorexin and orexin receptors genes in human normal and prostate cancer cell lines

Szyszka

Paschke

Tyczewska

et al. 2016

Folia Histochem Cytobiol.

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Introduction. Studies on expression of orexins (OXs) and their receptors in human prostate gland and human prostatic cell lines are scanty and their results contradictory. Regarding this, we carefully reinvestigated this problem on human prostatic cell lines. Material and methods. Expression of preproorexin (ppOX) (6 primer pairs), and orexin receptors 1 and 2 (OXR1, OXR2) (4 and 2 primer pairs, respectively) was assessed by conventional PCR and QPCR in human normal (PrEC, PrSc, PrSmC) and prostate carcinoma (Du145, LNCaP, and PC3) cell lines. We designed intron spanning primers and also we applied primers from earlier publications and commercially available ones. Results. With the designed primer pairs, in all studied cell lines we failed to demonstrate expression of ppOX, OXR1 and OXR2 genes at the mRNA level, while reaction products were observed in control tissues (human placenta and adrenals). Primers applied in earlier studies did not form amplification products specific for preproorexin or orexin 1 receptor. Some commercially available primers for orexin receptor 1 produced false positive results. Conclusions. We found no evidence for the presence of preproorexin-orexin receptors system genes' mRNAs in human prostate cell lines. The reported premises for these genes' expression in prostate and prostatic cell lines may have arisen either from the presence of non-prostate cells included in the samples or from faulty PCR settings.

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“…Orexin Expression. Low pp-orexin mRNA expression has been detected by RT-PCR in human adrenal glands (Nakabayashi et al, 2003), and the presence of pp-orexin and orexin-A proteins has been demonstrated by Western blotting in both adult and fetal (2nd trimester) human glands Randeva et al, 2001), but more recent studies did not confirm these findings (Spinazzi et al, 2005b). No orexin expression was found in the rat adrenal glands (Lopez et al, 1999Karteris et al, 2005).…”

Section: A Expression Of Orexins and Their Receptorsmentioning

confidence: 99%