Organic fluorescent probes for detecting mitochondrial membrane potential

Li, Xuechen; Zhao, Yuping; Yin, Junling; Lin, Weiying

doi:10.1016/j.ccr.2020.213419

Cited by 78 publications

(40 citation statements)

References 69 publications

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“…Several upregulated genes have been shown to restrain basal mitochondrial respiratory capacity with respective mechanisms other than mitophagy, including BNIP3 (Zhang et al, 2009;Rikka et al, 2011;Liu et al, 2017b), BNIP3L (Mughal et al, 2018;da Silva Rosa et al, 2020), HMOX1 (Zukor et al, 2009;Meyer et al, 2018), PDK4 (Oh et al, 2017;Ma et al, 2020), and MIR210 (microRNA-210) (Chan et al, 2009;Nakada et al, 2020; Table 1). The mitochondrial membrane potential ( ψm) normally remains stable for the respiratory chain to generate ATP and thus can be used as a fundamental indicator of mitochondrial function (Zorova et al, 2018;Li et al, 2020). We found that ψm was reduced after 24 h of FG-4592 treatment, which was further exacerbated after 48 h (Figures 4G,H), suggesting that mitochondrial function was compromised by activation of HIF and downstream factors before substantial mitophagy induction.…”

Section: Hif1α Activation Decreased Fa β-Oxidationmentioning

confidence: 91%

Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1α in Renal Tubules

Duan

Xing

et al. 2021

Front. Cell Dev. Biol.

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Lipid metabolism plays a basic role in renal physiology, especially in tubules. Hypoxia and hypoxia-induced factor (HIF) activation are common in renal diseases; however, the relationship between HIF and tubular lipid metabolism is poorly understood. Using prolyl hydroxylase inhibitor roxadustat (FG-4592), we verified and further explored the relationship between sustained HIF1α activation and lipid accumulation in cultured tubular cells. A transcriptome and chromatin immunoprecipitation sequencing analysis revealed that HIF1α directly regulates the expression of a number of genes possibly affecting lipid metabolism, including those associated with mitochondrial function. HIF1α activation suppressed fatty acid (FA) mobilization from lipid droplets (LDs) and extracellular FA uptake. Moreover, HIF1α decreased FA oxidation and ATP production. A lipidomics analysis showed that FG-4592 caused strong triglyceride (TG) accumulation and increased some types of phospholipids with polyunsaturated fatty acyl (PUFA) chains, as well as several proinflammatory lipids. Nevertheless, the overall FA level was maintained. Thus, our study indicated that HIF1α reduced the FA supply and utilization and reconstructed the composition of lipids in tubules, which is likely a part of hypoxic adaptation but could also be involved in pathological processes in the kidney.

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Section: Hif1α Activation Decreased Fa β-Oxidationmentioning

confidence: 91%

Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1α in Renal Tubules

Duan

Xing

et al. 2021

Front. Cell Dev. Biol.

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“…Three main families of fluorescent probes have been defined according to their detection method [42]. Monochromatic and ratiometric fluorescence probes are most popular and include, but are not limited to, the commercially available probes Rhodamine 123 (Rhod 123) [229], 3,3 -dihexyloxacarbocyanine iodide [DiOC 6 (3)] [230], tetramethylrhodamine methyl ester perchlorate (TMRM) or tetramethylrhodamine ethyl ester perchlorate (TMRE) [40,231], the 5,5 ,6,6 -tetrachloro-1,1 ,3,3 -tetraethylbenzimidazolcarbocyanine iodide (JC-1) [232] and the mitochondrial membrane potential indicator (mMPI) [41].…”

Section: Mitochondrial Membrane Potential Evaluation: Fluorescent Probe-based Assaysmentioning

confidence: 99%

“…Therefore, the selection of a specific dye and the interpretation of the results should be carefully performed. Linked to this, the use of fluorescent probes should be accompanied by appropriate pharmacologic controls as well as complementary dyes, which allows to increase the robustness of the results [39][40][41][42][43]. The 2 ,7 -dichlorodihydrofluorescein diacetate (DCFH 2 -DA) assay is one of the most widely used fluorescent probe-based methods to evaluate intracellular reactive oxygen species (ROS) levels.…”

Section: Mitochondrial Membrane Potential Evaluation: Fluorescent Probe-based Assaysmentioning

confidence: 99%

In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach

Tabernilla

Rodrigues

Pieters

et al. 2021

IJMS

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The liver is among the most frequently targeted organs by noxious chemicals of diverse nature. Liver toxicity testing using laboratory animals not only raises serious ethical questions, but is also rather poorly predictive of human safety towards chemicals. Increasing attention is, therefore, being paid to the development of non-animal and human-based testing schemes, which rely to a great extent on in vitro methodology. The present paper proposes a rationalized tiered in vitro testing strategy to detect liver toxicity triggered by chemicals, in which the first tier is focused on assessing general cytotoxicity, while the second tier is aimed at identifying liver-specific toxicity as such. A state-of-the-art overview is provided of the most commonly used in vitro assays that can be used in both tiers. Advantages and disadvantages of each assay as well as overall practical considerations are discussed.

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“…During the process of energy production in mitochondria, the concentration of various ions including protons is distributed on inner and outer membranes of mitochondria, which results in mitochondrial membrane potential [ 13 , 41 ]. The integrity of MMP is highly related to mitochondrial functions and there is a lot of evidence that the abnormality of MMP is associated with human diseases such as Parkinson’s disease, Alzheimer’s disease, and cancer [ 7 , 9 , 11 , 13 , 42 , 43 ]. Therefore, fluorescent probes for detecting MMP provide a great research tool for mitochondrial biology.…”

Section: Fluorescent Chemical-based Mitochondria Probesmentioning

confidence: 99%

“…With a high sensitivity and signal-to-noise ratio, simple operation, practicality, good selectivity, and the capability for real-time detection, fluorescence has been utilized as a promising technique. From monochromatic fluorescent probes to ratiometric, multi-photon probes, and target-switchable fluorescent probes, a variety of fluorescent chemical probes has been developed [ 13 ]. Due to the high extinction coefficients, quantum yields, and modularity, the engineering of multicolored fluorescent proteins has enabled the development of various protein sensors [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Materials for Monitoring Mitochondrial Biology

et al. 2021

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Mitochondria play important roles in diverse cellular processes such as energy production, cellular metabolism, and apoptosis to promote cell death. To investigate mitochondria-associated biological processes such as structure, dynamics, morphological change, metabolism, and mitophagy, there exists a continuous demand for visualizing and monitoring techniques elucidating mitochondrial biology and disease-relevancy. Due to the advantages of high sensitivity and practicality, fluorescence phenomena have been most widely used as scientific techniques for the visualization of biological phenomena and systems. In this review, we briefly overview the different types of fluorescent materials such as chemical probes, peptide- or protein-based probes, and nanomaterials for monitoring mitochondrial biology.

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Organic fluorescent probes for detecting mitochondrial membrane potential

Cited by 78 publications

References 69 publications

Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1α in Renal Tubules

Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1α in Renal Tubules

In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach

Fluorescent Materials for Monitoring Mitochondrial Biology

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