Organic Matter Decomposition in River Ecosystems: the Role of Microbial Interactions Regulated by Environmental Factors

Abstract

Microbes are the critical contributors to the organic matter decomposition (OMD) in river ecosystems. However, the role of microbial interactions on the OMD in river ecosystems and the regulation of environmental factors to the microbial interactions were not considered previously thus tacked in this study. Cotton strip (CS) as a substitute for organic matter was introduced to Luanhe River Basin in China. The results indicated that CS selectively enriched bacterial and fungal groups related to cellulose decomposition, leading to the cotton strip decomposition (CSD). In these groups, bacterial phyla Proteobacteria, fungal phyla Rozellomycota and Ascomycota were the dominant groups associated with the CSD. Bacteria and fungi on CS cooperatively formed a co-occurrence network to achieve the CSD. In the network, the key modules 2 and 4, mainly composed of phyla Proteobacteria and Ascomycota, directly promoted the CSD. Keystone taxa maintained the stability of microbial network structure and function, and regulated microbial groups associated with CSD in the key modules, rather than directly decomposing the CS. Notably, this study profoundly revealed that water temperature and total nitrogen (TN) regulated the keystone taxa and key modules in microbial interactions and then promoted the OMD. The two key modules 2 and 4 were significantly correlated with water temperature and TN in water, and two keystone taxa (bacterial genera Emticicia and Flavihumibacter) were significantly associated with TN. The research findings help us to understand the microbial mechanism of the OMD in rivers, which provides valuable insights into improving effective management strategies for river ecosystems.