This research was carried out at the experimental Farm, Faculty of Agriculture, Al-Azhar University, Assiut, during the two successive seasons of 2018 and 2019 aiming to study the influence of filter mud as organic manure stimulant substances namely (amino acids {tryptophan + methionine}, active yeast and seaweed extract), as well as, their Interactions on plant growth traits and chemical constituents of Khaya (Khaya senegalensis) seedlings. The soil of Khaya seedlings was supplied with filter mud at 0, 18, 36 and 54 g/bag and the seedlings were sprayed with stimulant substances, whereas the treatments of these materials were: control, amino acids at 100 ppm, active yeast at 5 g/l, seaweed extract at 3 ml/l, amino acids + active yeast, seaweed + active yeast, amino acids + seaweed extract and amino acids + active yeast + seaweed extract. The obtained results indicated that applying filter mud at all levels led to a significant increase in vegetative growth traits (stem length, stem diameter, leaves dry weight / seedling and stem dry weight / seedling) as well as , the elements of N, P and K %, except for the low level of filter mud (18 g/bag), in some cases. the highest values of these parameter were detected when adding filter mud at the high level (54 g /bag). Foliar spray with the examined stimulant Substances, in many cases, either separately or mixed resulted a significant augment in the studied traits. Clearly, bitter results were noticed by treating the seedlings with the triple combined treatment (amino acids + active yeast + seaweed extract) and the double combined treatment (amino acids + seaweed extract). the most of combined treatments significantly increased the growth traits and N, P and K %. Obviously, the most effective combined treatments of examined characters, in many cases were detected from supplying the seedlings with the high level of filter mud (54 g/bag) followed by filter mud at the moderate one (36 g/ bag) in combination with the triple combined treatment (amino acids + active yeast + seaweed extract) and the double combined treatment (amino acids + seaweed extract).