Organization of Extracellular Matrix Components During Differentiation of Adipocytes in Long-Term Culture

KUBO, YASUAKI; KAIDZU, SACHIKO; NAKAJIMA, IKUYO; Takenouchi, Kazuaki; Nakamura, Fumio

doi:10.1290/1071-2690(2000)036<0038:ooemcd>2.3.co;2

Cited by 41 publications

(45 citation statements)

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“…These newly accumulated extracellular matrix (ECM) macromolecules are comprised of various numbers of collagens, structural glycoproteins and proteoglycans. As previously reported in both mouse and bovine adipocytes (Kubo et al 2000, Nakajima et al 2002a, we also observed the same phenomenon with porcine adipocytes in which a prominent fibrillar network of collagens developed only in differentiated cells, thus forming fat cell clusters (unpublished data). In addition, impairing these ECM networks by treatment of ethyl-3,4-dihydroxybenzoate, a specific inhibitor of collagen synthesis, reduced the level of adipogenesis in mice and bovine (Ibrahimi et al 1992, Nakajima et al 2002b, as well as pig adipocytes (unpublished data).…”

Section: Extracellular Changes During Adipose Conversionsupporting

confidence: 89%

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Nakajima

2015

JARQ

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We have established a clonal porcine subcutaneous preadipocyte cell line for the first time. This cell line, named PSPA, shows a fibroblast-like phenotype and keeps on growing under growth medium even after reaching confluence. Adipose conversion occurs only when confluent cultures are stimulated with insulin, dexamethasone, biotin, pantothenate, and octanoate. Following exposure to this differentiation medium, growth was arrested immediately without mitotic expansion and PSPA cells exhibited a marked increase in intracellular lipid accumulation accompanied by changes in gene expressions directly related to fat metabolism and also in extracellular environments. Since PSPA cells were established, it has become possible to end the discussion over whether there is species specificity in the adipose conversion between pig and mouse by comparing PSPA cells and 3T3-L1 cells, the most widely studied preadipocyte cell line. Consequently, we demonstrated that responsiveness to inducers and fatty acid composition was unequal, despite being treated with the same media. Thus, species-specific differences indeed exist in adipogenesis between these two, meaning that PSPA cells would be an in vitro model specialized to improve understanding of pig adipocyte growth and differentiation in controlling the carcass fat content of pigs.

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Section: Extracellular Changes During Adipose Conversionsupporting

confidence: 89%

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Nakajima

2015

JARQ

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“…It should be noted that this signal was measured in the same cells in which the mTOR-Raptor signal was recorded. Single-cell data is of importance, because the adipose-derived stem cells changed, in addition to the lipid content, their morphology from fibroblast-like to spherical during the differentiation to mature adipocytes (33). The visible differences in cell morphology can be observed in Fig.…”

Section: Resultsmentioning

confidence: 99%

In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip

Schneider

Platen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Mammalian target of rapamycin (mTOR) is a central kinase integrating nutrient, energy, and metabolite signals. The kinase forms two distinct complexes: mTORC1 and mTORC2. mTORC1 plays an essential but undefined regulatory function for regeneration of adipose tissue. Analysis of mTOR in general is hampered by the complexity of regulatory mechanisms, including protein interactions and/or phosphorylation, in an ever-changing cellular microenvironment. Here, we developed a microfluidic large-scale integration chip platform for culturing and differentiating human adiposederived stem cells (hASCs) in 128 separated microchambers under standardized nutrient conditions over 3 wk. The progression of the stem cell differentiation was measured by determining the lipid accumulation rates in hASC cultures. For in situ protein analytics, we developed a multiplex in situ proximity ligation assay (mPLA) that can detect mTOR in its two complexes selectively in single cells and implemented it on the same chip. With this combined technology, it was possible to reveal that the mTORC1 is regulated in its abundance, phosphorylation state, and localization in coordination with lysosomes during adipogenesis. High-content image analysis and parameterization of the in situ PLA signals in over 1 million cells cultured on four individual chips showed that mTORC1 and lysosomes are temporally and spatially coordinated but not in its composition during adipogenesis.microfluidics | stem cell differentiation | adipogenesis | mTORC1 regulation | multiplexed PLA

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“…Fibronectin and collagens are the most abundant proteins of the ECM; however, the exact composition of the ECM in adipose tissue changes during development and is different between depots (Kubo et al, 2000;Mariman and Wang, 2010;Mori et al, 2014). Subcutaneous adipocytes of adult Wistar rats express high levels of type I, III and V collagen, whereas visceral adipocytes express high levels of collagen IIIIa1, laminin β1 (lamb1), laminin subunit γ1 (lamc1) and fibronectin 1 (FN1) (Mori et al, 2014) (Table 1).…”

Section: Remodeling Of the Ecm Is A Prerequisite For Adipose Tissue Ementioning

confidence: 99%

Heterogeneity of adipose tissue in development and metabolic function

Schoettl

Fischer

Ussar

2018

Journal of Experimental Biology

177

139

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Adipose tissue is a central metabolic organ. Unlike other organs, adipose tissue is compartmentalized into individual depots and distributed throughout the body. These different adipose depots show major functional differences and risk associations for developing metabolic syndrome. Recent advances in lineage tracing demonstrate that individual adipose depots are composed of adipocytes that are derived from distinct precursor populations, giving rise to different populations of energy-storing white adipocytes. Moreover, distinct lineages of energy-dissipating brown and beige adipocytes exist in discrete depots or within white adipose tissue depots. In this Review, we discuss developmental and functional heterogeneity, as well as sexual dimorphism, between and within individual adipose tissue depots. We highlight current data relating to the differences between subcutaneous and visceral white adipose tissue in the development of metabolic dysfunction, with special emphasis on adipose tissue expansion and remodeling of the extracellular matrix. Moreover, we provide a detailed overview of adipose tissue development as well as the consensus and controversies relating to adult adipocyte precursor populations.

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Organization of Extracellular Matrix Components During Differentiation of Adipocytes in Long-Term Culture

Cited by 41 publications

References 0 publications

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

Characterization of PSPA Cell Differentiation,an Established Pig Preadipocyte Cell Line as an In Vitro Model for Pig Fat Development

In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip

Heterogeneity of adipose tissue in development and metabolic function

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