Organization of the human FK506-binding immunophilin FKBP52 protein gene (FKBP4)

Scammell, Jonathan G.; Hubler, Tina R.; Denny, Wesley B.; Valentine, Donna L.

doi:10.1016/s0888-7543(03)00090-9

Cited by 21 publications

(24 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, FKBP52 interacts with HSP90, thereby facilitating the intracellular trafficking of steroid receptors. Moreover, this protein is involved in the regulation of interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes [56]. Here, we observed alterations in both domains, suggesting that this protein has a potential role in climate change adaptation in these species.…”

Section: Discussionmentioning

confidence: 56%

See 1 more Smart Citation

Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario

et al. 2017

View full text Add to dashboard Cite

Current knowledge on the biological responses of freshwater fish under projected scenarios of climate change remains limited. Here, we examine differences in the protein configuration of two endemic Iberian freshwater fish species, Squalius carolitertii and the critically endangered S. torgalensis that inhabit in the Atlantic-type northern and in the Mediterranean-type southwestern regions, respectively. We performed protein structure modeling of fourteen genes linked to protein folding, energy metabolism, circadian rhythms and immune responses. Structural differences in proteins between the two species were found for HSC70, FKBP52, HIF1α and GPB1. For S. torgalensis, besides structural differences, we found higher thermostability for two proteins (HSP90 and GBP1), which can be advantageous in a warmer environment. Additionally, we investigated how these species might respond to projected scenarios of 3° climate change warming, acidification (ΔpH = -0.4), and their combined effects. Significant changes in gene expression were observed in response to all treatments, particularly under the combined warming and acidification. While S. carolitertii presented changes in gene expression for multiple proteins related to folding (hsp90aa1, hsc70, fkbp4 and stip1), only one such gene was altered in S. torgalensis (stip1). However, S. torgalensis showed a greater capacity for energy production under both the acidification and combined scenarios by increasing cs gene expression and maintaining ldha gene expression in muscle. Overall, these findings suggest that S. torgalensis is better prepared to cope with projected climate change. Worryingly, under the simulated scenarios, disturbances to circadian rhythm and immune system genes (cry1aa, per1a and gbp1) raise concerns for the persistence of both species, highlighting the need to consider multi-stressor effects when evaluating climate change impacts upon fish. This work also highlights that assessments of the potential of endangered freshwater species to cope with environmental change are crucial to help decision-makers adopt future conservation strategies.

show abstract

Section: Discussionmentioning

confidence: 56%

“…torgalensis . In contrast, pH per se did not affect the genes involved in protein folding, except for fkbp4 , which possesses peptidylprolyl isomerase activity [56] and whose catalysis may depend on environmental pH [67]. Therefore, the lack of gene expression response in S .…”

Section: Discussionmentioning

confidence: 99%

Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The organization of the Fkbp51 and Fkbp52 genes has been described, showing that hormone regulatory elements lie within intronic sequences distal to the promoter. [62][63][64] Expression of FKBP51 is strongly enhanced by glucocorticoids, [65][66][67] progestins, 68, whether they are differentially expressed in pathophysiological conditions like metabolic syndrome or obesity. FKBP51 shuttles from mitochondria to the nucleus in a PKA-dependent manner at the onset of adipocyte differentiation FKBP51 is present in the cytoplasm and mitochondria, 74 and upon oxidative stress the mitochondrial fraction of this IMM rapidly translocates to the nucleus protecting cells from apoptosis.…”

Section: High Molecular Weight Immunophilins In Adipocyte Differentiamentioning

confidence: 99%

Adipogenesis is under surveillance of Hsp90 and the high molecular weight Immunophilin FKBP51

et al. 2015

View full text Add to dashboard Cite

Adipose tissue plays a central role in the control of energy balance as well as in the maintenance of metabolic homeostasis. It was not until recently that the first evidences of the role of heat shock protein (Hsp) 90 and high molecular weight immunophilin FKBP51 have been described in the process of adipocyte differentiation. Recent reports describe their role in the regulation of PPARγ, a key transcription factor in the control of adipogenesis and the maintenance of the adipocyte phenotype. In addition, novel roles have been uncovered for FKBP51 in the organization of the architecture of the nucleus through its participation in the reorganization of the nuclear lamina. Therefore, the aim of this review is to integrate and discuss the recent advances in the field, with special emphasis on the roles of Hsp90 and FKBP51 in the process of adipocyte differentiation.

show abstract

“…On the other hand, FKBP51 is increased by glucocorticoids (Baughman et al 1997;Reynolds et al 1998;Wan and Nordeen 2002;Vermeer et al 2003), progestins (Kester et al 1997;Richer et al 2002;Wan and Nordeen 2002;Hubler et al 2003), and androgens (Amler et al 2000;Mousses et al 2001;Zhu et al 2001;Jiang and Wang 2003). The genomic organization of the human FKBP51 and FKBP52 protein genes (FKBP5 and FKBP4, respectively) has been described (Scammell et al 2001;Scammell et al 2003), but otherwise little is known of how these genes are regulated at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Intronic hormone response elements mediate regulation of FKBP5 by progestins and glucocorticoids

Hubler¹,

Scammell²

2004

Cell Stress Chaper

Self Cite

173

132

View full text Add to dashboard Cite

Expression of FKBP51, a large molecular weight immunophilin, is strongly enhanced by glucocorticoids, progestins, and androgens. However, the activity of a 3.4-kb fragment of the FKBP51 gene (FKBP5) promoter was only weakly increased by progestin and we show here that it is unresponsive to glucocorticoids and androgens. The entire FKBP5 was scanned for consensus hormone response elements (HREs) using MatInspector. We found that 2 regions of intron E, which are conserved in rat and mouse FKBP5, contain HRE-like sequences with high match scores. Deoxyribonucleic acid fragments (approximately 1 kb in length) containing these regions were amplified and tested in reporter gene assays for steroid responsiveness. One region of intron E of FKBP5 (pIE2) conferred both glucocorticoid and progestin responsiveness to 2 heterologous reporter genes, whereas the other, less-conserved region of intron E (pIE1) was responsive only to progestins. The inclusion of pIE1 upstream of pIE2 (pIE1IE2) enhanced progestin but not glucocorticoid responsiveness. None of the constructs containing intronic sequences was responsive to androgens. Mutation of the putative HREs within pIE1 and pIE2 eliminated hormone responsiveness. Electrophoretic mobility shift assays demonstrated that progesterone receptors (PR) bound to the HRE in pIE1, whereas both PR and glucocorticoid receptors interacted with the HRE in pIE2. These data suggest that distal intronic elements significantly contribute to transcriptional regulation of FKBP5 by glucocorticoids and progestins.

show abstract

Organization of the human FK506-binding immunophilin FKBP52 protein gene (FKBP4)

Cited by 21 publications

References 29 publications

Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario

Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario

Adipogenesis is under surveillance of Hsp90 and the high molecular weight Immunophilin FKBP51

Intronic hormone response elements mediate regulation of FKBP5 by progestins and glucocorticoids

Contact Info

Product

Resources

About