Organogenesis and efficient in vitro plantlet regeneration from nodal segments of Allamanda cathartica L. using TDZ and ultrasound assisted extraction of quercetin

Khanam, Mehrun Nisha; Anis, Mohammad

doi:10.1007/s11240-018-1416-7

Cited by 24 publications

(9 citation statements)

References 33 publications

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“…The mean shoot number was approximately 37.5 ± 1.34 per explant with 100% induction; thus, we speculate that the presence of auxin facilitates the elongation of buds to balance the reduced dominance of the apical meristem under TDZ. Both the concentration and the exposure duration of TDZ for the explants in uenced the frequency of shoot proliferation and elongation (Khanam et al, 2018;Kumar et al 2018), and this result has been reported in numerous plants species, such as Rauwolfa tetraphylla (Faisal et al 2005) and Withania somnifera (Fatima and Anis 2011). When cultured on MS basal media with TDZ, the differentiated adventitious bud failed to elongate, even after 2 weeks of incubation (Fig.…”

Section: Discussionmentioning

confidence: 55%

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Background: As a highly valued medicinal plant, Salvia plebeia R. Brown belongs to the Lamiaceae family that has been subjected to over exploitation in its natural habitat for phytochemical and pharmacological studies. Alternative collection methods need to be developed for the large-scale propagation of Salvia plebeian.Results: Here, e cient and simple, direct organogenesis (from shoot tips and cotyledonary nodes explants) and Globular bodies (GBs) induction (from hypocotyl explants) systems were developed for the in vitro propagation of Salvia plebeia. The highest and number of regenerated shoots (7.0±0.82) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L -1 indole-3-acetic acid (IAA) and 1.0 mg L -1 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L -1 IAA and 1.0 mg L -1 thidiazuron (TDZ) yielded the maximum number of shoots (37.5±1.34) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GBs induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L -1 ) and IAA (0.1 mg L -1 ), and produced GBs that were morphologically similar to globular embryos and successfully germinated on hormone-free MS medium. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate.Conclusions: Taken together, this protocol can be e ciently used for mass propagation, germplasm preservation and likely also for gene transfer of Salvia plebeia.

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Section: Discussionmentioning

confidence: 55%

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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“…The elimination of browning associated with in vitro culture is vital for successfully establishing cultures [ 24 ]. The PGR with the highest efficiency is TDZ, a phenyl urea derivative considered as the current number-one-ranking synthetic cytokinin for regeneration and somatic embryogenesis [ 27 , 28 ]. Our observations are in line with high-cytokinin emerging species beneficial for the somatic embryogenesis induction [ 21 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Yang

et al. 2021

Plants

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This work was aimed at establishing an effective approach for in vitro propagation of Ajuga lupulina Maxim, a medicinal and ornamental plant mainly found in eastern Xizang, in the western Sichuan region of China. We report an optimum response in the proliferation of axillary shoots from nodal segment explants (10.2 shoots/explant) on MS medium containing 3.0 mg L−1 of 6-benzyladenine (BA). When BA and TDZ individually or in combination with NAA were employed for adventitious shoot regeneration, shoots and embryo-like structures (ELSs) were noted in the callus from leaf explants. The maximum response of 26.4 shoots /explant (81.6%) and 12.0 ELSs/explant were ascertained on MS medium with 4.0 mg L−1 TDZ and 0.1 mg L−1 NAA. The leaf despite browning still demonstrated a high regeneration capacity. TDZ (2.0 mg L−1) and BA (2.0 mg L−1) along with NAA (0.01 mg L−1) were found to perform well for shoot regeneration via callus from shoot tip explants. The best for rooting was MS medium (half-strength) containing indole-3-butyric acid (IBA: 1.5 mg L−1) and (NAA: 0.5 mg L−1) with the maximum number of roots (25.8 per shoot) and the highest rooting frequency (81.71%). The survival of the plantlets in the greenhouse was 78.2% indicative of successful acclimatization. This work is the first report of a consistent, definitive, and unique protocol for A. lupulina regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.

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“…TDZ is a phenyl urea-type cytokinin that mimics the activity of both auxin and cytokinin, which are the most preferably used cytokinins in some woody plant regeneration systems (Dewir et al 2018;Rukaya et al 2021). Previous studies described TDZ was used solely for indirect organogenesis and proved more effective for the shoot induction process in a range of plants in vitro (Khanam et al 2018;Wu et al 2020;Syeed et al 2021). However, in our study, TDZ in combination with KT was more conducive to the direct adventitious buds formation of Korean pine, which can be explained by the fact that TDZ is highly stable and regulates endogenous hormones, and shows the activity of both auxins as well as cytokinin, similarly mentioned in the literature (Dewir et al 2018;Dey et al 2012) also found that TDZ appears to stimulate cells in the apical meristem to divide, multiply, and develop so that bud differentiation occurred.…”

Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantsmentioning

confidence: 99%

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was successfully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg • L −1 kinetin (KT) in combination 0.5 mg • L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg • L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg • L −1 ), and activated charcoal (AC; 1 g • L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg•L −1 NAA and 0.5 or 1 mg • L −1 indole-3-butyric acid (IBA).Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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Organogenesis and efficient in vitro plantlet regeneration from nodal segments of Allamanda cathartica L. using TDZ and ultrasound assisted extraction of quercetin

Cited by 24 publications

References 33 publications

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

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