Organogénesis in-vitro using three tissues types of tree tomato [ Solanum betaceum (Cav.)]

Abstract

Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic trans-formation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots.

Tomato (Solanum lycopersicum L.) and Fusarium oxysporum f. sp. lycopersici interaction. A review

“…In this regard, some authors indicate that it may have originated in Andes mountains, specifically in Ecuador, where a large yet unexplored genetic diversity is found (Morales et al 2014). This vegetable has been a model of study in various areas such as physiology (Rodríguez-Ortega et al 2019), biochemistry (Trong et al 2019) and biotechnology (Ali et al 2014;Murillo-Gómez et al 2017); it has also been described as an agronomic model in genetic engineering and an important resource in tissue culture (Gerszberg et al 2015).…”

Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav.

“…In our study, BAP treatment (in the control condition) is effective to achieve shoot regeneration from tamarillo leaf explants. Furthermore, other studies have already demonstrated TDZ and BAP as good plant growth regulators to promote DNSO from leaf explants in tamarillo [31][32][33][34]. Recently, Shin et al [53] demonstrated that ethylene facilitates cell dedifferentiation and auxin-induced callus formation by regulating the abundance of transcripts for auxin receptor genes.…”

“…Solanum betaceum Cav., commonly known as tamarillo, is an Andean solanaceous tree, that has been used as a model system to study several micropropagation/regeneration processes, such as organogenesis [31][32][33][34] and somatic embryogenesis [35][36][37]. It has allowed a better understanding of these systems, and the possibility of further applications in other species to optimize protocols and regenerate adult selected trees [38].…”

“…In tamarillo, the effect of plant growth regulators, such as auxins and/or cytokinins on DNSO was already tested [31][32][33][34]. For instance, thidiazuron (TDZ) [31,32], benzylaminopurine (BAP) [31,33,34] or combinations of BAP and naphthaleneacetic acid [33,34] demonstrated to be the most suitable inducers of this process.…”

“…In tamarillo, the effect of plant growth regulators, such as auxins and/or cytokinins on DNSO was already tested [31][32][33][34]. For instance, thidiazuron (TDZ) [31,32], benzylaminopurine (BAP) [31,33,34] or combinations of BAP and naphthaleneacetic acid [33,34] demonstrated to be the most suitable inducers of this process. In terms of initial explant, leaves proved to be the most effective explant for the induction of shoot regeneration, when compared to petioles or root seedlings [31,33].…”