Organoid and microfluidics-based platforms for drug screening in COVID-19

Ramezankhani, Roya; Aminian, Mahdi; Chai, Yoke Chin; Vosough, Massoud; Verfaillie, Cathérine

doi:10.1016/j.drudis.2021.12.014

Cited by 30 publications

(23 citation statements)

References 105 publications

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“…Precise gene editing to simulate allelic variation in organoids through CRISPR can be used to screen for SARS-CoV-2 susceptibility genes. In addition, CRISPR can also identify the role of host proteins or signaling pathways in SARS-CoV-2-mediated pathophysiology through gene knockout studies or by utilizing CRISPRa/I, which allows gene activation or repression [ 91 ]. Using CRISPR, Dobrindt et al demonstrated that a single nucleotide polymorphism (SNP) in the furin gene is associated with SARS-CoV-2 infection in human iPSCs [ 92 ].…”

Section: Studies On Sars-cov-2 With Lung Organoidsmentioning

confidence: 99%

“…Computational platforms for drug screening can rapidly screen drug candidates for efficacy against COVID-19. At the same time, organoid models are also needed to study the efficacy and toxicity of these drugs [ 91 ]. Large-scale current good manufacturing practice (cGMP) grade production of organoids enables the future manufacture of “transplantable” organoids and tissues, opening up the potential of organoids as advanced therapy medicinal products (ATMPs) [ 99 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Lung Organoids as Model to Study SARS-CoV-2 Infection

et al. 2022

Cells

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Coronavirus disease-2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic and has severely affected socio-economic conditions and people’s life. The lung is the major target organ infected and (seriously) damaged by SARS-CoV-2, so a comprehensive understanding of the virus and the mechanism of infection are the first choices to overcome COVID-19. Recent studies have demonstrated the enormous value of human organoids as platforms for virological research, making them an ideal tool for researching host–pathogen interactions. In this study, the various existing lung organoids and their identification biomarkers and applications are summarized. At the same time, the seven coronaviruses currently capable of infecting humans are outlined. Finally, a detailed summary of existing studies on SARS-CoV-2 using lung organoids is provided and includes pathogenesis, drug development, and precision treatment. This review highlights the value of lung organoids in studying SARS-CoV-2 infection, bringing hope that research will alleviate COVID-19-associated lung infections.

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Section: Studies On Sars-cov-2 With Lung Organoidsmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Lung Organoids as Model to Study SARS-CoV-2 Infection

et al. 2022

Cells

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“…They can also properly and adequately recapitulate the physiological microenvironment of the tissue/organs of the body and, therefore, are able to successfully model SARS-CoV-2 virus infection and replication for the development of new drugs or vaccines to eventually manage COVID-19 ( Figure 7 a). The most frequently used 3D in vitro models include hydrogels, 71 organoids, [70] , [72] , [73] , [74] spheroids, 75 3D bioprinted constructs, [76] , [77] nanofibrous scaffolds, 78 microfluidics-on-chips, 72 among others. For instance, microfluidics-on-a-chip or organoid models loaded with multiple cell lineages together with nanosponges are potential platforms for the early diagnosis of symptoms related to multiorgan infection.…”

Section: Engineered In Vitro 3d-nanosponge Models ...mentioning

confidence: 99%

Nanosponges: An overlooked promising strategy to combat SARS-CoV-2

Mostafavi

Iravani

Varma

2022

Drug Discovery Today

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“…Ramachandran et al, [9] have studied the SARScoronavirus 2 (SARS-CoV-2) exposure using rapid detection of 35 minutes after receiving a test sample of raw nasopharyngeal swab using a microfluidic chip. Furthermore, Ramezankhani et al, [10] stated the potential of microfluidic devices for studying SARS CoV-2 and drug development to overcome this issue.…”

Section: Introductionmentioning

confidence: 99%

Numerical Investigation of Double Emulsion Droplets using Modified Flow Focusing Microfluidic Device for Drug Delivery

Raynaldo

Irwansyah²

2022

CFDL

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Double emulsion droplets generation is convenient for drug delivery applications since the core-shell template has the ability to increase the success of the target and release of the drug. In this study, the modified flow-focusing microfluidics device is proposed to generate double emulsion droplets with high monodispersity and high throughput, satisfying industrial needs. The W/O/W (water-in-oil-in-water) encapsulation template is a common combination in drug delivery. The research aims to analyze double emulsion droplets generation using the 2D Volume of Fluid (VoF) VoF approach, which is able to visualize flow regime, droplets average diameter, Coefficient of Variation (CoV), and droplets generation rate. Combination of water-in-olive oil-in-water was used as the working fluids. The diameter of the droplets, CoV, and generation rate was obtained using image processing. The simulation results showed that the injection model and the sudden expansion in the modified flow-focusing device successfully produced double emulsion droplets with two dripping instabilities. The narrowing jetting flow regime is obtained, including its droplets evolution. The average diameter for both outer and inner droplets were achieved with their CoV, including the generation rate. The outer and inner droplet's diameter generated can potentially be implemented for drug delivery, although the inner droplet's monodispersity must be further investigated. Nevertheless, the proposed device and the flow control were able to generate high-throughput double emulsion droplets.

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Organoid and microfluidics-based platforms for drug screening in COVID-19

Cited by 30 publications

References 105 publications

Lung Organoids as Model to Study SARS-CoV-2 Infection

Lung Organoids as Model to Study SARS-CoV-2 Infection

Nanosponges: An overlooked promising strategy to combat SARS-CoV-2

Numerical Investigation of Double Emulsion Droplets using Modified Flow Focusing Microfluidic Device for Drug Delivery

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