Original article Farming system affecting the in vitro activity of a Hippophaerhamnoides L. extract on phagocytosis in mastitic cows

Abstract

Innate cell-media ted defense mechanisms inclu de neu trophils as importa nt factors during phagocytosis and killing of the bacteria. Their presence is considered to be crucial in controlling the in situ infection, especially in high yielding cows. The research was carried out to evaluate the impact of clinical mastitis recorded under different farming technologies on the non-specific cell-mediated responses to Hippo pha e rha mno ide s L. a lcoholic extract and its potential therapeutic use.Randomly selected dairy cows showing clinical signs of mastitis were sampled for blood on two farms which were different in terms of raising technologies. Causative agents were identified from the milk by Sensititre Opti Read from Mueller Hinton agar plates. Carbon particle inclusion test was performed using an alcoholic Sea buckthorn extract to treat phagocytic cells. Phagocytic activity index was calculated as the difference between the natural logarithms of the optical densities of the phagocytosis divided by time (45 and 15 min, respectively). The statistical significance of the differences between the groups was interpreted by Student t-test.The results indicated that Streptococcus uberis represented the dominant bacterium on one farm while Staphylococcus aureus / E. coli were present in mastitic milk on the other. The spontaneous phagocytosis was increased in mastitic cows from the tie stall farm, while the response to the Hippophae extract significantly (p<0.0004) increased with 132.86% on the free range compared to the tie stall farm. The Sea buckthorn activity was stimulating in healthy cows on the free range farm, but negatively influenced by tie stall raising.The effects of the H. hamnoides alcoholic extract on the phagocytosis were conditioned not only by its composition but also by external factor complex, including the raising technology used on the farm.